The Accumulation of Phenolic Acids in Tissue Culture Pathogen Combinations of Solanum tuberosum and Phytophthora infestans

Robertson, N. F.; Friend, J.; Aveyard, Muriel A.; Brown, Jean; Huffee, Margaret; Homans, A. L.

doi:10.1099/00221287-54-2-261

Cited by 29 publications

(10 citation statements)

References 14 publications

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“…After continued growth and the establishment of fastgrowing callus, the capacity of pea callus to synthesize pisatin decreased. A similar phenomenon was reported by Robertson et al (1968). Ingram (1967) showed that, when a suspension of potato tuber callus was infected with Phytophthora infestans…”

Section: Discussionsupporting

confidence: 81%

“…Ingram &Robertson (1965) andIngram (1967) showed that callus cultures from potato tubers exhibited the same R-gene resistance to Phytophthora infestans as the intact tubers from which they were derived. Robertson et al (1968) demonstrated that this inhibition was due to phenolic acids produced after infection. However, Tomiyama et al (1968) implicated the phytoalexin rishitin in R-gene resistance of potato tubers.…”

Section: Introductionmentioning

confidence: 98%

“…In the initial work this toxicity was complete within 6 days. However, after continued growth of the callus for 3 years, toxicity was achieved only after 10 days (Robertson et al 1968). By careful selection one culture (pea root C) was obtained which continued to produce pisatin at high concentrations.…”

mentioning

confidence: 99%

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Pisatin Production by Tissue Cultures of Pisum sativum L.

Bailey

1970

Journal of General Microbiology

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SUMMARYCallus tissues of Pisum sativum produced the phytoalexin pisatin when grown in axenic culture. Coconut milk, which was in the culture media, induced pisatin formation in pea leaf discs. Cultures which had been established for over 18 months showed a marked reduction in the ability to produce pisatin. Pisatin inhibited the growth of pea callus at low concentrations (5 to 10 ,ug./ml.), and may control callus tissue initiation and cellular necrosis during host-fungus interactions.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 98%

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Pisatin Production by Tissue Cultures of Pisum sativum L.

Bailey

1970

Journal of General Microbiology

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“…The occurrence of free salicylic, p-OH-benzoic and vanillic acids in yellow nutsedge leaves can be associated with toxic and inhibitory effects (Robertson et al, 1968, Harbome 1980, The role of gentisic acid (a p-dihydroxyphenolic add), the compound that shows the highest levels in the neutral and add fractions, could be related to changes in auxin metabolism (Schneider and Wightinan 1974),…”

Section: Discussionmentioning

confidence: 99%

Effects of glyphosate on phenolic metabolism in yellow nutsedge leaves

Cañal

Tumés

Fernández

1987

Physiologia Plantarum

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The action of the herbicide glyphosate [N‐(phosphonomethyl)‐glycine] on phenolic metabolism and phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity was investigated in yellow nutsedge (Cyperus esculentus L.). Glyphosate caused significant increases in the amount of total soluble hydroxyphenolics in the three fractions studied (neutral, acid and residual). Qualitative and quantitative differences in relation to these fractions and the amount of applied glyphosate were observed. Most of the phenolic compounds which increased after glyphosate treatment were benzoic acids (gentisic. p‐OH‐benzoic, salicylic and vanillic). Gentisic acid showed the greatest increase in neutral and acid fractions, being twenty‐ and four‐fold, respectively, of the amount found in the control. PAL activity was not affected by the lowest doses of glyphosate (10−4and 10−3M), but a dramatic decrease in PAL activity was observed after 10−2M treatment. These findings, together with the low levels of cinnamic acids measured in treated yellow nutsedge plants, suggest that PAL activity is only marginally involved in glyphosate action. Since the herbicidal action probably takes place at 5‐enol‐pyruvylshikimate‐3‐P synthase (EC 2.5.1.19), an alternative pathway to PAL in phenolic biosynthesis should be activated yielding benzoic acids.

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“…The expression of major gene resistance to Peronospora farinosa in sugar beet callus cultures Ingram & Robertson (1969, Ingram (1967 and Robertson et al (1968) found that major genes for resistance to Phytophthora infestans appeared to be expressed in potato tissue cultures. Similarly, Warren & Routley (1970) showed that single genes for resistance to P. infestans were at least partially expressed in tomato callus.…”

Section: Sporulation and Pathogenicity Of Peronospora Farinosa Grown mentioning

confidence: 99%

The Growth of Peronospora farinosa f. sp. betae and Sugar Beet Callus Tissues in Dual Culture

Ingram¹,

Joachim²

1971

Journal of General Microbiology

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S U M M A R YCallus cultures established from various sugar beet varieties on complex and chemically defined media were infected with the sugar beet downy mildew fungus Peronospora farinosa under sterile conditions. The host callus and the parasite grew in complete balance in culture, and new infected cultures could be established by transferring explants of infected callus to fresh medium. Peronospora farinosa in callus culture produced normal intercellular hyphae with digitate haustoria and conidiophores with conidia, and remained pathogenic to sugar beet plants for nearly 2 years. The fungus grew for short distances away from infected calluses on the surface of an agar culture medium, but did not grow axenically when connections with these calluses were severed. Resistance to P. farinosa was not expressed normally in sugar beet calluses.

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The Accumulation of Phenolic Acids in Tissue Culture Pathogen Combinations of Solanum tuberosum and Phytophthora infestans

Cited by 29 publications

References 14 publications

Pisatin Production by Tissue Cultures of Pisum sativum L.

Pisatin Production by Tissue Cultures of Pisum sativum L.

Effects of glyphosate on phenolic metabolism in yellow nutsedge leaves

The Growth of Peronospora farinosa f. sp. betae and Sugar Beet Callus Tissues in Dual Culture

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