The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-κB pathway

Chen, Xin; Miao, Jingshan; Wang, Hao; Zhao, Fang; Hu, Jie; Gao, Peng; Wang, Yue; Zhang, Luyong; Yan, Ming

doi:10.1016/j.jep.2015.05.004

Cited by 40 publications

(19 citation statements)

References 29 publications

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“…Normally, TNF‐α, IL‐1β, and IL‐6 are cytokines that play a significant role in immunity. TNF‐α is the earliest mediator for inflammatory reactions (McCoy, Ruhn, Blesch, & Tansey, ) and a critical inflammation cytokine through activating T cells and upregulating other pro‐inflammatory cytokines (Chen et al, ). IL‐1β plays a vital role in cytokine network and T‐cell activation.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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Immunomodulatory effects of water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP), and concentrated alkali-soluble polysaccharide (CASP) from purple sweet potato were evaluated by using lipopolysaccharide (LPS) treated RAW264.7 macrophage cell models. Results showed WSP, DASP, and CASP (100, 200, and 400 lg/mL) could suppress abnormal phagocytosis, overproduction of TNF-a and IL-6, and abnormal apoptosis caused by LPS. The high doses (200 and 400 lg/mL) of DASP and CASP could distinctly reduce excessive NO production as compared with LPS treatment group. In addition, 400 lg/mL of WSP, DASP, and CASP treatment groups exhibited dramatic decrease in the overexpression of IL-1b stimulated by LPS. Moreover, 200 and 400 lg/mL of WSP, DASP, and CASP markedly improved anti-inflammatory cytokine IL-10 as compared with LPS treatment group. Furthermore, 400 lg/mL of WSP and DASP treatments significantly increased the level of IL-10 in nonstimulated macrophages when compared with normal control group. Practical applicationsPurple sweet potato [Ipomoea batatas (L.) Lam] is one type of highly nutritional vegetable containing many vitamins, minerals, dietary fiber, anthocyanin, and polysaccharides. In addition, it is also one of health-promoting edible plant sources. Recently, polysaccharides have attracted extensive attentions due to their various biological activities. Our study reveals that polysaccharides extracted from purple sweet potato are effective in protecting macrophages damaged by lipopolysaccharide. Therefore, polysaccharides from purple sweet potato have anti-inflammatory efficacy.Thus, this study indicates the potential applications of purple sweet potato polysaccharides in functional food.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

et al. 2018

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“…Regarding this last class of compounds, the derived caffeoylquinic acids appear to have important antiinflammatory actions and inhibit the nuclear transcription factor kappa B (NF-κB) pathway, an important intracellular signaling route responsible for the expression of proinflammatory genes involved in the development and progression of many inflammatory diseases, including asthma [23][24][25][26]. The anti-inflammatory activity of a mixture containing dicaffeoylquinic acids (diCQAs) has been tested in our research group, which have also demonstrated inhibitory effects on p65 NF-κB phosphorylation [27].…”

Section: Introductionmentioning

confidence: 99%

Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia

Facchin

Rosa

Luz

et al. 2020

Mediators of Inflammation

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Objective. The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from Calea pinnatifida leaves in a mouse model of pulmonary neutrophilia. Methods. The CE and derived fractions, hexane, ethyl acetate, and methanol, were obtained from C. pinnatifida leaves. The compounds 3,5- and 4,5-di-O-E-caffeoylquinic acids were isolated from the EtOAc fraction using chromatography and were identified using infrared spectroscopic data and nuclear magnetic resonance (1H and 13C NMR). Leukocytes count, protein concentration of the exudate, myeloperoxidase (MPO) and adenosine deaminase (ADA), and nitrate/nitrite (NOx), tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), and interleukin-17A (IL-17A) levels were determined in the pleural fluid leakage after 4 h of pleurisy induction. We also analyzed the effects of isolated compounds on the phosphorylation of both p65 and p38 in the lung tissue. Results. The CE, its fractions, and isolated compounds inhibited leukocyte activation, protein concentration of the exudate, and MPO, ADA, NOx, TNF-α, IL-1β, and IL-17A levels. 3,5- and 4,5-di-O-E-caffeoylquinic acids also inhibited phosphorylation of both p65 and p38 (P<0.05). Conclusion. This study demonstrated that C. pinnatifida presents important anti-inflammatory properties by inhibiting activated leukocytes and protein concentration of the exudate. These effects were related to the inhibition of proinflammatory mediators. The dicaffeoylquinic acids may be partially responsible for these anti-inflammatory properties through the inhibition of nuclear transcription factor kappa B and mitogen-activated protein kinase pathways.

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“…Excessive inflammatory responses can be harmful, as in diseases such as rheumatoid arthritis, Alzheimer's disease and septic shock syndrome 4) . Lipopolysaccharide (LPS), a component of the cell wall of Gram-negative bacteria, stimulates macrophages to produce pro-inflammatory mediators such as tumor necrosis factor alpha, interleukin-6, and inducible nitric oxide synthase, which trigger a cascade responsible for the inflammatory response 5) . Antioxidants can protect against the damage caused by free radicals that have been implicated in the etiology of large number of major diseases 6) .…”

Section: ⅰ Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages

Kim¹,

Kim²,

Kim³

2016

The Journal of Korean Medicine Ophthalmology and Otolaryngology

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Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin E 2 (PGE 2 ) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability.Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of PGE 2 was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method.Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and PGE 2 production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.

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The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-κB pathway

Cited by 40 publications

References 29 publications

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

Immunomodulatory effects of polysaccharides from purple sweet potato on lipopolysaccharide treated RAW 264.7 macrophages

Systemic Administration of Calea pinnatifida Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia

Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages

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