Testis evaluation of adult Wistar rats after neonatal treatment with fluoxetine

Abstract

In current assay the serotoninergic system in newly-born Wistar rats underwent pharmacological modification by fluoxetine, a selective serotonin reuptake inhibitor (SSRI), to investigate its repercussion on testicular parameters in adult animals. Thirty animals were distributed according to treatment: control animals (n = 6), animals treated with 1 mg kg -1 (n = 6), 5 mg kg -1 (n = 6), 10 mg kg -1 (n = 6) and 20 mg kg -1 (n = 6) of fluoxetine (IP). When 150 days old, the animals were anesthetized and perfused intra-cardiacally with fixative solution. Testes were routinely processed for inclusion in plastic resin (methacrylate glycol). Further, 4 μm-thick histological sections were stained with toluidine blue/sodium borate 1% and analyzed histometrically. Pharmacological intervention on the serotoninergic system during the postnatal period of the testes development in Wistar rats with fluoxetine chlorohydrate reduced parameters, such as testicular weight, testis liquid weight and seminiferous tubules diameter. However, testicular parameters, such as daily sperm production (DSP), spermatogenesis efficiency (DSP/g/testis) and cell population in stage VII of adult animals, were not influenced by fluoxetine chlorohydrate usage during neonatal period. Results show that administration of fluoxetine during 21 days after birth may induce adverse changes in the spermatogenesis of adult rats.

White tea consumption restores sperm quality in prediabetic rats preventing testicular oxidative damage

“…In this study, STZ-treated rats presented mild hyperglycaemia, followed by glucose intolerance and insulin resistance, illustrating that these animals developed the main characteristics of prediabetes (Tabak et al, 2012). In this study, no significant differences were observed regarding gonad weight and gonadosomatic index, allowing us to infer that prediabetes per se did not cause major macroscopic dysfunction on rat testicular development and sexual maturation (Barber and Blake, 2006;da Silva Junior et al, 2012). In fact, some authors reported no alterations in testicular morphology or histoarchitecture of prediabetic rats, as well as in testicular weight (Kumara et al, 2011), as observed in this study.…”

Fluoxetine induces changes in the testicle and testosterone in adult male rats exposed via placenta and lactation

“…This may be due to the long time that the animals were not exposed to fluoxetine between puberty and adulthood. In addition, other studies also showed changes in spermatogenesis of rats even after a long period of time at doses of 5, 7.5, and 10 mg/kg of fluoxetine [Silva Junior et al 2013;Vieira et al 2013]. Therefore, doses above 5-10 mg/kg of fluoxetine should be considered carefully as they may represent a risk to the fertility of male rats.…”

“…Also, SSRI exposure via placenta and lactation may inhibit the testicular development in prepubertal rats and decrease the number of spermatozoa in adult rats [Oliveira et al 2013;Vieira et al 2012]. Adult male rats treated with SSRI also showed changes in spermatogenesis and Sertoli cell population [Aggarwal et al 2012;Silva Junior et al 2008;Silva Junior et al 2013]. Furthermore, the blockage of the serotoninergic system in prepubertal rats inhibits spermatogenesis development [Aragón et al 2005].…”

Evaluación de la maduración y calidad espermática epididimaria en ratas macho adultas expuestas a fluoxetina durante la gestación y lactancia

“…La FLX no solo altera la conducta sexual, sino también la cito arquitectura del testículo (Ramos et al, 2016;Silva Junior et al, 2013). Al respecto, se ha observado que ratas machos expuestas en el periodo gestacional y de lactancia con una dosis de 7.5 mg/kg de FLX administrada por vía intragástrica disminuye el número de células de Sertoli a los 21 y 50 días de edad (Ramos et al, 2016).…”

“…Las hembras se dividieron en dos grupos, a cada grupo se le administró vía intragástrica los siguientes tratamientos una vez al día; grupo control (0.5 ml de solución salina); y el grupo experimental al cual se le administró una dosis de 20 mg/kg de FLX, se utilizó esta dosis debido a que se ha reportado el mayor parte de los efecto a nivel testicular (Monteiro Filho et al, 2014;Ramos et al, 2016;Silva Junior et al, 2013), por otra parte, la mayoría de las presentaciones comerciales del fármaco manejan comprimidos con dicha concentración. Las crías machos fueron separadas de sus madres 25 días después del nacimiento, y a los 4 meses de edad fueron sometidos a los análisis calidad espermática y maduración epididimaria.…”