The application of visible wavelength reflectance hyperspectral imaging for the detection and identification of blood stains

Li, Bo; Beveridge, Peter; O’Hare, W. T.; Islam, Meez

doi:10.1016/j.scijus.2014.05.003

Cited by 41 publications

(47 citation statements)

References 15 publications

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“…Previous work has also investigated changes to the Soret band in the UV-Visible spectrum of haemoglobin [18], although this was purely focused on changes to the wavelength of the Soret band peak over time for age determination only, and did not allow for identification or confirmation of blood. The approach carried out by our research group indicated a higher sensitivity and specificity for the detection and identification of blood stains over previously proposed methods [3]. Recent research has since successfully demonstrated the application of this technique for the detection, identification, and visualisation of ridge detail in blood stained fingerprints deposited on white ceramic tiles [19] and a range of other substrates commonly encountered at scenes of crime [20].…”

Section: Introductionmentioning

confidence: 79%

“…The HSI system used in this study was the same setup detailed in [3] and [19,20], consisting of a liquid crystal tuneable filter (LCTF) coupled to a 2.3 megapixel Point Grey camera and a light source for scene illumination. The light source was comprised of two 40W LEDs; one violet giving an output at 410nm and one white giving an output between 450 and 700 nm.…”

Section: The Hyperspectral Imaging (Hsi) Systemmentioning

confidence: 99%

“…The hyperspectral reflectance measurements were made following the method detailed in [3] and [19,20]. A reference image (R 0 ) was obtained using a blank ceramic tile.…”

Section: Hyperspectral Reflectance Image Acquisition and Pre-processingmentioning

confidence: 99%

“…Analysis of the blood spatter pattern can help with reconstructing events and DNA profiling can be carried out, which may assist to establish the identity of the suspect. The current forensic workflow for the examination of suspected blood stains starts with visual examination, including the use of alternate light sources, to confirm the identity of the substance before further analysis [3]. This is followed by analysis using chemical presumptive tests to provide an indication as to the presence of blood [4].…”

Section: Introductionmentioning

confidence: 99%

“…A similar approach was presented by another research group for the identification of blood stains [15,16], detailing a presumptive method for the rapid non-destructive detection of blood stains. Most recently our research group a new blood stain identification and age determination approach was proposed based on the Soret γ band absorption in haemoglobin [3,17]. Previous work has also investigated changes to the Soret band in the UV-Visible spectrum of haemoglobin [18], although this was purely focused on changes to the wavelength of the Soret band peak over time for age determination only, and did not allow for identification or confirmation of blood.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

Cadd

Beveridge

et al. 2016

Science & Justice

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Bloodstains are often encountered at scenes of violent crime and have significant forensic value for criminal investigations. Blood is one of the most commonly encountered types of biological evidence and is the most commonly observed fingerprint contaminant. Presumptive tests are used to test blood stain and blood stained fingerprints are targeted with chemical enhancement methods, such as acid stains, including Acid Black 1, Acid Violet 17 or Acid Yellow 7. Although these techniques successfully visualise ridge detail, they are destructive, do not confirm the presence of blood and can have a negative impact on DNA sampling. A novel application of visible wavelength hyperspectral imaging (HSI) is used for the noncontact, non-destructive detection and identification of blood stained fingerprints on white tiles both before and after wet chemical enhancement using Acid Black 1. The identification was obtained in a non-contact and non-destructive manner, based on the unique visible absorption spectrum of haemoglobin between 400 and 680 nm. Results from the exploration of the selectivity of the setup to detect blood against ten other non-blood protein contaminants are also presented. A direct comparison of the effectiveness of HSI with chemical enhancement using Acid Black 1 on white tiles is also presented.

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Section: Introductionmentioning

confidence: 79%

Section: The Hyperspectral Imaging (Hsi) Systemmentioning

confidence: 99%

“…The hyperspectral reflectance measurements were made following the method detailed in [3] and [19,20]. A reference image (R 0 ) was obtained using a blank ceramic tile.…”

Section: Hyperspectral Reflectance Image Acquisition and Pre-processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

Cadd

Beveridge

et al. 2016

Science & Justice

Self Cite

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Proteomics goes forensic: Detection and mapping of blood signatures in fingermarks

et al. 2016

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A bottom up in situ proteomic method has been developed enabling the mapping of multiple blood signatures on the intact ridges of blood fingermarks by Matrix Assisted Laser Desorption Mass Spectrometry Imaging (MALDI-MSI). This method, at a proof of concept stage, builds upon recently published work demonstrating the opportunity to profile and identify multiple blood signatures in bloodstains via a bottom up proteomic approach. The present protocol addresses the limitation of the previously developed profiling method with respect to destructivity; destructivity should be avoided for evidence such as blood fingermarks, where the ridge detail must be preserved in order to provide the associative link between the biometric information and the events of bloodshed. Using a blood mark reference model, trypsin concentration and spraying conditions have been optimised within the technical constraints of the depositor eventually employed; the application of MALDI-MSI and Ion Mobility MS have enabled the detection, confirmation and visualisation of blood signatures directly onto the ridge pattern. These results are to be considered a first insight into a method eventually informing investigations (and judicial debates) of violent crimes in which the reliable and non-destructive detection and mapping of blood in fingermarks is paramount to reconstruct the events of bloodshed.

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Sample Treatment for Tissue Proteomics in Cancer, Toxicology, and Forensics

Cole

Clench

Francese

2019

Advances in Experimental Medicine and Biology

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Since the birth of proteomics science in the '90, the number of applications and of sample preparation methods has grown exponentially, making a huge contribution to the knowledge in lifescience disciplines. Continuous improvements in the sample treatment strategies unlocks and reveals the fine details of disease mechanisms, drug potency and toxicity as well as enabling new disciplines to be investigated such as forensic science.

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The application of visible wavelength reflectance hyperspectral imaging for the detection and identification of blood stains

Cited by 41 publications

References 15 publications

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

Proteomics goes forensic: Detection and mapping of blood signatures in fingermarks

Sample Treatment for Tissue Proteomics in Cancer, Toxicology, and Forensics

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