The Antimicrobial Resistance and Prevalence of Enterococcus Species in Saudi Arabia

Alsanie, Walaa F.; Felemban, Ebaa Marwan; Shafie, Alaa; Alhomrani, Majid; Habeeballah, Hamza; Alsharif, Khalaf F.; Farid, Mona A.

doi:10.22207/jpam.13.4.61

Cited by 3 publications

(2 citation statements)

References 26 publications

(29 reference statements)

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“…Similar findings were also reported in Ethiopia of Gondar (6.2%) [24] and Jimma (5.5%) [25]. However, it was lower than the previous report from Saudi Arabia (31.71%) [23], Tanzania (15.9%) [26], India (13.9%) [27], Saudi (14.1%) [28], Poland [29], the USA (45.2%) [30], and Dessie Ethiopia (37.3%) [31]. The current study was also higher than previous studies from different hospitals in Ethiopia such as Jimma (0.59%) [32], Felege Hiwot (0.64%) [33], University of Gondar Teaching Hospital (2.13%) [34], and Kenya (0.22%) [35].…”

Section: Discussionsupporting

confidence: 85%

Asymptomatic Carriage Rate, Multidrug Resistance Level, and Associated Risk Factors of Enterococcus in Clinical Samples among HIV‐Positive Patients Attending at Debre Birhan Comprehensive Specialized Hospital, North Showa, Ethiopia

Tilahun

Gedefie

Sahle

2023

BioMed Research International

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Background. Enterococci are facultative anaerobic, Gram-positive bacteria found in pairs and short chains that exist as normal microflora both human and animal. Enterococci have become a substantial source of nosocomial infections in immunocompromised patients, such as urinary tract infection (UTI), bacteremia, endocarditis, and wound infection. Earlier antibiotic therapy, length of hospital stays, and length of earlier vancomycin treatment, surgical wards, or intensive care units are all risk factors. Additionally, the presence of coinfections such as diabetes and renal failure and the presence of a urinary catheter were aggravated factors to develop infections. Data on the prevalence, antimicrobial susceptibility patterns, and associated factors of enterococcal infection among HIV-positive patients are scarce in Ethiopia. Objective. To determine the asymptomatic carriage rate, multidrug resistance pattern, and risk factors of enterococci in clinical samples among HIV-positive patients attending at Debre Birhan Comprehensive Specialized Hospital, North Showa, Ethiopia. Methods. A hospital-based cross-sectional study was conducted from May to August 2021, at Debre Birhan Comprehensive Specialized Hospital. To obtain sociodemographic data and possible associated factors of enterococcal infections, a pretested structured questionnaire was utilized. During the study period, clinical samples such as urine, blood, swabs, and other bodily fluids from participants sent to the bacteriology section for cultures were included. The study comprised a total of 384 HIV-positive patients. Enterococci were identified and confirmed using bile esculin azide agar (BEAA), Gram stain, catalase response, growth in broth containing 6.5% NaCl, and growth in BHI broth at 45°C. Data were entered and analyzed using SPSS version 25. P values < 0.05 with 95% confidence interval were considered statistically significant. Result. The overall asymptomatic carriage rate of enterococcal infection was 8.85% (34/384). Urinary tract infections were the most common, followed by wounds and blood. The vast majority of the isolate was found in urine, blood, and wound and fecal, 11 (32.4%), 6 (17.6%), and 5 (14.7%), respectively. Overall, 28 (82.35%) bacterial isolates were resistant to three and more than three antimicrobial agents. Duration of hospital associated with >48-hour hospital stays ( AOR = 5.23 , 95% C.I: 3.42-24.6), previous history of catheterization ( AOR = 3.5 , 95% C.I: 5.12-44.31), WHO clinical, stage IV ( AOR = 1.65 , 95% C.I: 1.23-3.61), and CD 4 count < 350 ( AOR = 3.5 , 95% C.I: 5.12-44.31) ( P < 0.05 ). All were associated with higher level of enterococcal infection than their respective groups. Conclusion and Recommendation. Patients with UTIs, sepsis, and wound infection had a greater rate of enterococcal infection than the rest of the patients. Clinical samples in the research area yielded multidrug-resistant enterococci, including VRE. The presence of VRE suggests that multidrug-resistant Gram-positive bacteria have fewer antibiotic treatment options.

