Testicular torsion and reperfusion: Germ cell DNA damage and development

Talebi, Hatef; Farahpour, Mohammad Reza

doi:10.1111/and.13243

Cited by 13 publications

(24 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that free oxygen radicals cause I/R damage 15,16 . Also, due to tissue hypoxia, TT may cause germinal cell necrosis, decreased fertility, and even infertility 5,17 . Functional capacity of the testicle can be understood by hormonal status and spermatogenesis evaluation.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 Also, due to tissue hypoxia, TT may cause germinal cell necrosis, decreased fertility, and even infertility. 5,17 Functional capacity of the testicle can be understood by hormonal status and spermatogenesis evaluation. In an experimental study in rats by Turner et al, 18 testicles were torsioned 720 degrees for one hour, and the testicular vein testosterone levels of the non-torsioned contralateral testicles and torsioned testicles were compared on Days 3 and 30.…”

Section: Discussionmentioning

confidence: 99%

“…In TT, detorsion is of utmost importance to provide normal blood flow to the testicles. However, despite normal blood flow after detorsion, reperfusion into ischemic tissues may cause damage to the affected testicle and subsequent testicular dysfunction 5 . Moreover, ischemia/reperfusion (I/R) damage may also exert harmful effects on the contralateral testicle, depending on the duration of ischemia 6 .…”

Section: Introductionmentioning

confidence: 99%

“…However, despite normal blood flow after detorsion, reperfusion into ischemic tissues may cause damage to the affected testicle and subsequent testicular dysfunction. 5 Moreover, ischemia/reperfusion (I/R) damage may also exert harmful effects on the contralateral testicle, depending on the duration of ischemia. 6 Therefore, many agents such as N-acetyl cysteine and metformin have been administered to reduce I/R damage in TT.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of platelet‐rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model

et al. 2020

View full text Add to dashboard Cite

Background Platelet‐rich plasma is a biological instrument rich in growth factors and cytokines. Objectives The aim of this study was to investigate the effect of platelet‐rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model. Materials and methods The rats were randomly divided into three groups, including six rats in each group as follows: the first group as the sham group; the second group as the ischemia/reperfusion + Saline group and the third group as the ischemia/reperfusion + platelet‐rich plasma group. The left testicles of the ischemia/reperfusion + Saline and ischemia/reperfusion + platelet‐rich plasma group were kept in four‐hour torsion. Then, the left testicles of ischemia/reperfusion + Saline and ischemia/reperfusion + platelet‐rich plasma groups were detorsioned, and intra‐testicular 1 cc saline (ischemia/reperfusion + Saline) and 1 cc platelet‐rich plasma (ischemia/reperfusion + platelet‐rich plasma) were injected. At one month, blood samples were taken from all groups for hormonal evaluation and left orchiectomy was performed. Results The mean follicle‐stimulating hormone level of ischemia/reperfusion + Saline group was significantly higher than ischemia/reperfusion + platelet‐rich plasma group (7.78 ± 0.23 vs 6.18 ± 0.28 nmol/l, respectively, P = .004). The mean LH level of ischemia/reperfusion + platelet‐rich plasma group was significantly lower than ischemia/reperfusion + Saline group (3.63 ± 0.28 vs 5.68 ± 0.21 nmol/l, respectively, P = .004). The mean total testosterone level of ischemia/reperfusion + platelet‐rich plasma group was significantly higher than ischemia/reperfusion + Saline group (8.05 ± 0.24 vs 5.78 ± 0.23 nmol/l, respectively, P = .004). The mean Johnsen scores of ischemia/reperfusion + platelet‐rich plasma group were significantly higher than ischemia/reperfusion + Saline group (5.85 ± 0.58 vs 3.93 ± 0.65, respectively, P = .004). The mean Johnsen score of the sham group was significantly higher than ischemia/reperfusion + platelet‐rich plasma and ischemia/reperfusion + Saline groups (P = .003 and P = .003, respectively). Discussion and conclusion The platelet‐rich plasma has beneficial effects on spermatogenesis and reproductive hormone production in testicular torsion. It is easily accessible and applicable. In the future, intra‐testicular platelet‐rich plasma injection may be used in testicular torsion after detorsion. However, further experimental and large‐scale prospective clinical studies are needed to establish a definitive conclusion on this topic.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of platelet‐rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Studies have shown that free oxygen radicals are responsible for tissue ischaemia-reperfusion injury (Karaguzel, Kadihasanoglu, & Kutlu, 2014;Karaguzel, Sivrikaya, et al, 2014). Testicular torsion leads to tissue hypoxia, germinal cell necrosis and reduced fertility or infertility (Talebi & Farahpour, 2019;Tamamura et al, 2010). Experimental studies have shown that testicular necrosis develops within 2 hr in arterial occlusion and 6 hr in venous occlusion (Dubois & Dodat, 1998).…”

Section: Discussionmentioning

confidence: 99%

Predictive value of ischaemia‐modified albumin in spermatogenesis in an experimental testicular torsion model

et al. 2019

View full text Add to dashboard Cite

Our aim was to measure the ability of ischaemia-modified albumin (IMA) to predict testicular histopathological damage in the testes of rats with short-and long-term ischaemia using experimental testicular torsion and subsequent reperfusion via detorsion.21 Wistar Albino rats were randomized into three groups. The sham group was subjected to a mid-scrotal incision only. The 4-and 8-hr T/D (Torsion/Detorsion) groups were subjected to left testicular torsion by twisting the testes by 720 degrees counterclockwise. 2 cc venous blood samples were taken from the sham group after the mid-scrotal incision, and from the 4-and 8-hr T/D groups after 4 and 8 hr respectively. After that, the 4-and 8-hr T/D groups were subjected to detorsion.Two days later, orchiectomy was performed. Ischaemia-modified albumin levels were significantly different among the groups at 48 hr prior to orchiectomy (reperfusion; p = .003). Based on the results of the paired comparisons, it was found that IMA levels of the sham group were significantly higher than those of the 4-and 8-hr T/D groups (p = .002 and .009 respectively). Our study has showed that IMA may be used to predict ischaemia/reperfusion injury, which is another complication that may occur following detorsion in testicular torsion. K E Y W O R D Sexperimental testicular torsion, ischaemia, ischaemia-modified albumin, rats, reperfusion, spermatogenesis

show abstract

Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion

et al. 2021

View full text Add to dashboard Cite

Testicular torsion and reperfusion: Germ cell DNA damage and development

Cited by 13 publications

References 35 publications

Effects of platelet‐rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model

Effects of platelet‐rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model

Predictive value of ischaemia‐modified albumin in spermatogenesis in an experimental testicular torsion model

Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion

Contact Info

Product

Resources

About