The Application of Tissue Culture to Plant Improvement and Propagation in the Ornamental Horticulture Industry

Chu, Irwin Y. E.

doi:10.1007/978-94-009-4444-2_2

Cited by 19 publications

(19 citation statements)

References 12 publications

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“…In order to evaluate the effect of growth hormones on callus induction different concentrations of 2,4-D alone was incorporated in different media – MS, B5 (Gamborg et al 1968 ) and N6 (Chu 1978 ). MS medium with 11.31 μM 2,4-D gave good callus induction with 140 mg fresh weight.…”

Section: Resultsmentioning

confidence: 99%

Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

2013

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BackgroundCereal crops are the major targets for transformation mediated crop improvement and IR36 is an early maturing, high yielding, insect and disease resistant rice variety however, it is abiotic stress sensitive. Hence, development of an efficient and reproducible micropropagation system via somatic embryogenesis and Agrobacterium tumefaciens mediated transformation is prerequisite to develop abiotic stress tolerant IR36. Further, Genetic stability of analysis of plantlets through RAPD and ISSR and Ploidy level through Flow cytometry (FCM) measurement of 2C DNA content is necessary for future application of transformed IR36.ResultsIn this study, Mature seeds inoculated on (Murashige and Skoog) MS medium with 11.31 μM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.3 μM Kinetin (Kn) had highest callus induction frequency (98%). The highest regeneration frequency (80%) was observed in MS + 13.28 μM Benzyladenine (BA) with 8.06 μM α-naphthalene acetic acid (NAA). Randomly Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Flow Cytometry (FCM) analysis showed no significant variation in the 2C DNA (0.81 pg/2C) content and Ploidy level between wild type IR36 and in vitro maintained rice lines. Of the various OD bacterial culture, an optimum OD of 0.4 and inoculation duration of 10 min resulted in efficient Agrobacterium-mediated transformation. β-glucuronidase activity was maximum in callus (99.05%).ConclusionsThese results described here confirm the reliability of this protocol for micropropagation and delivery of desirable gene using A. tumefaciens into indica rice.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-47) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

2013

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“…Tissue culture has been used as a rapid means of vegetative propagation in the ornamental horticulture industry, enabling the production of disease-free propagules that can be readily disseminated (Chu, 1985). Currently, such methods are not available for the propagation of Heliconia spp.…”

mentioning

confidence: 99%

In Vitro Propagation of Heliconia psittacorum by Bud Culture

Nathan¹,

Goh²,

Kumar³

1992

HortSci

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A protocol was developed for in vitro propagation of Heliconia psittacorum L.f. by culture of terminal and axillary buds of rhizomes. Cultures were initiated on modified Murashige and Skoog (MS) medium containing 40 μm BA, 150 ml coconut water/liter, 30 g sucrose/liter, and 2 g Gelrite/liter. Shoot multiplication was achieved on the above medium without coconut water, but supplemented with 10 μm BA. Shoots were rooted on MS basal medium and successfully acclimated to greenhouse conditions. Chemical names used: N-(phenylmethyl)-1 H-purin-6-amine (BA).

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“…Micro-propagation has numerous advantages and applications in commercial nursery operations (Chu, 1986;McCown, 1986;Pierik, 1987;Stimart, 1986). New plantlet within a short period after utilizing a small amount of initial plant tissue, viruses and diseases free plantlet, season independent supply of seedlings throughout the year after utilizing ten times lower space than the conventional technique supplying new subspecies and variety and looking to cultivate challenging plants, such as specific breeds of roses to find more success with the tissue culture process than traditional soil can be ensured by exploiting micropropagation/tissue culture technique (Plant Cell Technology, 2022) in this era of climate change, especially in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

Study on in vitro micropropagation of Rosa sp.

Afrin¹,

Rahman²,

Khalekuzzaman³

et al. 2022

Bangladesh J. Agri.

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Tissue culture has long been recognized as an ideal experimental paradigm for studying the mechanisms governing plant cell growth, division, and physiological and biochemical processes. The present investigation was undertaken to establish a standard method for callus induction, shoot and root regeneration of rose at Genetic Engineering and Biotechnology Laboratory, University of Rajshahi, Bangladesh in 2010. Shoot tip and nodal segments were used as experimental materials excised from field grown plants for callus induction. The direct shoot was observed from both shoot tip and nodal segments for shoot regeneration. In vitro regenerated shoot cuttings were used for root regeneration. Proper manipulation of auxin (2, 4 -D and NAA) was used to induce callus from different explants. Different growth regulators (BAP, KIN and GA3) were casted-off in combination for shoot regeneration and proliferation. Again, different concentrations of auxins (IBA, NAA, and IAA) were applied for root initiation. Among the hormonal supplements used, 2, 4 -D was found best in all respect of callusing response for all types of explants. The highest percentage (90%) of callus induction was observed in media having MS (Murashige and Skoog) + 4.0 mg/L 2, 4 -D. In case of shoot regeneration, 100% of cultured explants regenerated shoot in media with MS + 2.0 mg/L BAP + 0.5 mg/L KIN. While, 2.0 mg/L BAP + 0.5 mg/L KIN + 0.1 mg/L GA3 induced 100% shoot proliferation. Moreover, for root induction, ½ MS + 1.0 mg/L IBA + 1.0 mg/L NAA proved to be the best (80%) from in vitro regenerated shoot cuttings, and the highest mean number of roots was 5.0. Rooted shoots were acclimatized and successfully established in a natural condition where 60% of the transplanted plants survived. Bangladesh J. Agri. 2022, 47(1): 66-74

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The Application of Tissue Culture to Plant Improvement and Propagation in the Ornamental Horticulture Industry

Cited by 19 publications

References 12 publications

Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

Rapid regeneration and ploidy stability of ‘cv IR36’ indica rice (Oryza Sativa. L) confers efficient protocol for in vitro callus organogenesis and Agrobacterium tumefaciens mediated transformation

In Vitro Propagation of Heliconia psittacorum by Bud Culture

Study on in vitro micropropagation of Rosa sp.

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