The Anti-Cancer Effect of Watercress (Rorripa Nasturtium Aquaticum) Extract on Breast Cancer Cells

Fallah, Neda; Kalaee, Soheila Ebrahimi Vosta

doi:10.17795/zjrms-2725

Cited by 9 publications

(11 citation statements)

References 12 publications

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“…In the study conducted by Sefidkon et al [ 7 ], the flavonoid compounds in the dichloromethane fraction obtained from leaves and flowers of Nasturtium officinalis possessed anticancer effects against breast cancer cells (T47D) and colon cancer cells (HT-29). In another study conducted by Fallah and Ebrahimi [ 8 ] the hydroalcoholic extract obtained from Nasturtium aquaticum inhibited the growth of breast cancer cells (MDA-MB-231 cells). Similarly, the treatment of lung carcinoma A549 cells with Nasturtium officinale extract up-regulated the expression of p53, Bax and caspase-3 genes, confirming its anticancer effects against lung cancer [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoliposome-Loaded Phenolics from Nasturtium officinale Improves Health Parameters in a Colorectal Cancer Mouse Model

Taghavinia

Teymouri

Farokhrouz

et al. 2022

Animals

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Nasturtium officinale contains high amounts of phytochemical compounds that work against oxidative damages leading to improved health conditions in animals as well as humans. The study was performed to investigate the health benefits of nonencapsulated and nanoliposome-encapsulated phenolic rich fractions obtained from Nasturtium officinale on mice induced colorectal cancer. The experiment focused on encapsulation efficiency in improving the effectiveness of plant bioactive compounds. Phenolic rich fractions (PRF) were successfully loaded in the nanoliposome structure, a nanometer in size, of spherical shape and with homogeneous dispersion. Induction of colorectal cancer in mice impaired weight gain and feed intake, liver function and structural characteristics of ileum, while the dietary administration of nanoliposome-encapsulated PRF regulated the expression of Caspase 3, Bax, Bcl2, iNOS and SOD genes in the tumor tissue. The addition of nonencapsulated PRF and nanoliposome encapsulated PRF at the concentration of 100 mg TPC/kg BW/day improved the genes expression, although the nanoliposome-encapsulated PRF revealed better health outcomes compared to nonencapsulated PRF. Furthermore, both PRF improved intestinal morphology when the mice were challenged with colorectal cancer. The higher health promoting activity of nanoliposome-encapsulated PRF could be associated with its enhanced intestinal absorption, bioavailability, bioaccessibility and bioactivity. Consequently, the nanoliposome-encapsulated PRF could be considered as a promising anticancer agent against colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Nanoliposome-Loaded Phenolics from Nasturtium officinale Improves Health Parameters in a Colorectal Cancer Mouse Model

Taghavinia

Teymouri

Farokhrouz

et al. 2022

Animals

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“…The toxicity effect of the aqueous extract on the viability of cancer cells and control cells was measured by applying different concentrations of the extract to the rows of plate wells for 24 and 48 hours and then conducting the MTT (methylthiazol tetrazolium) assay (It should be noted that in this study, the idea of determining different times was taken from previous studies on the anti-cancer effects of this plant extract on different cancers). [ 15 16 17 18 ]…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

“…The lack of exposure of cancer and control cells to the extract (i.e., exposure to zero concentration) was considered the negative control. [ 15 16 ] For the MTT assay, 50 μL of the MTT reagent was added to the plates. About 5000 cancer cells were cultured in four rows of a 96-well flat-bottom plate with 50 μL of the medium and kept in an incubator for 24 hours under the same conditions.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

