The Antioxidant Effect of PLGA Nanoparticles Encapsulated with Lutein in Rats Treated with Hypercaloric Diet

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PLGA (Poli-lactic-co-glycolic acid) nanoparticles (NPs) are curently used as drug delivery systems for many types of drugs including antioxidants such as vitamin E. The main aim of our study was to test the antioxidant effects of PLGA-vitamin E on Wistar male rats. Two groups of Wistar rats received a hypercaloric diet for 21 days: the first group received besides the hypercaloric diet a daily dose of PLGA loaded with vitamin E and the control group received only the hypercaloric diet. Spleen cellular lysate has been used to detect biomarkers of oxidative stress such as malondialdehyde, glutathione, advanced human oxidative protein and vitamin E. After 3 weeks of treatment, statistical significant changes have been detected between the two groups.

Section: Resultsmentioning

confidence: 99%

The Antioxidant Effects of PLGA-based Nanoparticles Loaded with Vitamin E in Rats Treated with Hypercaloric Diet

Miricescu¹,

Bălan²,

Radulescu³

et al. 2019

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“…In our previously published studies we have already noticed in Wistar rats that hypercaloric diet leads to OS highlighted by increasing the level of MDA and decreased of GSH level, both liver and spleen. Vitamin E and lutein restores hepatic and splenic redox status by reducing lipid peroxidation and increasing the level of intracellular GSH [35][36][37]. In the last part of our study on Wistar rats, we wanted to see if SO induced by the hypercaloric diet can cause protein oxidation.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity of Entrapped alfa-tocopherol and Lutein in PLGA Nanoparticles in Wistar Rats

Miricescu¹,

Totan²,

Stanescu³

et al. 2019

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The hypercaloric diet leads to obesity which is a risk factor for developing many systemic diseases such as diabetes, cardiovascular diseases and cancer. The purpose of our study was to observe the antioxidants effects of entrapped alfa-tocopherol (vitamin E) and lutein in Poly Lactic-CO-Glycolic acid (PLGA) in Wistar rats that received 3 weeks hypercaloric diet. Hepatic and splenic lysates have been used to detect changes in total antioxidant capacity (TAC) and advanced oxidative protein products (AOPP). The AOPP liver levels were statistically decreased in the case of rats groups that received a daily dose of PLGA-lutein or PLGA-vitamin E versus the control group. The AOPP level was decreased but statistically insignificant in Wistar rats spleen who received a daily dose of PLGA-lutein. At hepatic level, TAC was statistically increased in rats groups that received a daily dose of vitamin E or lutein. An increased statistical level of TAC was observed in the spleen cell lysing in Wistar rats who received the daily dose of PLGA-vitamin E. Entrapment of vitamin E and lutein in PLGA structure has diminished the effects of hypercaloric diet and can be considered a attractive and promising approach to enhance the bioavailability and activity of poorly water soluble compounds.

“…PLGA synthesis and the detection of MDA, vitamin E and GSH have already been described in our previous articles [13,29,30].…”

Section: Biomarkers' Detectionmentioning

confidence: 98%

Hypercaloric Diet and Vitamin E Intake

Radulescu¹,

Miricescu²,

Totan³

et al. 2019

Self Cite

PLGA (poly-lactic-co-glycolic acid) nanoparticles represent an important synthetic biocomponent that has the potential to be a promising carrier of drugs, proteins, nucleic acids, due to its biodegradability and minimal side effects. The aim of our study was to observe the antioxidant effect of vitamin E loaded in PLGA nanoparticles administered over a period of 3 weeks in Wistar rats treated with a hypercaloric diet. Glutathione (GSH) and malondiadehyde (MDA) biomarkers determined from liver lysate were analyzed to evaluate the oxidative stress (OS) induced by the hypercaloric diet. The results of our study revealed a statistically significant increase for GSH and vitamin E in group 2 of Wistar rats receiving hypercaloric diet and a daily dose of vitamin E versus group 1 (p[0.005). The antioxidant effect of vitamin E was also observed by the statistically significant decrease of MDA in group 2 of Wistar rats compared with group 1. The daily dose of vitamin E has improved the liver redox status of group 2 Wistar rats.