Tetrahymena pyriformis Phagocytic Activity Test for Rapid Toxicity Assessment of Aquatic Micropollutants

Fekete-Kertész, Ildikó; Ullmann, Orsolya; Csizmár, Panni; Molnár, Mónika

doi:10.3311/ppch.10667

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…In the case of bacteria, the most common species used in ecotoxicological studies is Allivibrio fischeri [60,87,93,94,98]. In the case of protozoa, Tetrahymena pyriformis [99], Stentor coeruleus, Stylonychia lemnae [100], or Paramecium caudatum [101], among others, are used. These tests are quite popular among the scientific community because these biomodels are more sensitive than vertebrate biomodels.…”

Section: Ecotoxicological Informationmentioning

confidence: 99%

Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

et al. 2022

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In the last half century, the improvements in quality of life owing to the development of the chemical industry are indisputable. However, despite global improvements, there has also been a large increase in pollution at the environmental level and this has caused relevant harmful risks not only to wildlife and the environment but also to human health. In response, governments have begun to regulate and control chemicals to prevent environmental pollution. At the European level, REACH (Registration, Evaluation, Authorization, and Restriction of Chemicals) was created with the aim to protect human/animal health and the environment from chemicals. Additionally, this regulation shows the main experimental tests that are needed to classify a chemical from a physicochemical and toxicological point of view. The main objective of this study is to compare the tests or experiments stipulated by the European REACH regulation with the studies carried out by the scientific community. To obtain this comparison, an exhaustive bibliographic review was carried out, analyzing the physicochemical properties and the (eco)toxicological information established by the European REACH regulation and scientific articles published in the Web of Science (WOS) database. The results obtained indicate that, although there are many authors who conducted tests indicated by the regulation, there are others whose essays or studies are not in line with the regulation; this may be because, on many occasions, the purpose of the information to be obtained is quite different.

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Section: Ecotoxicological Informationmentioning

confidence: 99%

Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

et al. 2022

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“…Comparative to survival bioassays, behavioral effects are considered to be the most sensitive indicators of environmental changes because they occur quickly and can be detected at low doses and in non-invasive ways on living organisms [ 27 ]. In Tetrahymena , behavior studies mainly focus on phagocytosis activity [ 28 ], chemotaxis response [ 29 ], and cell motility [ 30 ]. Among them, cell locomotion is easy to monitor to directly manifest the ciliary motility.…”

Section: Introductionmentioning

confidence: 99%

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

Suryanto

Vasquez

Roldan

et al. 2022

Cells

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Protozoa are eukaryotic, unicellular microorganisms that have an important ecological role, are easy to handle, and grow rapidly, which makes them suitable for ecotoxicity assessment. Previous methods for locomotion tracking in protozoa are largely based on software with the drawback of high cost and/or low operation throughput. This study aimed to develop an automated pipeline to measure the locomotion activity of the ciliated protozoan Tetrahymena thermophila using a machine learning-based software, TRex, to conduct tracking. Behavioral endpoints, including the total distance, velocity, burst movement, angular velocity, meandering, and rotation movement, were derived from the coordinates of individual cells. To validate the utility, we measured the locomotor activity in either the knockout mutant of the dynein subunit DYH7 or under starvation. Significant reduction of locomotion and alteration of behavior was detected in either the dynein mutant or in the starvation condition. We also analyzed how Tetrahymena locomotion was affected by the exposure to copper sulfate and showed that our method indeed can be used to conduct a toxicity assessment in a high-throughput manner. Finally, we performed a principal component analysis and hierarchy clustering to demonstrate that our analysis could potentially differentiate altered behaviors affected by different factors. Taken together, this study offers a robust methodology for Tetrahymena locomotion tracking in a high-throughput manner for the first time.

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Importance of protozoa Tetrahymena in toxicological studies: A review

Maurya

Pandey

2020

Science of The Total Environment

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Tetrahymena pyriformis Phagocytic Activity Test for Rapid Toxicity Assessment of Aquatic Micropollutants

Abstract: Abstract

Cited by 3 publications

References 43 publications

Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

Importance of protozoa Tetrahymena in toxicological studies: A review

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