The application of biotic elicitor on<i>Artemisia annua</i>L. to increase artemisinin content

Darwati, Ireng; Manohara, Dyah; Rohimatun,; Nurhayati, Hera

doi:10.1088/1755-1315/102/1/012013

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Accordingly, EsxA may be useful as a new protein elicitor that can regulate plant growth. Recent studies proved that protein elicitors can stimulate plant metabolism to modulate plant growth (Darwati et al 2018 ; Shen et al 2019 ). Moreover, interacting proteins that directly or indirectly bind to elicitors have been identified in plants (Mario et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Wang²,

Tang³

et al. 2021

AMB Expr

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An EsxA-encoding gene (esxA) was previously identified in the genome of the plant growth-promoting rhizobacterium Paenibacillus terrae strain NK3-4. The esxA was cloned and expressed in Pichia pastoris, after which the effects of the EsxA protein on rice seedling growth were analyzed to determine whether EsxA contributes to the plant growth-promoting activity of strain NK3-4. The esxA was successfully cloned from the NK3-4 genome and ligated to the eukaryotic expression vector pPICZαA. The resulting pPICZαA-esxA recombinant plasmid was transinfected into yeast cells, and esxA expression in the yeast cells was confirmed. The treatment of seed- buds with the EsxA protein increased the root length by 1.35-times, but decreased the bud length. Additionally, in rice seedlings treated with EsxA, the root and shoot lengths increased by 2.6- and 1.7-times, respectively. These findings imply that EsxA is important for the promotion of rice plant growth by P. terrae strain NK3-4. Furthermore, the construction of the esxA expression vector and the engineered strain may be useful for future investigations of the mechanism underlying the plant growth-promoting effects of EsxA, with implications for the application of EsxA for regulating plant growth.

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Section: Discussionmentioning

confidence: 99%

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Wang²,

Tang³

et al. 2021

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Accordingly, EsxA may be useful as a new protein elicitor that can regulate plant growth. Recent studies proved that protein elicitors can stimulate plant metabolism to modulate plant growth (Darwati et al 2018;Shen et al 2019). Moreover, interacting proteins that directly or indirectly bind to elicitors have been identified in plants (Mario et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression of the EsxA Gene and Analysis of the Growth-Promoting Effects of the Encoded Protein on Rice Seedlings

Wang

Tang³

et al. 2020

Preprint

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An EsxA-encoding gene was previously identified in the genome of the plant growth-promoting rhizobacterium Paenibacillus terrae strain NK3-4. The EsxA gene was cloned and expressed in Pichia pastoris , after which the effects of the EsxA protein on rice seedling growth were analyzed to determine whether EsxA contributes to the plant growth-promoting activity of strain NK3-4. The EsxA gene was successfully cloned from the NK3-4 genome and ligated to the eukaryotic expression vector pPICZαA. The resulting pPICZαA- EsxA recombinant plasmid was inserted into P. pastoris cells, and EsxA gene expression in the yeast cells was confirmed. The treatment of seed- buds with the EsxA protein increased the root length by 1.35-times, but decreased the bud length. Additionally, in rice seedlings treated with EsxA, the root and shoot lengths increased by 2.6- and 1.7-times, respectively. These findings imply that EsxA is important for the promotion of rice plant growth by P. terrae strain NK3-4. Furthermore, the construction of the EsxA gene expression vector and the engineered strain may be useful for future investigations of the mechanism underlying the plant growth-promoting effects of EsxA, with implications for the application of EsxA for regulating plant growth.

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“…Protein elicitors are the most important type of elicitors that activate host plant defense responses (Shen et al 2019 ) to enhance disease resistance (Ruiz et al 2018 ; Mao et al 2010 ; Wang et al 2012 ; Qiu et al 2009 ) and insect resistance (Li et al 2020 ). They also stimulate plant metabolism to regulate plant growth (Darwati et al 2018 ). Recent research on the mechanism underlying elicitor-induced plant stress resistance revealed that pathogenesis-related (PR) proteins may be elicitor receptors that directly or indirectly bind to elicitors, after which they activate a series of downstream defense-related signal transduction pathways, and trigger plant broad-spectrum induced systemic resistance (ISR) to diseases (Liu et al 2018 ; Li et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

Yan

Zheng

et al. 2021

Rice

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Protein elicitors can induce plant systemic resistance to pathogens. In an earlier study, we cloned an EsxA gene from the plant growth-promoting rhizobacterium Paenibacillus terrae NK3-4 and expressed it in Pichia pastoris. In addition to being important for the pathogenicity of animal pathogens, EsxA can also induce an immune response in animals. While, we found the exogenously expressed EsxA has the activity of elicitor, which can trigger hypersensitive response and reactive oxygen species burst in leaves as well as enhanced rice plant growth. The effects of EsxA on seedling blight (Fusarium oxysporum) resistance and gene transcription, including pathogenesis-related (PR) genes in rice were evaluated. The germination rate was 95.0% for seeds treated with EsxA and then inoculated with F. oxysporum, which was 2.8-times higher than that of F. oxysporum-infected control seeds that were not treated with EsxA (Con). The buds and roots of EsxA-treated seedlings were 2.4- and 15.9-times longer than those of Con seedlings. The plants and roots of seedlings dipped in an EsxA solution and then inoculated with F. oxysporum were longer than those of the Con seedlings. Theplant length, number of total roots, and number of white roots were respectively 23.2%, 1.74-times, and 7.42-times greater for the seedlings sprayed with EsxA and then inoculated with F. oxysporum than for the Con seedlings. The EsxA induction efficiency (spray treatment) on seedling blight resistance was 60.9%. The transcriptome analysis revealed 1137 and 239 rice genes with EsxA-induced up-regulated and down-regulated transcription levels, respectively. At 48 h after the EsxA treatment, the transcription of 611 and 160 genes was up-regulated and down-regulated, respectively, compared with the transcription levels for the untreated control at the same time-point. Many disease resistance-related PR genes had up-regulated transcription levels. The qPCR data were consistent with the transcriptome sequencing results. EsxA triggered rice ISR to seedling blight and gene differential transcription, including the up-regulated transcription of rice PR genes. These findings may be relevant for the use of EsxA as a protein elicitor to control plant diseases.

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The application of biotic elicitor onArtemisia annuaL. to increase artemisinin content

Cited by 3 publications

References 15 publications

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Cloning and Expression of the EsxA Gene and Analysis of the Growth-Promoting Effects of the Encoded Protein on Rice Seedlings

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

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The application of biotic elicitor onArtemisia annuaL. to increase artemisinin content

Cited by 3 publications

References 15 publications

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Cloning and expression of the EsxA gene and the growth-promoting effects of the encoded protein on rice seedlings

Cloning and Expression of the EsxA Gene and Analysis of the Growth-Promoting&nbsp;Effects of the Encoded Protein on Rice Seedlings

Protein Elicitor EsxA Induces Resistance to Seedling Blight and PR Genes Differential Transcription in Rice

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Cloning and Expression of the EsxA Gene and Analysis of the Growth-Promoting Effects of the Encoded Protein on Rice Seedlings