Test of Antioxidant Activity Leaves of Scaevola Taccada (Gaertn.) Roxb. Using DPPH (1, 1-Diphenyl-2-Picrylhydrazyl)

Rahmawati,; Rahman, Safriani; Wati, Aulia; Herman, Hendra; Arsyad, Fauzan

doi:10.7897/2230-8407.050333

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…were grinded and filtered with the 40 mesh size to gain refined powders, and then they were repeatedly macerated using 70% ethanol for 3 days. Afterward, the macerated leaves evaporated using a rotary Rotavapor until formed congealed extracts, and then they were weighted with 400 mg/gr body weight [2], [6].…”

Section: Extraction Processmentioning

confidence: 99%

“…In addition, it has significant functions as antitumor, anti-inflammatory, antibacterial, antifungal, antioxidant, and antiviral effects. Local people generally process this plant as the traditional medicine by boiling method, and directly put the concoction of this plant on injured parts of the body in the form of squeezed juices [4], [5], [6], [7]. IL-1β belongs to a member of the IL-1 family that plays significant functions as a pro-inflammatory cytokine, and it has agonist activity.…”

Section: Introductionmentioning

confidence: 99%

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Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

Prihantono¹,

Syamsu²,

Smaradhania³

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Mastitis is an inflammation of the breast tissue, usually caused by bacteria. Mastitis stimulates pro-inflammatory cytokines. The cytokine interleukin-1β (IL-1β) is a crucial mediator of the inflammatory response. This cytokine has adverse effects of hosting immunity that mediates resistance to pathogens and also exacerbates damage during chronic disease and acute tissue injury. Scaevola taccada (Gaertn.) Roxb. has been used as an ethnomedicine for healing sores in several provinces in Indonesia. AIM: This study aimed to assess the profile of pro-inflammatory cytokine IL-1β through the treatment effect of leaf extracts of S. taccada (Gaertn.) Roxb. as adjuvant for healing mastitis. METHODS: This study was a true control group experiment using the pre-test-post-test control design aimed to measure the effect of hydroalcoholic compounds in leaf extracts of S. taccada on the systemic pro-inflammatory activity of interleukin-1β (IL-1β). The treated animals were 18 mice of Sprague Dawley strain induced by Staphylococcus aureus. These treated mice were divided into three groups in which each group consisted of six mice. The mice in the Group I (negative control) were given 1 ml aquabides/250 g body weight, those in the Group II (positive control) were delivered with 9.6 mg/ml amoxicillin/250 g body weight, and those in the Group III (experimental) were given 9.6 mg amoxicillin/250 g body weight + 400 mg/ml leaf extracts of S. taccada/g body weight for 5 days, respectively. Pathological examinations were carried out from the inflamed tissues to prove the healing process of the treated mice. IL-1Β levels were determined using the enzyme-linked immunosorbent assay method. Data were analyzed using ANOVA and post hoc tests. RESULTS: There were statistically significant differences of IL-1β levels after the administration of leaf extracts of S. taccada among all the treated mice groups at p < 0.05. The Group III had the lowest IL-1β level with the mean value ± 1.45 pg/ml compared to the IL-1β level in the Group II (positive control) with the mean value ± 3.82 pg/ml and the IL-1β level in the Group I (negative control) with the with mean value ± 5.22 pg/ml. The pathological analysis of breast tissues of the treated mice proved that leaf extracts of S. taccada (Gaertn Roxb.) could reduce damaged tissues, cellular infiltration, and subcutaneous edema induced by this pathogenic microorganism. CONCLUSION: Leaf extracts of S. taccada had a significant function as adjuvant for healing mastitis by reducing pro-inflammatory cytokine IL-1β.

