The acceleration of garlic (Allium sativum L) ethanolic extract on gingival wound healing process in Wistar rats

Abstract

Garlic (Allium sativum L) is a medicinal plant traditionally used to relieve pain. Garlic's active constituents, allicin and triacremonone, have been proven to have antibacterial and antiinflammatory activity. The aim of this study was to investigate the effect of garlic ethanolic extract gel in gingival wound healing process of rats. Thirty male Wistar rats aged 10 weeks with body weight 200-250 g were subjected in this study. Rats were divided randomly into five groups with six rats in each group. Group I as negative control was given sodium carboxymethyl cellulose (Na CMC) base gel. Group II as positive control was given Benzydamine ® gel and Group IV-V were given garlic ethanolic extract gel at dose of 20, 40 and 80%, respectively. Each group was subdivided into two sub groups of three rats according to the decapitation period which were 5 th (D-5) and 7 th (D-7) day after the garlic extract gel application. Excisional wounds using punch biopsy, 2.5 mm in diameter, were created at the mandibular labial gingiva between right and left incisor teeth of the rats. The garlic extract gel of each preparation dose was then applied on the wound three times a day, starting at 0 day until 7 th day. The decapitation was conducted on the D-5 and D-7. Histological slides of wounded tissue were prepared. Epithelial thickness, new blood vessel, and number of fibroblast were examined. The results showed that the epithelial thickness of garlic ethanolic extract gel groups was significantly higher than control group (p<0.05), especially after 5 th day application. However, the number of new blood vessels and the amount of fibroblast of those groups were not significantly higher than control group (p>0.05). In conclusion, topical application of garlic ethanolic extract gel accelerates the gingival wound healing process in rats by increasing epithelial thickness. ABSTRAKBawang putih (Allium sativum L) merupakan tanaman obat yang secara tradisional digunakan untuk meredakan nyeri. Kandungan aktif bawang putih yaitu alisin dan triakremonon terbukti mempunyai aktivitas antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk mengkaji efek gel ekstrak etanol bawah putih pada proses penyembuhan luka gingiva tikus. Tiga puluh tikus jantan Wistar berumur 10 minggu dengan berat badan 200-250 g digunakan dalam penelitian ini. Tikus dibagi menjadi lima kelompok secara acak dengan enam ekor setiap kelompok. Kelompok I sebagai kontrol diberi natrium karboksilmetil selulosa (Na CMC). Kelompok II sebagai kontrol positif diberi gel Benzydamine ® . Kelompok III-IV diberi gel ektrak etanol bawang putih berturutturut pada dosis 20, 40 dan 80%. Masing kelompok selanjutnya dibagi dalam dua sub kelompok masing-masing tiga ekor tikus menurut waktu dekapitasi yaitu hari ke 5 (H-5) dan 7 (H-7) setelah pemberian gel ekstrak bawah putih. Luka eksisi dengan puch biopsy berdiameter 2,5 mm dibuat pada gingiva labial rahang bawah, di antara gigi incisivus kanan dan kiri tikus. Gel ekstrak bawang putih untuk setiap dosis sediaan dioleskan pada lu...

Chitosan based ethanolic Allium Sativum extract hydrogel film: a novel skin tissue regeneration platform for 2nd degree burn wound healing

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