The ABC transporter SpTUR2 confers resistance to the antifungal diterpene sclareol

Abstract

SummaryPDR5-like proteins represent one group of the ABC superfamily of transporters. Members of this group are present in plants and, due to the function of PDR5-related proteins in fungi in the excretion of xenobiotics (including antifungal agents), it has been proposed that they might play a similar role in plants in the response to and detoxi®cation of herbicides and fungicides. However, until now no functional data has been presented showing an altered plant response to any herbicide or fungicide as a result of manipulating the expression of a PDR5-like gene in plants. In this paper, we show that the plant SpTUR2 PDR5-like ABC transporter is localised to the plasma membrane and that expression of this protein in Arabidopsis leads to the acquisition of resistance to the diterpenoid antifungal agent sclareol. These data both de®ne a possible endogenous substrate for this transporter and highlight the potential of manipulating plant chemical resistance via modulating the expression of speci®c PDR5-like transporters.

Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

“…At least in a qualitative manner, the MetArray expression patterns matched most previously reported data by Northern, RT-PCR, or promoterreporter analyses of single CYP genes (Bell-Lelong et al, 1997;Mizutani et al, 1998), of genes encoding UGTs and glutathione-dependent enzymes (Warnecke et al, 1997;Jackson et al, 2001;Jones et al, 2003;Loutre et al, 2003;Rodriguez Milla et al, 2003), or groups of ABC transporter genes van den Bruˆle and Smart, 2002). A compilation of examples are provided as supplementary information (Supplementary Table 2).…”

“…In addition to CYP81D8, UGT73C5, and UGT87A2, which were not known to be stress-related, this group also contained the stress-responsive genes GSTF2, GSTF6, GSTF7 and PDR8 (Table 1; Figure 4). PDR-type ABC transporters might be involved in defense and detoxification, which had been indicated for three homologous members from other plant species (Smart and Fleming, 1996;Jasinski et al, 2001;Sasabe et al, 2002;van den Bruˆle et al, 2002). The known induction of the Arabidopsis ABC transporter MRP3 by sulfonylureas (Tommasini et al, 1997) was confirmed by this analysis.…”

“…The stresses included exposure to ozone, hydrogen peroxide, signaling molecules, heavy metals, heat shock, dehydration, wounding, biotic elicitors and microbial infection (Marrs, 1996;Mullineaux et al, 1998;Jackson et al, 2001Jackson et al, , 2002Sa´nchez-Ferna´ndez et al, 2001;Kolukisaoglu et al, 2002;Mazel and Levine, 2002; The highly homologous GSTF6 and GSTF7 may crosshybridize, however, independent analyses showed that both genes are responsive to primisulfuron (Table 4). UGT76E3 UGT76E10P UGT76E6 UGT76E4 UGT76E5 UGT76E11 UGT76E12 UGT76E7 UGT76E8P UGT76E9 UGT83A1 UGT74B1 UGT74F1 UGT74F2 UGT74C1 UGT74D1 UGT74E1 UGT74E2 UGT75C1 UGT75D1 UGT75B1 UGT75B2 UGT84B1 UGT84B3 UGT84B2 UGT84A1 UGT84A2 UGT84A3 UGT84A4 UGT85A4 UGT85A5 UGT85A2 UGT85A1 UGT85A6P UGT85A7 UGT86A1 UGT86A2 UGT87A1 UGT87A2 UGT92A1 UGT73D1 UGT73C7 UGT73C1 UGT73C2 UGT73C3 UGT73C4 UGT73C5 UGT73C6 UGT73B1 UGT73B2 UGT73B3 UGT73B4 UGT73B5 UGT89A1P UGT89A2 UGT89B1 UGT89C1 UGT90A1 UGT90A2 UGT79B1 UGT79B6 UGT79B9 UGT79B10 UGT79B11 UGT79B7 UGT79B8 UGT79B4 UGT79B5 UGT79B2 UGT79B3 UGT91C1 UGT91A1 UGT91B1 UGT80A2 van den Bruˆle and Smart, 2002;Wagner et al, 2002;Werck-Reichhart et al, 2002...…”

An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice

“…Spirodella polyrhiza SpTUR2 and rice OsPDR9 both play a role in the response to abiotic stress (44,45). ABCG full transporter genes also have been identified as being involved in volatile compound production and rhizosphere signaling (46,47).…”

Gene Trapping with Firefly Luciferase in Arabidopsis. Tagging of Stress-Responsive Genes

“…ABC transporters play an important role in detoxification and excretion of potentially harmful compounds in eukaryotes (Henikoff et al, 1997;Higgins, 2001). Plant ABC transporters are implicated in ion channel activity, detoxification, chlorophyll biosynthesis, stomatal movement, and various aspects of plant development (Sidler et al, 1998;Gaedeke et al, 2001;Kushnir et al, 2001;see: Martinoia et al, 2002;van den Brule et al, 2002). Transcript levels of ABC transporter genes are often enhanced by chemicals that are substrates for the transporters themselves; therefore, data on expression regulation can indicate their biochemical function (del Sorbo et al, 1997;Higgins, 2001).…”