The Apoptotic Effect of Gemcitabine-Loaded-Microemulsion (Isopropyl Myristate/Tween 80/Span 20/Water/Ethanol) on A549 Non-Small Cell Lung Cancer Cells

Alkhatib, Mayson H.; Alkhayyal, Norah S.

doi:10.1508/cytologia.81.423

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…In addition, other compounds found among SA extracts in this work (Table 2) including heneicosane, hexadecanoic acid, ethyl ester, lupeol were found to be cytotoxic to HEp-2 (larynx), MCF-7 (breast) and NCI-H292 (lung) cancer cells (Bastos et al, 2017). Isopropyl myristate was cytotoxic to A549 lung cancer cells (Alkhatib and Alkhayyal, 2016). γ-Sitosterol was cytotoxic to A549 (lung) and MCF-7 (breast) (Sundarraj et al, 2012).…”

Section: Effects On Apoptotic Gene Expressionsmentioning

confidence: 59%

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

KARIRAT,

PHANITCHA,

WIBOONCHAT

et al. 2023

Not Bot Horti Agrobo

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Thailand's second-leading cause of death is lung cancer. Thai indigenous herbal plants are sought after as an alternative treatment against lung cancer. This work aimed to examine cytotoxic, antiproliferative and antimigratory capacities of different parts of Streblus asper (SA) from northeastern Thailand on A549 lung cancer cells. Plant leaves, twigs, bark and wood were used for ethanolic extraction by maceration. The highest cytotoxicity of 85.46% was found in twig extract (IC50 57.46 µg/mL) assessed by an MTT assay. The lowest IC50 (18.42 µg/mL) was also found in twig extract using a clonogenic assay indicating its most antiproliferative activity in a long-term therapy. In addition, all SA extracts displayed antimigratory activity against A549 cells in a dose-dependent fashion, especially twig extract. Apoptotic characteristics were noticeable in SA extract treated cells. The maximum DPPH-scavenging activity, FRAP value, total phenolic and flavonoid content were found in twig extract. GC-MS analysis revealed that twig extract contained four prominent components namely ethyl-α-D-glucopyranoside, hexadecanoic acid, ethyl ester, lupeol and γ-sitosterol. Real-time PCR results showed that genes (Bcl-2, Bax, p21, and cytochrome c) linked to apoptosis were significantly affected by all SA extracts. The various SA components' ethanolic extracts exhibited moderate-to-high cytotoxic action towards A549 cells. This work will significantly advance the utilization of the plant as an alternative source of medicine for rural Thais, and it paves the way for future research to determine the active compound(s) and anticipate new drug candidates.

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Section: Effects On Apoptotic Gene Expressionsmentioning

confidence: 59%

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

KARIRAT,

PHANITCHA,

WIBOONCHAT

et al. 2023

Not Bot Horti Agrobo

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“…Therefore, it can be concluded that due to the large amounts of intracellular damage induced by NE-DTX+TQ, the T47D cells respond by activating autophagy with subsequent apoptosis signaling pathways. 31 Taken together, the data suggest that the improved antiproliferative effect of NE-DTX+TQ can be attributed to the triggering of various molecular cascades involved in the cell death mechanisms. 32 Previous studies have reported similar findings regarding the activation of apoptosis simultaneously with autophagy for the nano-delivery system in breast cancer cell lines.…”

Section: Discussionmentioning

confidence: 88%

Growth and invasion inhibition of T47D ductal carcinoma cells by the association of docetaxel with a bioactive agent in neutral nanosuspension

