Tetrandrine can alleviate inflammation and delay the growth of lung cancer during low-dose radiotherapy of non-small cell lung cancer

Abstract

This study investigated the expression of inflammatory factors in tumour tissues, monocytes and blood of non-small cell lung cancer (NSCLC) patients taking tetrandrine (Tet) during low-dose radiotherapy. Sixty NSCLC patients underwent surgical resection of lung cancer after low-dose radiotherapy. Thirty patients did not take Tet (control group) and the other thirty patients took Tet during radiotherapy (experimental group). Lung cancer tissues were collected from patients, and tumour-adjacent tissues were collected as controls. Peripheral blood was collected on the morning of radiotherapy for mononuclear cell separation. Quantitative real-time polymerase chain reaction was used to determine mRNA expression, and Western blotting was used to measure protein expression. Enzyme-linked immunosorbent assay was used to determine the inflammatory factor contents in liquid samples. Cell proliferation was determined via MTT assay at 24, 48 and 72 h after transfection in A549 cells. Tet reduced COX-2 mRNA in serum and blood monocytes and decreased COX-2 protein expression in monocytes from patients with radiotherapy. It decreased the release of inflammatory factors to blood after radiotherapy. Combined treatment with low-dose radiotherapy and Tet tablets reduced the expression of Ki-67 protein in tumour tissues, and decreased the release of inflammatory factors from NSCLC tissues after radiotherapy. Combined treatment with low-dose radiotherapy and Tet tablets reduced the proliferation of cancer cells possibly through reducing inflammatory factors released by tumour tissues. The study demonstrates that Tet administration during low-dose radiotherapy reduces the release of inflammatory factors by NSCLC tissues, and decreases tumour proliferation. Tet plays an auxiliary role in NSCLC radiotherapy.

Shedding light on ONOO− detection: the emergence of a fast-response fluorescent probe for biological systems

“…Inflammation is a physiological response of the body to stimuli such as injury or infection, which can cause tissue damage and disease development. 42 Detecting inflammatory tissue can provide information about the type, severity, and progression of the disease. This helps in early diagnosis and treatment of the disease, improving the treatment outcomes and prognosis.…”

Active Ingredients from Chinese Medicine for Combination Cancer Therapy

“…The application of AIFCM in this regard is merely in its initial stage, and exciting progress can be expected in the near future. Radiosensitization effect, inducing apoptosis via miR-34a/Sirt1/p53 signaling pathway; attenuating angiogenesis [189,193] Radiotherapy Coptis chinensis Berberine Inhibiting the expression of HIF-1 and VEGF, reversing radioresistance [190] Radiotherapy Magnolia Honokiol Suppressing the Notch signaling pathway, inhibiting radiation resistance and CSCs promotion [191] Radiotherapy Rhizoma zeodaria β-elemene Radiosensitization effect, suppressing the Prx-1/NF-kB /iNOS pathway, inhibiting EMT and CSC [192] Radiotherapy Radix stephania tetrandrae Tetrandrine Reducing the inflammatory factors, alleviating inflammation [194] Immunotherapy Rhizoma polygoni cuspidati Pigenin Down-regulation of p-EGFR, p-Akt, p-STAT3 and Cyclin D1, promotion of antibody-mediated cytotoxicity and apoptosis [207] Immunotherapy Garcinia hanburyi Gambogic acid Promoting antibody-mediated cytotoxicity [208] Immunotherapy Rhizoma polygoni cuspidati…”

“…In addition, Wu's group reported that EGCG could attenuate the induction of angiogenesis by gamma radiation in breast cancer patients within the combination of EGCG and ionizing radiation 193 . Moreover, Liang et al used tetrandrine to reduce the inflammatory factor release of A549 tumor tissue to alleviate inflammation and improve the tumor inhibition effect in radiotherapy 194 .…”

Sequential detection of inflammation-related hypochlorite ions and viscosity with a relay fluorescent probe

“…2 Inflammation can clear the original damage and necrotic cells and tissues damaged during the inflammatory process, initiating tissue repair. 3 Previous studies show that immune cells, blood vessels, and molecular mediators are closely related to the formation of inflammation, while reactive oxides and viscosity are closely related to cellular inflammation and the pathogenesis of certain diseases. 4 In organisms, H 2 O 2 and Cl − are taken by peroxidase catalyzed reactions to form hypochlorite ions (ClO − ).…”