The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development.

Parcy, François; Valon, Christiane; Kohara, Atuko; Miséra, Simon; Giraudat, Jérôme

doi:10.1105/tpc.9.8.1265

Cited by 260 publications

(94 citation statements)

References 34 publications

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“…Previous genetic data suggest that the transcription factors FUS3 and ABI3 act in concert to regulate key pathways during late embryogenesis and seed maturation (Ba¨umlein et al 1994;Parcy et al 1997;Raz et al 2001). Transactivation and binding studies demonstrated that the FUS3 factor physically interacts with the RY element of seed-specific gene promoters (Reidt et al 2000).…”

Section: Discussionmentioning

confidence: 95%

“…the VP1 gene in maize (McCarty et al 1991;McCarty 1995) and the genes ABI3 (abscisic acid insensitive 3), FUS3 (FUSCA3), LEC1 (leafy cotyledon 1) and LEC2 in Arabidopsis thaliana (Mu¨ller 1963;Koorneef et al 1994;Meinke et al 1994;West et al 1994). The relationships between ABI3, LEC1 and FUS3 have been studied in detail by investigating the phenotypes of double mutants (Ba¨umlein et al 1994;Meinke et al 1994;Parcy et al 1997). The combination of abi3 with lec1 or fus3 leads to highly pigmented and extremely viviparous embryos with a dramatically reduced protein content (Raz et al 2001).…”

Section: Introductionmentioning

confidence: 98%

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Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Mönke

Altschmied

Tewes

et al. 2004

Planta

165

141

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In Arabidopsis thaliana (L.) Heynh. the seed-specific transcription factors ABI3 and FUS3 have key regulatory functions during the development of mature seeds. The highly conserved RY motif [DNA motif CATGCA(TG)], present in many seed-specific promoters, is an essential target of both regulators. Here we show that, in vitro, the full-length ABI3 protein, as well as FUS3 protein, is able to bind to RY-DNA and that the B3 domains of both transcription factors are necessary and sufficient for the specific interaction with the RY element. Flanking sequences of the RY motif modulate the binding, but the presence of an RY sequence alone allows the specific interaction of ABI3 and FUS3 with the target in vitro. Transcriptional activity of ABI3 and FUS3, measured by transient promoter activation, requires the B3 DNA-binding domain and an activation domain. In addition to the known N-terminal-located activation domain, a second transcription activation domain was found in the B1 region of ABI3.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Mönke

Altschmied

Tewes

et al. 2004

Planta

165

141

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“…A number of other Arabidopsis mutants, including fus3 and lec1, that do not accumulate triacylglycerols in their seeds to the same extent as the wild type have also been isolated. But FUS3 and LEC1 presumably encode more general regulators of late embryo development rather than factors speciWcally governing triacylglycerol biosynthesis (Parcy et al 1997). Genomic and proteomic approaches will be needed to better understand the underlying metabolism of oilseeds and the regulatory networks that determine the quality and quantity of oils produced (Ohlrogge and Jaworski 1997;Slabas et al 2001;Ruuska et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed genes in seeds of two near-isogenic Brassica napus lines with different oil content

Wang

Han

et al. 2006

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The regulation of seed oil synthesis in rapeseed is largely unknown. In this study, we compared the gene expression during seed development between two lines of Brassica napus with a 10% difference in oil content. We isolated the immature seeds 15 and 25 days after flowering at periods preceding and including the major accumulation of storage oils and proteins. The differentially expressed gene clones between the two rape lines were isolated by subtractive suppression hybridization (SSH). All SSH clones were arrayed and screened by dot blot hybridization, followed by RT-PCR analysis for selected clones. A total of 217 cDNA clones corresponding to 30 genes were found to have a high expression in seeds with high oil content. Six genes were highly expressed in seeds with low oil content. Northern blot and enzyme activity analysis demonstrated a change in expression pattern of several genes. The results provide information on gene-encoding factors responsible for the regulation of oil synthesis. The possible role of these genes in seeds is discussed. The genes in this study may be suitable as novel targets for genetic improvement of seed oil content and may also provide molecular markers for studies of rape breeding.

