The Antiviral Activity of Recombinant Human Tumor Necrosis Factor-α

Effect of Interferon- , Interferon- and Tumour Necrosis Factor on African Swine Fever Virus Replication in Porcine Monocytes and Macrophages

“…Although the molecular basis for the antiviral activity of TNF is unknown, this observation provides a possible explanation for the difference between the results shown here and those published elsewhere (Mestan et aL, 1986;Wong & Goeddel, 1986;Arakawa et al, 1987).…”

“…The extent of such inhibition varies according to the particular virus, the cell type and the type of IFN (~t, fl or y) studied (Domke et al, 1985;DomkeOpitz et al, 1986;Mistchenko & Falcoff, 1987;Horisberger et al, 1983;Belkowski & Sen, 1987;Montefiori & Mitchell, 1987;Kumar et al, 1987;Arakawa et al, 1987;Mestan et al, 1986;Wong & Goeddel, 1986). No investigation has been done with ASF virus.…”

Recognition of Human Tumor Necrosis Factor α (TNF-α) by Therapeutic Antibody Fragment

“…3, b and c) suggests that the transition from the native to the intermediate state is concentration-independent (monomolecular), whereas the transition from the intermediate state to the denatured state is concentration-dependent (non-monomolecular). Based on these qualitative findings, previously reported results on TNF-␣ folding (32), and known TNF-␣ structural features (5,8,9), we assumed that TNF-␣ (T 3 ) unfolding may be described by the following threestate transition model: T 3 (native) 7 I 3 (intermediate) 7 3T D (denatured). Global fitting of the model resulted in its good agreement with the family of TNF-␣ CD denaturation curves measured at different T and different TNF-␣ concentration (Fig.…”

“…Subunits are held together by noncovalent interactions and form a compact bell-shaped trimer characterized by a 3-fold axis of symmetry (5,6). Biologically active TNF-␣ is a homotrimer (7)(8)(9)(10), which may dissociate to monomers in the solution at physiological concentrations (10,11). This regulatory feature has been suggested to be a part of a mechanism essential for maintenance of optimal biological activity of TNF-␣ (10,11).…”

Tumour Necrosis Factor Enhances induction by beta-Interferon of a Ubiquitin Cross-reactive Protein

“…In addition to these varied biological functions, TNFc~ has been shown to have a potent antiviral activity in several different cell lines and against a variety of viruses (Mestan et al, 1986;Wong & Goeddel, 1986;Arakawa et al, 1987;Ruggiero et al, 1989). Recent evidence indicates that TNF-~ acts synergistically with t-interferon (IFN-fl) to establish an antiviral state (Mestan et al, 1988;Reis et al, 1988).…”