Test for Reovirus-Like Agent

Spence, L.; Fauvel, M.; Bouchard, S. Maldonado; Babiuk, Lorne A.; Saunders, J. R.

doi:10.1016/s0140-6736(75)92752-x

Cited by 42 publications

(22 citation statements)

References 2 publications

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“…The presence of rotavirus antigen was also investigated by a reverse complement fixation test (RCF) (Spence et al 1975). A preparation of the patients' faeces was made by differential ultracentrifugation of an aqueous suspension of 1-5 g of stool, and this was then titrated against a fixed dilution of rabbit antiserum to the simian rotavirus SAI1.…”

Section: Virological Investigationsmentioning

confidence: 99%

A microbiological investigation of acute summer gastroenteritis in Black South African infants

Schoub

Greeff

Lecatsas

et al. 1977

J. Hyg.

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SUMMARYA microbiological investigation of Black infants suffering from severe acute summer gastroenteritis revealed enteropathogenic agents in 30 out of 37 patients (81 %). Enterotoxigenic bacteria were isolated from 15 patients (41 %). A total of 16 enterotoxigenic strains were isolated, comprising 9 enterotoxigenic Escherichia coli strains secreting labile and stable toxin on their own and in combination, and labile-toxin secreting strains of Klebsiella pneumoniae (4), Enterobacter cloacae (2) and Proteus vulgaris (1). In the case of the latter three species, however, 6 out of the 7 strains were isolated from patients who were excreting other enteric pathogens, whereas only 2 out of 9 enterotoxigenic E. coli patients had concomitant infections with other pathogens. No invasive bacteria were isolated except for 2 sbigella strains. Salmonella and shigella strains were found in four patients. No correlation was found between the enteropathogenicity of E. coli and its serotype. Rotavirus was observed by negative staining electron microscopy in only two patients (6 %) but using a reverse complement fixation test rotavirus antigen was detected in the stool of 17 out of 35 patients (49 %). The low EM detection rate may well be due to the patients being admitted for treatment late in the course oftheir illness when the degree of viral shedding has decreased below EM detectability. No significant difference in clinical presentation was noted between the various aetiological agents. Only one patient was being solely breast-fed compared to 16 % of control non-diarrhoeic infants. Evidence of malnutrition was noted in over half of our patients.

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Section: Virological Investigationsmentioning

confidence: 99%

A microbiological investigation of acute summer gastroenteritis in Black South African infants

Schoub

Greeff

Lecatsas

et al. 1977

J. Hyg.

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“…This is a simple technique provided an electron microscope is available. Counter-immunoelectrophoresis is a technique to detect viral antigen in the stool (Spence et al, 1975;Middleton et al, 1976). It seems to be about as sensitive as the electron microscope.…”

Section: Diagnostic Techniquesmentioning

confidence: 99%

Rotavirus gastroenteritis.

Walker-Smith¹

1978

Archives of Disease in Childhood

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“…Although in-vitro replication has been achieved in human foetal intestinal organ culture and in cell culture (Banatvala et al, 1975;Purdham et al, 1975;Wyatt et al, 1976), the yield of virus has been low. The calf rotavirus, which has been adapted to grow in cell culture, cross-reacts antigenically with the human agent by complement fixation, immunofluorescence, immunoelectronmicroscopy and counterimmunoelectrophoresis, and has thus been widely used for serodiagnosis of human rotavirus infection (Flewett et al, 1974;Kapikian et al, 1974Kapikian et al, , 1975Echeverria, Blacklow and Smith, 1975;Spence et al, 1975). Its possible use for immunoprophylaxis has also been suggested (Kapikian et al, 1975).…”

Section: Plate Imentioning

confidence: 99%