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“…We also checked seed germination because Chl retention in mature seeds causes a decrease in germination rate (Parcy et al 1997;Nakajima et al 2012). As reported, the germination rate of nye1-1 mutants was significantly lower than WT, but the germination rate of nac016-1 seeds was almost the same as WT (Fig.…”

Section: Nac016 Does Not Regulate Sgr1 Transcription During Seed Degrmentioning

confidence: 86%

Arabidopsis NAC016 promotes chlorophyll breakdown by directly upregulating STAYGREEN1 transcription

et al. 2015

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The Arabidopsis transcriptional factor NAC016 directly activates chlorophyll degradation during leaf senescence by binding to the promoter of SGR1 and upregulating its transcription. During leaf senescence or abiotic stress in Arabidopsis thaliana, STAYGREEN1 (SGR1) promotes chlorophyll (Chl) degradation, acting with Chl catabolic enzymes, but the mechanism regulating SGR1 transcription remains largely unknown. Here, we show that the Arabidopsis senescence-associated NAC transcription factor NAC016 directly activates SGR1 transcription. Under senescence-promoting conditions, the expression of SGR1 was downregulated in nac016-1 mutants and upregulated in NAC016-overexpressing (NAC016-OX) plants. By yeast one-hybrid and chromatin immunoprecipitation assays, we found that NAC016 directly binds to the SGR1 promoter, which contains the NAC016-specific binding motif (termed the NAC016BM). Furthermore, nac016-1 SGR1-OX plants showed an early leaf yellowing phenotype, similar to SGR1-OX plants, confirming that NAC016 directly activates SGR1 expression in the leaf senescence regulatory cascade. Although we found that NAC016 activates SGR1 expression in senescing leaves, this transcriptional regulation is considerably weaker in maturing seeds; the seeds of sgr1-1 mutants (also known as nonyellowing1-1, nye1-1) stayed green, while the seeds of nac016-1 mutants turned from green to yellow normally. We also found that the abscisic acid (ABA) signaling-related transcription factor genes ABI5 and EEL and the ABA biosynthesis gene AAO3, which activate SGR1 expression directly or indirectly, were significantly downregulated in nac016-1 mutants and upregulated in NAC016-OX plants. However, the NAC016BM does not exist in their promoter regions, indicating that NAC016 may indirectly activate these ABA signaling and biosynthesis genes, probably by directly activating transcriptional cascades regulated by the NAC transcription factor NAP. The NAC016-mediated regulatory cascades of SGR1 and other Chl degradation-related genes are discussed. and chromatin immunoprecipitation assays, we found that NAC016 directly binds to the SGR1 21 promoter, which contains the NAC016-specific binding motif (termed the NAC016BM). 22Furthermore, nac016-1 SGR1-OX plants showed an early leaf yellowing phenotype, similar to 23 SGR1-OX plants, confirming that NAC016 directly activates SGR1 expression in the leaf 24 senescence regulatory cascade. Although we found that NAC016 activates SGR1 expression in 25 senescing leaves, this transcriptional regulation is considerably weaker in maturing seeds; the 26 seeds of sgr1-1 mutants (also known as nonyellowing1-1, nye1-1) stayed green, while the seeds of 27 nac016-1 mutants turned from green to yellow normally. We also found that the abscisic acid (ABA) 28 signaling-related transcription factor genes ABI5 and EEL and the ABA biosynthesis gene AAO3, 29 which activate SGR1 expression directly or indirectly, were significantly downregulated in nac016-1 30 mutants and upregulated in NAC016-OX plants. Howeve...

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The ABSCISIC ACID-INSENSITIVE3, FUSCA3, and LEAFY COTYLEDON1 loci act in concert to control multiple aspects of Arabidopsis seed development.

Cited by 260 publications

References 34 publications

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA

Identification of differentially expressed genes in seeds of two near-isogenic Brassica napus lines with different oil content

Arabidopsis NAC016 promotes chlorophyll breakdown by directly upregulating STAYGREEN1 transcription

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