The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum

Marmey, Philippe; Jalloul, Ai͏̈da; Alhamdia, Majd; Assigbétsé, Komi; Cacas, Jean‐Luc; Voloudakis, Andreas E.; Champion, Antony; Clérivet, Alain; Montillet, Jean‐Luc; Nicole, Michel

doi:10.1016/j.plaphy.2007.05.002

Cited by 43 publications

(58 citation statements)

References 51 publications

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“…Interestingly, HR-like symptoms were observed in cotton when a compatible Xanthomonas campestris pv. malvacearum strain was inoculated onto MeJA-treated cotyledons (27). This may indicate the potential of compatible interactions to trigger PCD responses provided that certain threshold levels of oxylipins accumulate during infection.…”

Section: Discussionmentioning

confidence: 99%

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Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

García-Marcos

Pacheco

Manzano

et al. 2013

J Virol

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One of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. Although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. Here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis response caused either by the synergistic interaction of Potato virus X with Potato virus Y (PVX-PVY) or by Tomato spotted wilt virus (TSWV) in Nicotiana benthamiana. Silencing either 9-lipoxygenase (LOX), 13-LOX, or ␣-dioxygenase-1 (␣-DOX-1) attenuated the programmed cell death (PCD)-associated symptoms caused by infection with either PVX-PVY or TSWV. In contrast, silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1), expedited cell death during infection with compatible viruses. This correlated with an enhanced expression of oxylipin biosynthesis genes and dioxygenase activity in PVX-PVY-infected plants. Moreover, the Arabidopsis thaliana double lox1 ␣-dox-1 mutant became less susceptible to TSWV infection. We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta.

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Section: Discussionmentioning

confidence: 99%

“…In addition, JA has been implicated in the signaling cascade leading to LOX elicitation. Methyl jasmonate (MeJA) treatment was reported to induce LOX activities and the transcription of the corresponding genes in plants (24,27). Little information is available about the role of oxylipins in virus-infected plants.…”

mentioning

confidence: 99%

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

García-Marcos

Pacheco

Manzano

et al. 2013

J Virol

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“…7). Both genes have been shown to regulate late anther and pollen development via JA signaling (Marmey et al, 2007;Li et al, 2013). Moreover, the transgenic stamen also was characterized with decreased expression of the auxin biosynthesis genes GhTAA1 and GhYUC5, the auxin signaling receptor gene GhABP1, and the auxinresponsive factor GhARF2 (Fig.…”

Section: Vin Contributes To Male Fertility Probably By Impacting Starmentioning

confidence: 98%

“…FW, Fresh weight. pollen viability (Ishiguro et al, 2001;Scott et al, 2004), and hexose signaling is involved in JA biosynthesis and signaling (Hamann et al, 2009). In cotton, a pollen-specific R2-R3 MYB gene, GhMYB24, and a 9-lipoxygenase gene, GhLOX1, have been identified to regulate late anther and pollen development in response to JA signaling (Marmey et al, 2007;Li et al, 2013). The GhMYB24 transcript level was reduced by 59%, 31%, and 34% of that in the wild type in the 21-d stamens of the RNAi lines 15-4-2, 28-4-1, and 61-9-1, respectively, with the GhLOX1 mRNA level reduced by approximately 90% in line 15-4-1 and by approximately 80% in the remaining two lines (Fig.…”

Section: Rnai-mediated Suppression Of Ghvin Expression Led To a Signimentioning

confidence: 99%

Critical Roles of Vacuolar Invertase in Floral Organ Development and Male and Female Fertilities Are Revealed through Characterization of GhVIN1-RNAi Cotton Plants

Wang

Ruan

2016

Plant Physiol.

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Seed number and quality are key traits determining plant fitness and crop yield and rely on combined competence in male and female fertilities. Sucrose metabolism is central to reproductive success. It remains elusive, though, how individual sucrose metabolic enzymes may regulate the complex reproductive processes. Here, by silencing vacuolar invertase (VIN) genes in cotton (Gossypium hirsutum) reproductive organs, we revealed diverse roles that VIN plays in multiple reproductive processes. A set of phenotypic and genetic studies showed significant reductions of viable seeds in GhVIN1-RNAi plants, attributed to pollination failure and impaired male and female fertilities. The former was largely owing to the spatial mismatch between style and stamen and delayed pollen release from the anthers, whereas male defects came from poor pollen viability. The transgenic stamen exhibited altered expression of the genes responsible for starch metabolism and auxin and jasmonic acid signaling. Further analyses identified the reduction of GhVIN expression in the seed coat as the major cause for the reduced female fertility, which appeared to disrupt the expression of some key genes involved in trehalose and auxin metabolism and signaling, leading to programmed cell death or growth repression in the filial tissues. Together, the data provide an unprecedented example of how VIN is required to synchronize style and stamen development and the formation of male and female fertilities for seed development in a crop species, cotton.

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“…Activation of LOXs in plants may be involved in cell death induced by pathogens (Buonaurio and Servili, 1999;Rusterucci et al, 1999). The induction of HR-like cell death by the activation of the 9-LOX-encoding gene GhLOX1 was shown in cotton (Gossypium hirsutum) plants during Xanthomonas campestris pv malvacearum infection (Marmey et al, 2007). LOX activity increases in parallel with the induction of HR symptoms in tobacco; however, in compatible interactions, LOX activity is delayed and reaches much lower levels .…”

mentioning

confidence: 99%

The Pepper 9-Lipoxygenase GeneCaLOX1Functions in Defense and Cell Death Responses to Microbial Pathogens

2009

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Lipoxygenases (LOXs) are crucial for lipid peroxidation processes during plant defense responses to pathogen infection. A pepper (Capsicum annuum) 9-LOX gene, CaLOX1, which encodes a 9-specific lipoxygenase, was isolated from pepper leaves. Recombinant CaLOX1 protein expressed in Escherichia coli catalyzed the hydroperoxidation of linoleic acid, with a K m value of 113. 9 mM. Expression of CaLOX1 was differentially induced in pepper leaves not only during Xanthomonas campestris pv vesicatoria (Xcv) infection but also after exposure to abiotic elicitors. Transient expression of CaLOX1 in pepper leaves induced the cell death phenotype and defense responses. CaLOX1-silenced pepper plants were more susceptible to Xcv and Colletotrichum coccodes infection, which was accompanied by reduced expression of defense-related genes, lowered lipid peroxidation, as well as decreased reactive oxygen species and lowered salicylic acid accumulation. Infection with Xcv, especially in an incompatible interaction, rapidly stimulated LOX activity in unsilenced, but not CaLOX1-silenced, pepper leaves. Furthermore, overexpression of CaLOX1 in Arabidopsis (Arabidopsis thaliana) conferred enhanced resistance to Pseudomonas syringae pv tomato, Hyaloperonospora arabidopsidis, and Alternaria brassicicola. In contrast, mutation of the Arabidopsis CaLOX1 ortholog AtLOX1 significantly increased susceptibility to these three pathogens. Together, these results suggest that CaLOX1 and AtLOX1 positively regulate defense and cell death responses to microbial pathogens.

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The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum

Cited by 43 publications

References 51 publications

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

Critical Roles of Vacuolar Invertase in Floral Organ Development and Male and Female Fertilities Are Revealed through Characterization of GhVIN1-RNAi Cotton Plants

The Pepper 9-Lipoxygenase GeneCaLOX1Functions in Defense and Cell Death Responses to Microbial Pathogens

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