The 30/35 kDa chymotryptic fragment of fibronectin enhances retroviral-mediated gene transfer in purified chronic myelogenous leukemia bone marrow progenitors

Traycoff, Christie M.; Srour, EF; Dutt, Parmesh; Fan, Yi; Cornetta, Kenneth

doi:10.1038/sj.leu.2400529

Cited by 13 publications

(10 citation statements)

References 5 publications

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“…Polycations such as Polybrene and protamine sulfate have long been known to increase gene transfer efficiency, presumably by facilitating interaction between the negatively charged vector particles and target cells (8,9,45). More recently, colocalization of oncoretroviral particles with target cells by using fibronectin has also been shown to increase gene transfer (33,46) by binding vector particles via a high-affinity heparin binding site and binding cells via VLA-4 and/or VLA-5 binding sites (20).…”

Section: Rrv and Sfv Glycoproteins Pseudotype Lentivirus Vectorsmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Kahl¹,

Marsh²,

Fyffe³

et al. 2004

J Virol

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Ross River virus (RRV) and Semliki Forest virus (SFV) are two alphaviruses that have a high degree of amino acid homology, as well as a very broad host range. We show here that envelope glycoproteins derived from both viruses can pseudotype human immunodeficiency virus type 1 (HIV-1)-derived lentivirus vectors. Both RRV and SFV glycoproteins considerably expand the host range of the lentivirus vector, and vectors can be efficiently concentrated by ultracentrifugation. A systematic analysis comparing the alphaviral glycoproteins to the vesicular stomatitis virus glycoprotein (VSV-G) revealed that lentivirus vectors incorporate RRV glycoproteins with an efficiency comparable to that of VSV-G. Both pseudotypes have comparable physical titers, but infectious titers with the RRV pseudotype are lower than with VSV-G. Incorporation of SFV glycoproteins into lentivirus vector is less efficient, leading to decreased physical and infectious titers. The transduction rates with VSV-G-, RRV-, and SFV-pseudotyped lentivirus vectors into adherent cell lines can be significantly increased by using a combination of Polybrene and plates coated with CH-296 recombinant fibronectin fragments. Together, our data suggest that RRV and SFV glycoproteins might be suitable as alternatives to VSV-G for pseudotyping lentivirus vectors.

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Section: Rrv and Sfv Glycoproteins Pseudotype Lentivirus Vectorsmentioning

confidence: 99%

Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Kahl¹,

Marsh²,

Fyffe³

et al. 2004

J Virol

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“…Acute GVHD was graded as 0-IV according to the criteria of Glucksberg et al 12 PCR for BCR/ABL was performed as previously described. 13 …”

Section: Assessment Of Gvhd Engraftment and Relapsementioning

confidence: 99%

Rapid engraftment after allogeneic transplantation using CD34-enriched marrow cells

Cornetta

Gharpure

Mills

et al. 1998

Bone Marrow Transplant

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Summary:Bone marrow cells expressing the surface antigen CD34 comprise approximately 1% of harvested marrow and are highly enriched for marrow progenitor cells, including the cells believed to be responsible for long-term engraftment following bone marrow transplantation (BMT). Selection of CD34-expressing cells was applied in allogeneic BMT (alloBMT) to decrease the number of T lymphocytes in the infused marrow in an attempt to prevent severe graft-versus-host disease (GVHD). We report 14 patients who underwent HLA-identical sibling-matched alloBMT with marrow-enriched for CD34 cells using the Isolex 300 SA device. Patients received total body irradiation, thiotepa, cyclophosphamide, antithymocyte globulin and methylprednisolone prior to marrow infusion. No post-transplantation immunosuppressive therapy was given except for a 5-week course of steroids. The purity of the infused marrow was 64.9 ؎ 6.0% (mean ؎ s.e.m.) CD34-positive cells and patients received a mean of 1.24 ؎ 0.21 ؋ 10 6 CD34 cells/kg. A mean of 9.4 ؎ 1.7 ؋ 10 4 CD3 T cells/kg were present in the CD34-enriched product, representing a 2.7 ؎ 0.1 log depletion. There were no graft rejections and patients achieved a sustained absolute granulocyte count of Ͼ500 in a median of 10.5 days and a sustained platelet engraftment of Ͼ20 000 untransfused in a median of 27 days. Patients were discharged a median of 21.5 days after marrow infusion. There were no instances of grade III or IV graft-versus-host disease (GVHD) and no unexpected adverse events during the transplant hospitalization. With a median follow-up of 12 months, the estimated 100 day survival is 86 ؎ 9%. CD34 selection in alloBMT permits rapid engraftment without unanticipated toxicities. Keywords: CD34 selection; engraftment; allogeneic transplantationThe marrow infused during bone marrow transplantation (BMT) contains a wide variety of cells, the vast majority

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“…In a subsequent publication, they demonstrated that co-localization of marrow cell and vector particles was the mechanism by which increased gene transfer was achieved [31]. This finding has been confirmed by our group, using normal and leukemic progenitors from patients with chronic myeloid leukemia [32]. Since a recombinant fibronectin fragment is now available, this new approach may significantly increase gene transfer rates, while maximizing the safety of the procedure [31].…”

Section: Overcoming Transduction Inefficiencymentioning

confidence: 59%

“…Chronic myeloid leukemia (CML) might be one exception because of the chimeric balance between normal and leukemic stem cells which can be manipulated, at least temporarily, by high-dose chemotherapy or interferon. Cell sorting of the autologous graft for CD34-positive HLA-DRnegative cells permits enrichment of normal primitive progenitors from CML [43][44], and once isolated, these normal progenitor cells are being evaluated as targets for drug-resistance genes [32,45]. We have shown that gene transfer into CD34-positive HLA-DR-negative progenitors can be increased approximately 10-fold using supernate transduction in the presence of the 30/35 kD fibronectin fragment [32].…”

Section: Drug Resistance Genes In Leukemia and Cancer Therapymentioning

confidence: 99%

“…Cell sorting of the autologous graft for CD34-positive HLA-DRnegative cells permits enrichment of normal primitive progenitors from CML [43][44], and once isolated, these normal progenitor cells are being evaluated as targets for drug-resistance genes [32,45]. We have shown that gene transfer into CD34-positive HLA-DR-negative progenitors can be increased approximately 10-fold using supernate transduction in the presence of the 30/35 kD fibronectin fragment [32]. Unfortunately, a small number of leukemic progenitors can contaminate the CD34-positive HLA-DR-negative sorted population, and until ''cleaner'' preparations of non-leukemic progenitors are obtained, there is significant risk of introducing the drug resistance gene into leukemic progenitors [32].…”

Section: Drug Resistance Genes In Leukemia and Cancer Therapymentioning

confidence: 99%

See 1 more Smart Citation

Retroviral gene therapy in hematopoietic diseases

Cornetta

Fan

1997

J. Clin. Apheresis

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The 30/35 kDa chymotryptic fragment of fibronectin enhances retroviral-mediated gene transfer in purified chronic myelogenous leukemia bone marrow progenitors

Abstract: HLA-DR − cells, a population of BM cells known to be enriched for normal primitive HSC 11-13 and non-leukemic

Cited by 13 publications

References 5 publications

Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Rapid engraftment after allogeneic transplantation using CD34-enriched marrow cells

Retroviral gene therapy in hematopoietic diseases

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