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Section: Extraction Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

Prihantono¹,

Syamsu²,

Smaradhania³

et al. 2020

Open Access Maced J Med Sci

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“…The diluents were prepared as a series of 100, 150, 200, and 250 µL and dropped into the Oxicount-Antioxidant kit test plate, followed by adding methanol up to the total volume of 300 µL and measured the wavelength at 517 nm by Microplate reader UV-visible Spectrophotometer. The diluents 100, 150, 200, and 250 µL in the total volume 300 µL were calculated to the initial weight of rubber concentration; 25, 37.5, 50, 63.5 µg/µL and plotted between the concentration (as the x axis) and % DPPH scavenging activity (as the y axis), EC50 was calculated by using a linear regression equation y = ax+b as shown in the Eq.3 [11].…”

Section: Determination Of the Term Half Maximal Effective Concentrati...mentioning

confidence: 99%

Effect of Various Extracted Solvent on DPPH Radical Scavenging Activity of Natural Rubber

Siriwong

Rungvichaniwat

Klinpituka

2015

KEM

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Four types of extracted solvents i.e. mixtures of chloroform:acetone, chloroform:methanol, cyclohexane:acetone and cyclohexane:methanol, in the volume ratio of 4:1 were used to dissolve the natural rubber. Two grades of natural rubber i.e. air dry sheet (ADS) and ribbed smoked sheet No.3 (RSS3) were investigated. The rubber solution was purified by separated the rubber out with methanol. The non-rubber solution was then quantitative analyzed to verify 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity using Oxicount-Antioxidant kit test and also calculated the term of half maximal effective concentration (EC50). The plasticity retention index (PRI) was also investigated in order to compare the ageing properties of the rubber and their antioxidant activity. Furthermore, various rubbers i.e. ADS, RSS3 and Standard Thai Rubber 20 (STR20) were collected during early period of yearly tapping seasons to analyze the DPPH radical scavenging activity and EC50. It was found that the mixture of cyclohexane and methanol showed the highest DPPH radical scavenging activity and lowest EC50. RSS3 showed higher DPPH radical scavenging activity than that of ADS which the higher antioxidant activity also displayed the higher PRI values. It also found that during the early period of yearly tapping seasons, DPPH radical scavenging activity of RSS3 was found higher than that of ADS and STR20, respectively when extracted by mixture of cyclohexane and methanol.

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Quantitative Analysis of Total Phenolic Contents and Antioxidant Activity of Fresh Field Natural Rubber Latex

Siriwong

Rungvichaniwat

Klinpituksa

et al. 2015

Macromolecular Symposia

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Summary: Dried films of fresh field natural rubber latex from Pattani local rubber plantation, Thailand were prepared. The film (1g) was dissolved by various solvent (100ml) i.e. hexane, chloroform and mixtures of chloroform: methanol in the ratio of 1:1, 2:1 and 4:1. The solution was purified by separated the rubber out with methanol. The non-rubber solution was then quantitative analyzed to verify total phenolic content (TPC), ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity using Oxicount Antioxidant kit test. It was found that only hexane, chloroform and mixture of chloroform: methanol of ratio 4:1 can dissolve rubber film completely. The non-rubber content yields after evaporated solvent were 8. .42 AE 0.05%, respectively. This study found that TPC and antioxidant activity of rubber film clearly depend on the type of dissolved solvent. The highest antioxidant activity from this study was found by using the mixture of chloroform: methanol in the ratio of 4:1. Furthermore, the phenolic acid content of natural rubber were reported using HPLC, based on which more phenolic acids were presented in the sample extracted by chloroform:methanol mixture.

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Test of Antioxidant Activity Leaves of Scaevola Taccada (Gaertn.) Roxb. Using DPPH (1, 1-Diphenyl-2-Picrylhydrazyl)

Cited by 4 publications

References 17 publications

Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

Effect of Various Extracted Solvent on DPPH Radical Scavenging Activity of Natural Rubber

Quantitative Analysis of Total Phenolic Contents and Antioxidant Activity of Fresh Field Natural Rubber Latex

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