The ABA-Deficiency Suppressor Locus HAS2 Encodes the PPR Protein LOI1/MEF11 Involved in Mitochondrial RNA Editing

Sechet, Julien; Roux, Camille; Plessis, Anne; Effroy, Delphine; Frey, Anne; Perreau, François; Biniek, Catherine; Krieger‐Liszkay, Anja; Macherel, David; North, Helen; Mireau, Hakim; Marion‐Poll, Annie

doi:10.1016/j.molp.2014.12.005

Cited by 37 publications

(30 citation statements)

References 75 publications

(135 reference statements)

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“…For a final volume of 200 μl, SNP (0, 10, 25, 50, 75, and 100 μM) was mixed with 5 μM DAF‐FM in 10 mM Tris–HCl at pH 7.4 and incubated at 25°C (Figure S1). The experiments were conducted in darkness to avoid degradation of the light‐sensitive DAF‐FM (Sechet et al, ). The emission of NO by sodium nitroprusside is optimal under light, but NO is also produced from sodium nitroprusside in the dark (Shishido & de Oliveira, ).…”

Section: Methodsmentioning

confidence: 99%

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Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Nagel

Alqudah

Bailly

et al. 2019

Plant Cell & Environment

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Barley is used for food and feed, and brewing. Nondormant seeds are required for malting, but the lack of dormancy can lead to preharvest sprouting (PHS), which is also undesired. Here, we report several new loci that modulate barley seed dormancy and PHS. Using genome‐wide association mapping of 184 spring barley genotypes, we identified four new, highly significant associations on chromosomes 1H, 3H, and 5H previously not associated with barley seed dormancy or PHS. A total of 71 responsible genes were found mostly related to flowering time and hormone signalling. A homolog of the well‐known Arabidopsis Delay of Germination 1 (DOG1) gene was annotated on the barley chromosome 3H. Unexpectedly, DOG1 appears to play only a minor role in barley seed dormancy. However, the gibberellin oxidase gene HvGA20ox1 contributed to dormancy alleviation, and another seven important loci changed significantly during after‐ripening. Furthermore, nitric oxide release correlated negatively with dormancy and shared 27 associations. Origin and growth environment affected seed dormancy and PHS more than did agronomic traits. Days to anthesis and maturity were shorter when seeds were produced under drier conditions, seeds were less dormant, and PHS increased, with a heritability of 0.57–0.80. The results are expected to be useful for crop improvement.

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Section: Methodsmentioning

confidence: 99%

“…The experiments were conducted in darkness to avoid degradation of the light-sensitive DAF-FM (Sechet et al, 2015). The emission of NO by sodium nitroprusside is optimal under light, but NO is also produced from sodium nitroprusside in the dark (Shishido & de Oliveira, 2001).…”

Section: Multiplication and Phenotypingmentioning

confidence: 99%

Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Nagel

Alqudah

Bailly

et al. 2019

Plant Cell & Environment

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“…Genome sequencing was carried out on the has1 aba3-1 mutant after two backcrosses to the aba3-1 mutant. DNA was isolated as described by Sechet et al (2015), and sequencing was performed by TGAC using an Illumina HiSeq 2000 sequencer.…”

Section: Map-based Cloning and Sequencingmentioning

confidence: 99%

Clathrin Heavy Chain Subunits Coordinate Endo- and Exocytic Traffic and Affect Stomatal Movement

et al. 2017

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The current model for vesicular traffic to and from the plasma membrane is accepted, but the molecular requirements for this coordination are not well defined. We have identified the hot ABA-deficiency suppressor1 mutant, which has a stomatal function defect, as a clathrin heavy chain1 (CHC1) mutant allele and show that it has a decreased rate of endocytosis and growth defects that are shared with other chc1 mutant alleles. We used chc1 alleles and the related chc2 mutant as tools to investigate the effects that clathrin defects have on secretion pathways and plant growth. We show that secretion and endocytosis at the plasma membrane are sensitive to CHC1 and CHC2 function in seedling roots and that chc mutants have physiological defects in stomatal function and plant growth that have not been described previously. These findings suggest that clathrin supports specific functions in multiple cell types. Stomata movement and gas exchange are altered in chc mutants, indicating that clathrin is important for stomatal regulation. The aberrant function of chc mutant stomata is consistent with the growth phenotypes observed under different water and light conditions, which also are similar to those of the secretory SNARE mutant, syp121. The syp121 and chc mutants have impaired endocytosis and exocytosis compared with the wild type, indicating a link between SYP121-dependent secretion and clathrin-dependent endocytosis at the plasma membrane. Our findings provide evidence that clathrin and SYP121 functions are important for the coordination of endocytosis and exocytosis and have an impact on stomatal function, gas exchange, and vegetative growth in Arabidopsis (Arabidopsis thaliana).