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Section: Discussionsupporting

confidence: 85%

Asymptomatic Carriage Rate, Multidrug Resistance Level, and Associated Risk Factors of Enterococcus in Clinical Samples among HIV‐Positive Patients Attending at Debre Birhan Comprehensive Specialized Hospital, North Showa, Ethiopia

Tilahun

Gedefie

Sahle

2023

BioMed Research International

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“…The 16S rRNA gene sequence of the two species were compared with other S. aureus and E coli strains that stored in GenBank database. It was documented that ribosomal genes are significantly applicable for classified bacterial species 30 . When re-constructing phylogenetic tree of bacteria species, sequencing of 16S rRNA has been commonly used.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity And Genotoxicity Effect of Ethanolic Leaves Extract of Rosmarinus Officinalis

Althobaiti¹

2020

J Pure Appl Microbiol

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The recent increasing use of artificial antibiotics has prompted an expansion in resistant strains and high site reactions. Medicinal plants have for quite some time been utilized as traditional medicine to treat pathogenic bacteria. In such manner, consistently numerous scientists are sending a range of plant’s secondary compounds to the customer advertise for the treatment of human illnesses. Accordingly, the distinguishing proof of plant spices with antimicrobial impacts can assist with delivering new medications with a wide range of impacts. The aim of the present research was to examine the ability of ethanolic leaves extracts of Rosmarinus officinalis plant as antibacterial agent against the Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli. The zone of inhibition increased with increase in concentration of the test solution. Higher activity of ethanolic extract was found against S. aureus (2.4 cm) than E. coli (1.8 cm). In addition, the repetitive element PCR (Rep-PCR) significantly showed that several genetic numbers of polymorphic bands were observed in S. aureus and E. coli treated bacteria with leaves extracts and not observed in the control. These results indicate that these extracts have a genotoxicity effect on the two bacterial genomes. The obtained results demonstrate that R. officinalis can be used as a potential source of antibacterial and genotoxicity factors.

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Repurposing fusidic acid as an antimicrobial against enterococci with a low probability of resistance development

Abdelmassih,

Ismail,

Kashef

et al. 2024

Int Microbiol

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Drug repurposing constitutes a strategy to combat antimicrobial resistance, by using agents with known safety, pharmacokinetics, and pharmacodynamics. Previous studies have implemented new fusidic acid (FA) front-loading-dose regimens, allowing higher serum levels than those achievable with ordinary doses. As susceptibility breakpoints are affected by serum level, we evaluated the repurposing of FA as an antimicrobial product against enterococci. FA minimum inhibitory concentrations (MICs) against standard enterococci strains; Enterococcus faecalis ATCC 29212 and Enterococcus faecium ATCC 27270 were 2 and 4 µg/mL, respectively. The MIC against 98 enterococcal clinical isolates was ≤ 8 µg/mL; all would be susceptible if categorized according to recalculated breakpoints (≥ 16 µg/mL), based on the serum level achieved using the front-loading regimen. FA administration in vivo, using the BALB/c mouse infection model, significantly reduced bacterial burden by two to three log10 units in the liver and spleen of mice infected with vancomycin-susceptible and -resistant strains. Exposure of the standard enterococcal strains to increasing, but not fixed, FA concentrations resulted in resistant strains (MIC = 128 µg/mL), with thicker cell walls and slower growth rates. Only one mutation (M651I) was detected in the fusA gene of the resistant strain derived from serial passage of E. faecium ATCC 27270, which was retained in the revertant strain after passage in the FA-free medium. In conclusion, FA can be repurposed as an antimicrobial drug against enterococci with a low probability of mutational resistance development, and can be employed for treatment of infections attributable to vancomycin-resistant enterococci.

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The Antimicrobial Resistance and Prevalence of Enterococcus Species in Saudi Arabia

Cited by 3 publications

References 26 publications

Asymptomatic Carriage Rate, Multidrug Resistance Level, and Associated Risk Factors of Enterococcus in Clinical Samples among HIV‐Positive Patients Attending at Debre Birhan Comprehensive Specialized Hospital, North Showa, Ethiopia

Asymptomatic Carriage Rate, Multidrug Resistance Level, and Associated Risk Factors of Enterococcus in Clinical Samples among HIV‐Positive Patients Attending at Debre Birhan Comprehensive Specialized Hospital, North Showa, Ethiopia

Antibacterial Activity And Genotoxicity Effect of Ethanolic Leaves Extract of Rosmarinus Officinalis

Repurposing fusidic acid as an antimicrobial against enterococci with a low probability of resistance development

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