The aqueous extract of <em>Brucea javanica</em> suppresses cell growth and alleviates tumorigenesis of human lung cancer cells by targeting mutated epidermal growth factor receptor

Abstract

As a practical and safe herbal medicine, the seeds of Brucea javanica (L.) Merr., were used to cure patients suffering from infectious diseases such as malaria. Recent advances revealed that the herb could also be a useful cancer therapy agent. The study demonstrated that aqueous B. javanica (BJ) extract attenuated the growth of human non-small-lung cancer cells bearing mutant L858R/T790M epidermal growth factor receptor (EGFR). The reduced cell viability in H1975 cells was attributed to apoptosis. Transfection of EGFR small hairpin RNA reverted the sensitivities. When nude mice were fed BJ extract, the growth of xenograft tumors, as established by H1975 cells, was suppressed. Additional histological examination and fluorescence analysis of the resected tissues proved that the induced apoptosis mitigated tumor growth. The work proved that the BJ extract exerted its effectiveness by targeting lung cancer cells carrying mutated EGFR while alleviating tumorigenesis. Aqueous BJ extract is a good candidate to overcome drug resistance in patients undergoing target therapy.

Effects of Storage Time and Temperature on Antioxidants in Juice from Momordica charantia L. and Momordica charantia L. var. abbreviata Ser.

“…MS detection was arranged as a full scan range from 100 to 1200 amu and operated at an interface voltage of 4.5 kV with a desolvation temperature of 150 °C. In addition, the flow rates of nebulizing and drying gas with nitrogen were 1.5 and 20 L/min, respectively [ 26 ].…”

“…The mobile phase flow rate was 0.4 mL/min, and the column temperature was maintained at 40 • C. UFLC-mass spectrometry (MS) used positive and negative electrospray ionization sources. MS detection was arranged as a full scan range from 100 to 1200 amu and operated at an interface voltage of 4.5 kV with a desolvation temperature of 150 • C. In addition, the flow rates of nebulizing and drying gas with nitrogen were 1.5 and 20 L/min, respectively [26].…”

The apoptotic effects of <em>Brucea javanica</em> fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-&kappa;B translocation

“…The B. javanica fruits were air dried and crushed, and the plant material (300 g) was extracted by the method described by Kim et al with some modifications. 21 The crushed fruits were defatted with 1 L hexane (merckmillipore) by soaking for 3 days, and then the mixture was exhausted and the hexane extract was clarified with filter paper. The resulted hexane extract was concentrated via rotary evaporator and then dry residues of crushed fruits from hexane extract were soaked in 1 L absolute ethanol (99.5% purity) at room temperature for 3 days, and the mixture was filtered.…”

The Chemopreventive Activity of Indonesia Medicinal Plants Targeting on Hallmarks of Cancer

“…28 The aqueous extract of B. javanica attenuates EGFR activity in liver cancer and human non-small-lung cancer cells; resulting in the inhibition of cancer proliferation. 29,30 Ethanolic extract of C. sappan inhibits HER2 expression in breast cancer cells. 31 Interestingly, curcumin shows an impressive activity against various RTKs, including EGFR and HER-2, in colon cancer cells.…”