The antifeedant, insecticidal and insect growth inhibitory activities of triterpenoid saponins fromClematis aethusifoliaTurcz againstPlutella xylostella(L.)

Abstract

BACKGROUND: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a global insect pest of crops, leading to a reduction of agricultural products in productivity and quality. Plant saponins are rich sources for the discovery of candidates to control insect pests. This study focused on discovery of triterpenoid saponins from Clematis aethusifolia Turcz and evaluation of their antifeedant, insecticidal and insect growth inhibitory activities against the 3rd instar larvae of P. xylostella. RESULTS: Seven triterpenoid saponins (1-7) were isolated for the first time from the n-BuOH extract of C. aethusifolia. Monodes

Insecticidal activity of the extract, fractions, and pure steroidal saponins of Trillium govanianumWall. ex D. Don for the control of diamondback moth (Plutella xylostellaL.) and aphid (Aphis craccivoraKoch)

“…The triterpenoid and steroid saponins isolated from plants also showed efficacy against insect pests attacking plants/crop plants and stored products 30 . Triterpenoid saponins (Monodesmosides 1, 2, and 5) isolated from Clematis aethusifolia Turcz showed potent activity (LC 50 = 1463–1786 g L −1 ) against P. xylostella within 72 h 31 . In a similar study, saponin tomentoside A (LC 50 = 1.9 mg mL −1 ), clematoside S (LC 50 = 2.6 mg mL −1 ) isolated from Clematis graveolens Lindl as well as tea saponins were found to be effective against A. craccivora (Rattan et al .)…”

Identification of anti‐TMV active flavonoid glycosides and their mode of action on virus particles from Clematis lasiandra Maxim

“…The air‐dried aerial part of C. lasiandra (5.0 kg) was ground and soaked with 70% EtOH (40 L) at room temperature four times (for 1 week each time). The concentrated EtOH extract (0.72 kg) was suspended in H 2 O and extracted successively with petroleum ether (PE), ethyl acetate (EtOAc) and normal butanol ( n ‐BuOH) according to the previous procedure 22 . The EtOAc extract (43.0 g) was separated into 19 fractions (EFr.A–EFr.S) using CC on silica gel H, eluted with PE/EtOAc (100:1, 20:1, 10:1, 5:1, 3:1 and 1:1) and CH 2 Cl 2 /MeOH (20:1, 10:1 and 1:1) as the gradient elution system.…”

“…The concentrated EtOH extract (0.72 kg) was suspended in H 2 O and extracted successively with petroleum ether (PE), ethyl acetate (EtOAc) and normal butanol (n-BuOH) according to the previous procedure. 22 The EtOAc extract (43.0 g) was separated into 19 fractions (EFr.A-EFr.S) using CC on silica gel H, eluted with PE/EtOAc (100:1, 20:1, 10:1, 5:1, 3:1 and 1:1) and CH 2 Cl 2 /MeOH (20:1, 10:1 and 1:1) as the gradient elution system. EFr.H (3.91 MeOH/H 2 O (100:1:0, 50:1:0, 25:1:0, 15:1:0, 10:1:0.1, 9:1:0.1, 8:2:0.2, 7:3:0.3, 6.5:3.5:0.35 and 1:1:0) as the gradient elution system.…”

“…19,20 As part of our project on the discovery of novel antiviral constituents from terrestrial plants, the aerial part of Clematis lasiandra Maxim, collected from Qin Mountains, Shaanxi Province, China was studied. Our previous studies on chemical constituents from the genus Clematis have demonstrated that triterpenoid saponins have antitumor and insecticidal activities, 21,22 and lignanoids are nontoxic constituents that show potent antibacterial activities. 23,24 Herein, we report the isolation and identification of a new flavonoid glycoside and nine known flavonoids from C. lasiandra.…”

Plant Secondary Metabolites as Defense Tools against Herbivores for Sustainable Crop Protection

“…The following supporting information can be downloaded at: [ 162 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 ].…”