The antibacterial mechanism of berberine againstActinobacillus pleuropneumoniae

Abstract

This study demonstrated berberine to be a potential natural compound against Actinobacillus pleuropneumoniae. Liquid doubling dilution, transmission electron microscopy (TEM), SDS-PAGE and 4',6-diamidino-2-phenylindole (DAPI) staining were employed to elucidate the antibacterial activity and mechanism of berberine. The minimal inhibitory concentration of berberine was 0.3125 mg/mL, and time-kill curves showed concentration and time dependence. The TEM micrographs displayed damaged cell wall, concentrated cytoplasm, cytoplasmic content leakage and cell death. SDS-PAGE and DAPI assays revealed that berberine can restrain DNA and protein syntheses. Berberine inhibited the synthesis of proteins associated with the growth and cleavage of bacteria and then blocked the division and development of bacteria. The compound ultimately induced cytoplasm pyknosis and bacterial death.

Glycin-rich antimicrobial peptide YD1 from B. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid DNA of E. coli

“…This antibacterial action might result from effects on cellular metabolism. Kang et al (2015) and Li et al (2014) also reported the similar observation of morphological alteration of E. coli cells when treated with berberin and α-terpineol respectively (Kang et al 2015; Li et al 2014). In Fig.…”

Berberine and barberry (Berberis vulgaris): A clinical review

“…Berberine has been shown to have antibacterial activity against a variety of bacteria, including Actinobacillus pleuropneumoniae L. (Kang et al, ), Shigella dysenteriae L. (Kong, Li, Xiao, Zhao, & Zhang, ), Streptococcus agalactiae L. (Peng et al, ), and Helicobacter pylori L. (Zhang et al, ). Berberine has a synergistic effect with some common antibiotics, and this suggests the potential use of berberine in combination with other antibiotics as an efficient therapeutic tool for antibiotic‐resistant bacterial infections.…”

Berberis Vulgaris and Berberine: An Update Review

“…Berberine is widely used as an anti‐infective in traditional medicine (Boberek et al ., ). Berberine has been shown to exhibit antibacterial activity against a variety of bacteria, including Streptococcus agalactiae (MIC = 78 mug/mL) (Peng et al ., ), Actinobacillus pleuropneumoniae (MIC = 0.3125 mg/mL) (Kang et al ., ), different strains of Staphylococccus (MIC = from 16 to 512 micro g/mL) (Wojtyczka et al ., ) and, Shigella dysenteriae (Kong et al , ). Berberine also effectively prevents the formation of S .…”