Tensile properties of single stress fibers isolated from cultured vascular smooth muscle cells

Deguchi, Shinji; Ohashi, Toshiro; Sato, Masaaki

doi:10.1016/j.jbiomech.2005.08.026

Cited by 253 publications

(253 citation statements)

References 27 publications

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“…It is important to note that isolated/extracted SFs behave as a passive material [30], neglecting the critically important features of active myosin induced cross-bridge cycling and remodelling. The applicability of the active SF formulation used in the current study has been validated for a range of cell phenotypes and loading regimes [19,20].…”

Section: Discussionmentioning

confidence: 99%

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

et al. 2014

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Section: Discussionmentioning

confidence: 99%

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

et al. 2014

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“…To understand the physical pathway of intracellular force transmission, mechanical behaviors of individual cells [Dong et al 1991;Karcher et al 2003;Hashimoto et al 2004;Desprat et al 2005;Fernandez et al 2006;Kasza et al 2007] and subcellular structural components such as cytoskeletal filaments [Deguchi et al 2006] and the nucleus [Caille et al 1998;2002;Dahl et al 2004;2005;Tseng et al 2004;Deguchi et al 2005a;Rowat et al 2005;Vaziri et al 2006] have been investigated. The cytoskeletal filaments are thin protein fibers used for support of intracellular Keywords: cell biomechanics, microtensile test, mechanical properties, viscoelastic properties, nucleus, finite element method.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the mechanical properties of the nucleus inside a spherical endothelial cell based on microtensile testing

Deguchi

Yano

Hashimoto

et al. 2007

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The effect of extracellular forces on the nucleus deformation is an important research issue for better understanding of the intracellular force transmission mechanism. Approaches to this issue employing a microtensile test of single cells are helpful because the test enables one to give a well-controlled load onto the specimen with wide force and strain ranges. In the present study, tensile tests of single cells having a spherical shape are conducted by using a microtensile test system with a feedback control of displacement rate. Deformations of the nucleus inside the cell during the cell stretch and subsequent creep recovery after unloading are then quantified based on an image analysis. In order to characterize the creep recovery behaviors of the cell and its nucleus, one-dimensional analytical viscoelastic models and a power-law function are fitted to the creep recovery data. In addition, systematic finite element analyses are performed to estimate the intracellular stress distribution and elastic modulus of the cell and nucleus assumed to be continuum materials. These results indicate that the mechanical behaviors of the nucleus within a cell under stretching and unloading are similar to those under compression loadings previously reported.

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“…In cells, these numerous long F-ACTINs may represent bundles of actin filaments which gather together and form one or more stress fibres per focal adhesion. Long F-ACTINs were prestrained in the 3D model at 20 % consistently with the retraction of isolated stress fibre (Deguchi et al, 2006). Intermediate filaments are known to resist major deformations, whereas they are less involved in small cell deformations (Wang and Stamenović, 2000;Fudge et al, 2003).…”

Section: Description Of Csk Components By the Cdm Modelmentioning

confidence: 99%

“…One stress fibre was thus represented by a bundle of several long F-ACTIN interactions. For instance, long F-ACTIN was defined by a rigidity K of 1.10 -2 nN/ strain and a pre-strain τ fixed at 0.2 following the level of retraction observed in isolated stress fibres by Deguchi et al (Deguchi et al, 2006). Intermediate filaments form a dense and unstretched network around the nucleus and connect it to the actin CSK (Green et al, 1986).…”

Section: Constitutive Equationsmentioning

confidence: 99%

“…Understanding the cell differentiation that follows cell adhesion requires identifying the mechanotransduction pathways via the CSK and nucleoskeleton (NSK) in taking into account the role of intracellular tonus that appears as a signal activating mechanosensitive complexes. Several authors have studied experimentally the mechanics of cell adhesion by determining the magnitude of force at FAPs and the distribution of stress fibres (Green et al, 1986;Jean, 1999;Deguchi et al, 2006;Milan et al, 2007). Mechanical models based on tensegrity have also been proposed to relate the internal tension to the adhesion conditions (Kurachi et al, 1995;Balaban et al, 2001;Brangwynne et al, 2006;Dubois and Jean, 2006;McNamara et al, 2010).…”

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confidence: 99%

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Computational model combined with in vitro experiments to analyse mechanotransduction during mesenchymal stem cell adhesion

Milan¹,

Lavenus²,

Pilet³

et al. 2013

eCM

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The shape that stem cells reach at the end of adhesion process influences their differentiation. Rearrangement of cytoskeleton and modification of intracellular tension may activate mechanotransduction pathways controlling cell commitment. In the present study, the mechanical signals involved in cell adhesion were computed in in vitro stem cells of different shapes using a single cell model, the so-called Cytoskeleton Divided Medium (CDM) model. In the CDM model, the filamentous cytoskeleton and nucleoskeleton networks were represented as a mechanical system of multiple tensile and compressive interactions between the nodes of a divided medium. The results showed that intracellular tonus, focal adhesion forces as well as nuclear deformation increased with cell spreading. The cell model was also implemented to simulate the adhesion process of a cell that spreads on protein-coated substrate by emitting filopodia and creating new distant focal adhesion points. As a result, the cell model predicted cytoskeleton reorganisation and reinforcement during cell spreading. The present model quantitatively computed the evolution of certain elements of mechanotransduction and may be a powerful tool for understanding cell mechanobiology and designing biomaterials with specific surface properties to control cell adhesion and differentiation. Keywords:Mesenchymal stem cells; cell adhesion; cell morphology; computational cell adhesion model; cytoskeleton; mechanical forces; mechanotransduction. IntroductionCell adhesion is fundamental in the field of biomaterials. Especially in orthopaedics, it is crucial for prosthesis osseointegration (Lavenus et al., 2010). Osteoblasts need to adhere on the implant to synthesise the bone tissue that is directly connected to it without the intervening soft tissue. Moreover, stem cells, which are recruited to heal the implant interface, adhere on the surface and differentiate depending, among other factors, on their adherent morphology. For instance, stem cells which reach at the end of adhesion round, elongated or branched shapes depending on substrate topography or stiffness, differentiate preferentially into adipocytes, fibroblasts or osteoblasts, respectively (McBeath et al., 2004;Engler et al., 2006). Therefore, biomaterial surface must promote adhesion of stem cells and their differentiation into the desired phenotype so as to synthesise a periprosthetic tissue, ensuring efficient and viable biomaterial integration. Biomaterials that mimic the natural composition of the extracellular matrix have been developed. They are composed of a biological phase or are coated with RGD complex and showed positive effects on cell adhesion (Von der Mark et al., 2010). Topography and surface chemistry, which play a key role in cell adhesion, may be controlled using nanotechnology. This announces the development of new implant surfaces with predictable tissue-integrative properties (Le Guéhennec et al., 2007;Lavenus et al., 2010).Cell adhesion results from the creation of several discrete focal a...

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Tensile properties of single stress fibers isolated from cultured vascular smooth muscle cells

Cited by 253 publications

References 27 publications

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

On the role of the actin cytoskeleton and nucleus in the biomechanical response of spread cells

Assessment of the mechanical properties of the nucleus inside a spherical endothelial cell based on microtensile testing

Computational model combined with in vitro experiments to analyse mechanotransduction during mesenchymal stem cell adhesion

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