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“…napus 29 . GRMZM2G053384 is a homolog of AtLOI1 , which encodes a PPR protein involved in drought stress tolerance in Arabidopsis 30 . GRMZM2G470984 encodes a phytosulfokine (PSK).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, homologs for 19 candidate genes in Arabidopsis or rice (Supplemental Tables S1 and S2) have been reported to be associated with tolerance to other abiotic stresses. For example, in Arabidopsis , AtLOI1 (a GRMZM2G053384 homolog), AtSec20 (a GRMZM2G457267 homolog), and AtTIP2;3 (a GRMZM2G027098 homolog) were found to be involved in osmotic or drought tolerance 30, 33, 40 , while AtCLC-C (a GRMZM2G332258 homolog) and UBP16 (a GRMZM2G000404 homolog) were associated with salt tolerance 34, 48 . However, no locus or candidate gene is identical to that revealed by GWAS with maize inbred lines in previous studies 4, 5, 14 .…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping with testcrossing associations and F2:3 populations reveals the importance of heterosis in chilling tolerance at maize seedling stage

Jin-bo

et al. 2017

Sci Rep

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Maize seedlings are sensitive to low temperatures, and genetic mapping for chilling tolerance at the seedling stage with genetically diverse populations would facilitate the genetic improvement of this important trait. In this study, quantitative trait loci (QTL) mapping for four chilling tolerancerelated traits at the seedling stage was conducted via a genome-wide association study (GWAS) with 338 testcrosses. A total of 32 significant loci and 36 stress tolerance-related candidate genes were identified, though none of them have been revealed by QTL mapping using maize inbred lines in previous reports. Moreover, expression of ten of the candidate genes was induced by chilling stress in a maize hybrid, though only a few of these genes were upregulated in its tolerant parent. These implied that heterosis might be involved in maize chilling tolerance. To further evaluate the importance of heterosis in chilling tolerance at the seedling stage, genetic mapping for chilling tolerance was conducted using an F 2:3 population derived from the two inbred lines used for the gene expression assay. Of the seven QTL revealed, six loci showed partial dominance or over-dominance effects. Results from this study demonstrate that heterosis plays an important role in chilling tolerance in maize seedlings.Maize (Zea mays L.) is an important food, energy, forage and industrial crop. However, chilling (0-15 °C) has become a major environmental factor that limits maize production and its distribution. Chilling stress affects germination, seedling growth, early leaf development and overall maize crop growth and productivity 1 . In particular, maize seedlings are the most sensitive to chilling stress during the transition phase from heterotrophic to autotrophic growth 2 . Therefore, elucidating the mechanism of maize chilling tolerance at the seedling stage (3-leaf stage) will help to genetically improve this trait.Yield performance is usually used to evaluate stress tolerance at the reproductive stage, while at the seedling stage, it mainly relies on morphological-physiological traits. Physiological traits, such as photosynthetic performance 3-5 , tissue water content, and levels of abscisic acid (ABA) and antioxidants 3 , usually respond to low temperatures earlier than changes in morphological traits and are extensively used as indicators of chilling tolerance. Additionally, as a kind of compatible solute to increase the osmotic potential and stabilize macromolecular structure, soluble sugar content can also be a good index for stress tolerance evaluation 6 . At the morphological level, chilling stress causes decreased growth rates, leaf elongation and dry weights 2 . Chilling stress decreases root hydraulic conductance 7 and often results in water-stress symptoms. Root development in chilling-sensitive maize seedlings was found to be distinctly reduced under chilling stress, and the root-to-shoot ratio in chilling-sensitive inbred plants decreased under chilling stress conditions at the 2-to 3-leaf stage 8 . Because physiological t...

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The ABA-Deficiency Suppressor Locus HAS2 Encodes the PPR Protein LOI1/MEF11 Involved in Mitochondrial RNA Editing

Cited by 37 publications

References 75 publications

Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Novel loci and a role for nitric oxide for seed dormancy and preharvest sprouting in barley

Clathrin Heavy Chain Subunits Coordinate Endo- and Exocytic Traffic and Affect Stomatal Movement

Genetic mapping with testcrossing associations and F2:3 populations reveals the importance of heterosis in chilling tolerance at maize seedling stage

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