The 24‐hour shelf‐life of cytapheresis platelet concentrates stored in polyvinyl chloride containers should be extended only with caution

Rg, Strauss; Snyder, Edward L.; Eckermann, I.; Stewart, Lauraine M.

doi:10.1046/j.1537-2995.1987.27387235642.x

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…Platelet response to hypotonic stress was measured by the described method [12,13] with some modifications. The platelet suspension (3x 108/ml) in plasma was treated at room temperature with half a volume of either distilled water or phosphate-buffered saline (pH 7.4).…”

Section: Function Metabolism and Morphology Testsmentioning

confidence: 99%

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

Shimizu¹,

Kouketsu²,

Kamiya³

et al. 1989

Vox Sanguinis

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A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22°C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 mm. The film was made of polymer blends consisting of polypropylene, styrene ethylene butylene styrene-block copolymer, and ehtylene ethylacrylate copolymer. The PO container with enough strength and flexibility in routine practice has 2 and 1.6 times higher oxygen and carbon dioxide gas transfer properties than standard polyvinyl chloride (PVC) plastic containers. PCs (1-1.9x10^11 platelets) processed from 450ml platelet-rich plasma were stored in 0.6-liter PO containers at 22°C with flatbed agitation for up to 6 days. The pH of PCs was well maintained at the mean values of 7.0 in PO containers after 6 days in the well-oxygenated condition. The energy metabolism of stored platelets was determined. Oxygen consumption rates of platelets stored in PO containers averaged 1.5 nmol/min/10^9 platelets. The rates of glucose consumption and lactate production were 0.4 and 0.8 nmol/min/10^11 platelets, respectively. The rates of adenosine triphosphate (ATP) generation of platelets, 9.7 nmol/min/109 platelets, in PO containers did not differ from those in the PVC containers. Aggregation responses to adenosine diphosphate and hypotonic shock response of platelets were better maintained in PO containers. The morphological changes into sphere forms with projections and the appearance of unclassified forms were more frequently observed in PO than in PVC containers. Single-donor apheresis platelet products collected by the PCS system may be stored in a new 0.6-liter PO container for over 5 days under acceptable conditions.

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Section: Function Metabolism and Morphology Testsmentioning

confidence: 99%

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

Shimizu¹,

Kouketsu²,

Kamiya³

et al. 1989

Vox Sanguinis

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“…The pH at 37°C of 5-day-stored 6 PCs was 7.1+0.1 in PVC/DEHP bags containing 0.8-1.2 X 1011 platelets and 7.0+0.1 in PVC/TOTM bags containing 0.8-1.4 X 1011 platelets. The shelf life of platelet products is determined by two major factors, the sterility and viabilioy of stored platelets (Strauss et al 1987). Bacteria were only occasionally detected even in the open system (Szymanski 1985).…”

mentioning

confidence: 99%

New closed system using a sterile connection device and preconnected PRP pack for extended storage of apheresis platelet products.

Shimizu

Kouketsu

Morishima

et al. 1988

Tohoku J. Exp. Med.

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In order to prevent bacterial contamination during the procedure of plateletapheresis, preconnected platelet-rich plasma (PRP) packs were prepared which include the apheresis bowl of a plasma collecting system (Haemonetics) with a 16G needle and a 0.6-and 1-liter double-bag system. The anticoagulant line of the PRP pack and that from the bag containing acid-citratedextrose formula A solution were welded by a sterile connection device (SCD model 312, DuPont). No additional care was needed to perform plateletapheresis with the present closed system. Platelet concentrates (PCs) stored in 1-liter bags made of polyvinyl chloride plastics with a plasticizer of either di (2-ethylhexyl) phthalate or tri (2-ethylhexyl) trimellitate were demonstrated to be sterile. The pH of PCs was maintained over 6.8 after 5-day storage. Preliminary studies suggest that the present closed system using the preconnected PRP pack and sterile connection device will be, useful for reducing the risk of bacterial contamination in platelet products during single-donor plateletapheresis.closed plateletapheresis system ; preconnected PRP pack ; sterile connection device ; platelet concentrates ; in vitro storage Recently, a unique sterile connection device (SCD, SCDTM model 312, DuPont, Wilmington, DE, USA) was developed to make a sterile, welded connection between two pieces of tubing through an application of the thermal welding process. Here we wish to introduce a new functionally closed system using such a device for collection of single donor platelet-rich plasma (PRP). Fig. l shows a disposable apheresis set of a plasma collecting system (PCS, Haemonetics Corp., Braintree, MA, USA), in which the apheresis bowl set was preconnected with a 16G needle and a double 0.6-and 1-liter polyvinyl chloride (PVC) plastic (plasticizer : di (2-ethylhexyl) phthalate, DEHP) bag system (Kawasumi Co., Ltd., Tokyo) for plasma and platelet preservation. A 0.5-liter of acid-citrate-dextrose formula A (ACD-A) solution was sterilized in PVC bags with preattached 20 cm tubing (Kawasumi Co.

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New version of heat-sealed LE-2® polyolefin container with improved gas permeability for storage of cytapheresis platelets

Shimizu¹,

Kouketsu²,

Kamiya³

et al. 1989

Transfusion Science

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The 24‐hour shelf‐life of cytapheresis platelet concentrates stored in polyvinyl chloride containers should be extended only with caution

Cited by 7 publications

References 9 publications

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

A Novel Second-Generation Polyolefin Container for Storage of Single-Donor Apheresis Platelets

New closed system using a sterile connection device and preconnected PRP pack for extended storage of apheresis platelet products.

New version of heat-sealed LE-2® polyolefin container with improved gas permeability for storage of cytapheresis platelets

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