Testicular spermatozoa have statistically significantly lower DNA damage compared with ejaculated spermatozoa in patients with unsuccessful oral antioxidant treatment

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

“…In the study presented above, testicular spermatozoa had a lower degree of DNA damage than ejaculated spermatozoa, which supports the previous reports [Greco et al 2005b;Moskovtsev et al 2010]. In the study by Greco and colleagues, testicular spermatozoa had DNA damage of 4.8% compared to 23.6% of ejaculated spermatozoa.…”

“…Patients with persistently high sperm DNA damage after an ineffective course of oral antioxidant treatment were referred to ICSI with testicular spermatozoa. The three-month course of antioxidant treatment of daily vitamins (C 500 mg, E 100 IUS, folic acid 1 mg) supplemented with 20 mg of zinc and 200 µg selenium was administered as previously reported [Moskovtsev et al 2010]. Ejaculated and testicular spermatozoa were obtained immediately prior to ICSI treatment, however the number of subjects was limited to eight patients who met the inclusion criteria for the study and had excess testicular samples which could be utilized for sperm DNA fragmentation and aneuploidy assessments.…”

“…The nature of DNA damage, failure of the antioxidant system, or enzyme production in some patients may be responsible for unsuccessful antioxidant treatment in some men, including patients in this group. It has been shown that testicular samples exhibit significantly lower DNA damage compared to ejaculate spermatozoa [Greco et al 2005b;Moskovtsev et al 2010].…”

Cryopreservation of Human Spermatozoa by Vitrification vs. Slow Freezing: Canadian Experience

“…Significant increase in percentage of DNA fragmentation was associated with an increase in oxidative stress during cryopreservation www.intechopen.com (Thomson et al, 2009). A slide-based technique for the assessment of sperm DNA was performed as previously described (TUNEL: TdT-mediated dUTP nick end labelling) (Moskovtsev et al, 2010) We found statistically significant increase in sperm DNA damage after both methods of sperm freezing (P < 0.05). However, the increase in DNA damage was minimal and to a degree probably irrelevant to clinical concerns.…”

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

“…По этиологии ФДС обсуждается 3 основные гипо-тезы: возникновение разрывов ДНК в результате на-рушения ремоделирования хроматина в спермиогене-зе [107,112]; ФДС как результат апоптоза, который включается в тестикулярной ткани (абортивный апоп-тоз) [113] или начинается после спермиации [114]; действие активных форм кислорода, которые могут влиять на сперматозоиды как в семенных канальцах, так и посттестикулярно [115,116].…”