Tenacibaculum ascidiaceicola sp. nov., isolated from the golden sea squirt Halocynthia aurantium

Abstract

A Gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated RSS1-6 T , was isolated from a golden sea squirt Halocynthia aurantium and its taxonomic position was investigated by using a polyphasic approach. Strain T grew optimally at 30-37 8C and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain RSS1-6 T fell within the clade comprising species of the genus Tenacibaculum, clustering with the type strains of Tenacibaculum discolor, Tenacibaculum litoreum and Tenacibaculum gallaicum with which it exhibited 16S rRNA gene sequence similarity values of 98.5-99.5 %. Strain RSS1-6 T contained MK-6 as the predominant menaquinone and iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) as the major fatty acids. The major polar lipids of strain RSS1-6 T were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G+C content was 32.5 mol% and the mean DNA-DNA relatedness values with the type strains of T. discolor, T. litoreum and T. gallaicum were 17.3-25.2 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RSS1-6 T is separated from other recognized species of the genus Tenacibaculum. On the basis of the data presented, strain RSS1-6

Tenacibaculum aquimarinum sp. nov., isolated from a marine alga and seawater

“…At the time of writing, the genus Tenacibaculum includes 31 validly and four invalidly published species (https://lpsn.dsmz.de/genus/tenacibaculum) that have been isolated from marine environments, particularly marine organisms [2–11]. Members of the genus Tenacibaculum generally form yellow-coloured colonies mainly by the production of zeaxanthin, not by flexirubin-type pigments, and their cells are Gram-stain-negative, aerobic and non-flagellated rods with gliding motility, containing a branched-chain unsaturated fatty acid, iso-C 15:0 and a hydroxylated fatty acid, iso-C 15:0 3-OH as the major cellular fatty acids and menaquinone (MK)-6 as the predominant isoprenoid quinone [2–11]. Most Tenacibaculum species are considered non-pathogenic, but some Tenacibaculum species have been reported to be associated with fish diseases [1–3].…”

“…The genus Tenacibaculum , belonging to the family Flavobacteriaceae in the phylum Bacteroidota , was first established through the reclassification of two bacterial species that were previously classified as species members of the genus Flexibacter [1]. At the time of writing, the genus Tenacibaculum includes 31 validly and four invalidly published species (https://lpsn.dsmz.de/genus/tenacibaculum) that have been isolated from marine environments, particularly marine organisms [2–11]. Members of the genus Tenacibaculum generally form yellow-coloured colonies mainly by the production of zeaxanthin, not by flexirubin-type pigments, and their cells are Gram-stain-negative, aerobic and non-flagellated rods with gliding motility, containing a branched-chain unsaturated fatty acid, iso-C 15:0 and a hydroxylated fatty acid, iso-C 15:0 3-OH as the major cellular fatty acids and menaquinone (MK)-6 as the predominant isoprenoid quinone [2–11].…”

Tenacibaculum sediminilitoris sp. nov., isolated from a tidal flat

“…Differential characteristics between strain YKTF-3 T and the type strains of six phylogenetically related Tenacibaculum species Growth at 10/40 C* +/- one unidentified lipid, one unidentified aminophospholipid and one unidentified glycolipid are major polar lipids, but distinguishable from those of the four reference strains by the presence of one unidentified aminolipid as a major component ( Fig. S4; Kim et al, 2016). The DNA G+C content of strain YKTF-3 T was 32.3 mol%, a value in the range reported for Tenacibaculum species (Table 1; Wang et al, 2008;Lee et al, 2009;.…”

“…At the time of writing, the genus Tenacibaculum comprises 22 species with validly published names (http://www.bacterio.net/tenacibaculum. html; Parte, 2014;Kim et al, 2016). Members of the genus Tenacibaculum have been isolated from a variety of marine environments and marine organisms (Suzuki et al, 2001;Frette et al, 2004;Yoon et al, 2005;Sheu et al, 2007;Heindl et al, 2008;Piñeiro-Vidal et al, 2008Wang et al, 2008;Lee et al, 2009;Oh et al, 2012;Li et al, 2013;Kim et al, 2013Kim et al, , 2016.…”

“…T and Tenacibaculum ascidiaceicola RSS1-6 T were used as reference strains for fatty acid analysis, and DNA of the six reference strains for DNA-DNA hybridization was obtained from previous studies (Yoon et al, 2005;Jung et al, 2006;Kim et al, 2016). Cell morphology, the Gram reaction, anaerobic growth and pH range for growth were determined as described by Park et al (2014).…”