Bawadud

Alkhatib

2023

Bioimpacts

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Introduction: The approach for drug delivery has impressively developed with the emergence of nanosuspension, particularly the targeted nanoemulsions (NEs). It can potentially improve the bioavailability of drugs, enhancing their therapeutic efficiency. This study aims to examine the potential role of NE as a delivery system for the combination of docetaxel (DTX), a microtubule-targeting agent, and thymoquinone (TQ) in the treatment of human ductal carcinoma cells T47D. Methods: NEs were synthesized by ultra-sonication and characterized physically by dynamic light scattering (DLS). A sulforhodamine B assay was performed to evaluate cytotoxicity, and a flow cytometry analysis for cell cycle, apoptosis, autophagy, and cancer stem cell evaluations. A quantitative polymerase chain reaction further assessed the epithelial-mesenchymal transition gene expirations of SNAIL-1, ZEB-1, and TWIST-1. Results: The optimal sizes of blank-NEs and NE-DTX+TQ were found at 117.3 ± 8 nm and 373 ± 6.8 nm, respectively. The synergistic effect of the NE-DTX+TQ formulation significantly inhibited the in vitro proliferation of T47D cells. It caused a significant increase in apoptosis, accompanied by the stimulation of autophagy. Moreover, this formulation arrested T47D cells at the G2/M phase, promoted the reduction of the breast cancer stem cell (BCSC) population, and repressed the expression of TWIST-1 and ZEB-1. Conclusion: Co-delivery of NE-DTX+TQ may probably inhibit the proliferation of T47D via the induction of apoptosis and autophagy pathways and impede the migration by reducing the BCSC population and downregulating TWIST-1 expression to decrease the epithelial-to-mesenchymal transition (EMT) of breast cancer cells. Therefore, the study suggests the NE-DTX+TQ formula as a potential approach to inhibit breast cancer growth and metastasis.

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“…The growth of the MCF-7 cells was greatly impeded when subjected into the LEM-IFO meanwhile SAL-IFO has the best inhibition effect on the growth of the HeLa cells. The great apoptotic effect of LEM-IFO on the MCF-7 cells may stem from the extracellular vesicle formation, seen under the light microscope, indicating that LEM-IFO has got attached into the cell membrane and induced apoptosis integrated with autophagocytosis 22 . In contrast, the light microscopy images revealed that the apoptotic effect of SAL-IFO on the HeLa cells has enlarged the cancer cells and damaged the nucleus without vesicle formation which implies that SAL-IFO has passed the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of ifosfamide into various essential oils -based nanoemulsions ameliorates its apoptotic effect in the cancers cells

Alkhatib

AlMotwaa

Alkreathy

2019

Sci Rep

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The chemotherapeutic drugs, loaded in nanocarriers, have recently attracted the pharmaceutical industries due to their limited adverse side effects. The objective of the current study was to incorporate the ifosfamide (IFO) into two different essential oils-based nanoemulsions, lemon (LEM-IFO) and salvia (SAL-IFO). The antiproliferation activities of the resulted formulas were evaluated in the MCF-7 breast cancer cells and HeLa cervical cancers cells. The cytotoxic effect of the NE formulas was detected by the MTT assay, DAPI stain and light microscopy. The z-average diameters range of LEM-IFO and SAL-IFO, determined by the zetasizer, were 49.15–61.81 nm and 56.64–64.62 nm, respectively. The half maximal inhibitory concentration (IC50) of LEM-IFO and SAL-IFO, applied into the HeLa cells, were 0.165 ± 0.025 and 0.141 ± 0.035 mM, respectively, whereas the IC50 of LEM-IFO and SAL-IFO subjected into the MCF-7 cells were 0.200 ± 0.005 mM and 0.270 ± 0.025 mM, respectively. The IC50 of the free IFO was markedly larger than LEM-IFO and SAL-IFO when applied into MCF-7 cells (9.20 ± 2.01 mM) and HeLa cells (7.69 ± 1.88 mM). Among the tested formulas, LEM-IFO and SAL-IFO have the greatest apoptotic effect on the MCF-7 and HeLa cells, respectively. Solubilizing the IFO in the essential oils-based NE has ameliorated the antitumor efficacy of IFO.

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The Apoptotic Effect of Gemcitabine-Loaded-Microemulsion (Isopropyl Myristate/Tween 80/Span 20/Water/Ethanol) on A549 Non-Small Cell Lung Cancer Cells

Cited by 6 publications

References 25 publications

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells

Growth and invasion inhibition of T47D ductal carcinoma cells by the association of docetaxel with a bioactive agent in neutral nanosuspension

Incorporation of ifosfamide into various essential oils -based nanoemulsions ameliorates its apoptotic effect in the cancers cells

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