Effect of Nasturtium Extract on Oral Cancer

Nilash

Jahanbani

Jolehar

2023

Advanced Biomedical Research

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Background: Considering the global prevalence of cancers and the complications of common cancer treatments, there is growing interest in using medicinal herbs to complement cancer treatments and reduce treatment's side effects. Therefore, we investigate the effect of the extract of Nasturtium on the viability of oral cancer cells. Materials and Methods: In this experimental study, we prepared aqueous extract from Nasturtium leaves and human oral cancer cells (OCC-24) and normal fibroblast cells (HF2FF cell line) from a cell bank. Then the toxic effect of different concentrations of the extract on cell viability after 24–48 hours of exposure was investigated with the methylthiazol tetrazolium assay. Ultimately, the optical density was measured at 570 nm by an Elisa Reader. Analysis of inhibitory concentration 50 (IC50) was also performed. The data were analyzed by paired Student's t-test and one-way analysis of variance. Results: Data showed that the extract had statistically significant anticancer effects in concentrations above 0.125 mg/ml for 24-hour exposure and in concentrations above 0.5 mg/ml for 48-hour exposure (p-value <0.05). Also, this extract had an adverse effect on the viability of normal cells; however, this effect occurred in high doses of the extract (p-value <0.05). Analysis of IC50 criteria indicates that with increasing time, a higher concentration of the extract is required to inhibit the viability of cancer cells. Conclusion: Because of the results, this aqueous extract can be suggested as a potential therapeutic agent in oral cancer. The best concentration of the extract was found to be 1 mg/ml.

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“…The human esophageal cancer cell line of KYSE-30 and the HF2FF fibroblast cell line, as normal cells (25,29,30), were purchased from the Pasteur Institute of Iran. The KYSE-30 cells were cultured in RPMI-1640/Ham's F12 (Gibco, New York, USA), and the HF2FF cells were seeded in RPMI-1640 (Gibco, New York, USA) medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Bio Sera, Kansas City, MO, USA) (31-34), 100 U/mL penicillin, and 100 mg/mL streptomycin (Gibco, New York, USA).…”

Section: Cell Culturementioning

confidence: 99%

Anti-cancer Effects of Pomegranate Seed Oil on Esophageal Cancer Cell Line (KYSE-30)

2021

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Background: Esophageal cancer is the eighth most common cancer and the sixth most frequent cancer-related death. Despite improvements in treatment approaches, it has remained one of the most challenging cancers for treatment. Thus, it is necessary to introduce novel and efficient methods for treatment. In this regard, the anti-cancer and antioxidant properties of pomegranate have been more concerned. Objectives: The present study aimed to assess the potential cytotoxicity effects of pomegranate on the esophageal cancer cell line. Methods: We cultured esophageal cancer cell line (KYSE-30) and fibroblast cell line (HF2FF) as normal cell line in RPMI-1640/Ham's F12 and RPMI-1640 medium, respectively, with different concentrations of pomegranate seed oil (2, 1, 0.5, 0.25, 0.125, 0.0625, and 0.03125 mg/mL). Then, we evaluated the cytotoxic effects of pomegranate seed oil via morphologic observation and MTT assay after 24, 48, and 72 hours. The wound-healing assay was used for the evaluation of the mobility and migration potential of the treated cells. Results: The results of the MTT assay showed the cytotoxicity and growth inhibitory effects of pomegranate seed oil on esophageal cancer cells. The viability of tumor cells was significantly reduced compared with the untreated control cells and normal cells (P < 0.05), which were dose and time-dependent. The results of the wound-healing assay indicated that pomegranate seed oil could reduce the migration ability of KYSE-30 cells. Conclusions: According to the results, pomegranate seed oil seems to have cytotoxicity effects on esophageal cancer cells. Thus, it might be considered a new, cheap, and safe treatment option for esophageal cancer.

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The Anti-Cancer Effect of Watercress (Rorripa Nasturtium Aquaticum) Extract on Breast Cancer Cells

Cited by 9 publications

References 12 publications

Nanoliposome-Loaded Phenolics from Nasturtium officinale Improves Health Parameters in a Colorectal Cancer Mouse Model

Nanoliposome-Loaded Phenolics from Nasturtium officinale Improves Health Parameters in a Colorectal Cancer Mouse Model

Effect of Nasturtium Extract on Oral Cancer

Anti-cancer Effects of Pomegranate Seed Oil on Esophageal Cancer Cell Line (KYSE-30)

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