The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots

Kong, Que; Ma, Wei; Yang, Haibing; Ma, Guojie; Mantyla, Jenny J.; Benning, Christoph

doi:10.1093/jxb/erx275

Cited by 45 publications

(48 citation statements)

References 59 publications

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“…In this study we also observed the badc1 badc2 double mutant exhibits reduced primary root growth, a phenotype that resembled that of the wri1-1 mutant (Kong et al, 2017), which in combination of our finding that WRI1 controls BADC transcription led us to ask whether the short-root phenotype characteristic of both wri1-1 and badc1badc2 mutants result from a deficit of BADC. If correct, overexpression of BADC in wri1-1 would mitigate the wri1-1 short-root phenotype.…”

Section: Discussionmentioning

confidence: 51%

“…1D and 1E). Because the wri1 mutant ( wri1-1 ) was recently shown to have short-root phenotype during seedling establishment (Kong et al, 2017), we compared the growth of badc1 badc2 and wri1-1 under the same conditions and show that their phenotypes are visually indistinguishable (SFig. 2).…”

Section: Resultsmentioning

confidence: 99%

“…In wri1-1 the levels of conjugated auxin, i.e., indole-3-acetic acid (IAA)-Asp, were reported to be significantly elevated compared to WT (Kong et al, 2017). The similarity between the root phenotypes of badc1 badc2 and wri1-1 during seedling establishment prompted us to measure the levels of several growth hormones.…”

Section: Resultsmentioning

confidence: 99%

“…GFP-WRI1 then was inserted into pet28b between XhoI and NcoI by in-fusion cloning. Full length of WRI1 in the resulted GFP-WRI1 /pet28b was replaced with DNA sequence corresponding to WRI1 DNA binding domain (WRI1 58-240 ) between XhoI and BsrGI (Kong et al, 2017). The primer pairs used in building this construct is listed in Table S4.…”

Section: Methodsmentioning

confidence: 99%

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BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins, inhibitors of ACCase, are regulated by WRINKLED1

Liu¹,

Zhai²,

Kuczynski³

et al. 2019

Preprint

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Tel: 631 344 3414 59 60 One sentence summary: 61 WRI1 regulates genes encoding both fatty acid synthesis and inhibitors of FA synthesis (BADCs), 62 creating a lipid homeostatic mechanism in which the transcription of FA synthetic capacity is coordinated 63with the capacity to biochemically downregulate FA synthesis. 64 Running title: WRI1 regulates BADC proteins 65Abstract 80 WRINKLED1 (WRI1) is a transcriptional activator that binds to AW boxes in the promoters of many genes 81 from central metabolism and FA synthesis, resulting in their transcription. BIOTIN ATTACHMENT 82 DOMAIN-CONTAINING (BADC) proteins are homologs of BIOTIN CARBOXYL CARRIER PROTEIN 83 (BCCP) that lack a biotin-attachment domain and are therefore inactive. In the presence of excess FA, 84 BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of 85 ACETYL-COA CARBOXYLASE (ACCase), and consequently FA synthesis. Purified WRI1 bound with 86 high affinity (Kds in the low nanomolar range) to canonical AW-boxes from the promoters of all three 87 BADC genes. Consistent with this observation, the expression of BADC1, BADC2 and BADC3 genes and 88 BADC1 protein levels were reduced in wri1-1 relative to wild type (WT), and BADCs gene expression and 89 BADC1 protein levels also were elevated upon WRI1 overexpression. The double mutant badc1badc2 90 phenocopied wri1-1 with respect to both reduction in root length, and elevation of indole-3-acetic acid-Asp 91 (IAA-Asp) levels relative to WT. Overexpression of BADC1 in wri1-1 decreased its IAA-Asp and partially 92 rescued its short-root phenotype demonstrating a role for BADCs in seedling establishment. That WRI1 93 positively regulates genes encoding both FA synthesis and BADCs i.e., conditional inhibitors of FA 94 synthesis, represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the 95 transcription their FA synthetic capacity with their capacity to biochemically downregulate it. 96 97

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Section: Discussionmentioning

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins, inhibitors of ACCase, are regulated by WRINKLED1

Liu¹,

Zhai²,

Kuczynski³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…WRI1 encodes for a APETALA2/ethylene responsive element binding protein, which is involved in global regulation of key enzymes of carbon metabolism and fatty acid biosynthesis (Cernac and Benning, 2004;Ruuska et al, 2002). Further studies showed that AtWRI1 also plays a role in processes such as lipid assembly, storage, flowering time, seed development, embryo maturation, plant hormone regulation, and photosynthesis (Cernac et al, 2006;Kong et al, 2017;Li et al, 2015;Maeo et al, 2009;Wu et al, 2014). A number of other communications have also revealed orthologs of AtWRI1 being involved in plant oil biosynthesis (Adhikari et al, 2016;Bhattacharya et al, 2016;Chen et al, 2017;Ivarson et al, 2016;Kang et al, 2017;Kim et al, 2016;Liu et al, 2010;Ma et al, 2013;Pouvreau et al, 2011;Shen et al, 2010;Vanhercke et al, 2013;Wu et al, 2014;Yeap et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

Vogel

Noyer

Park

et al. 2019

Plant Biotechnology Journal

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Summary Soybean ( Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed‐specific expression cassette carrying the Arabidopsis transcription factor WRINKLED 1 (At WRI 1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the At WRI 1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl‐ ACP thioesterase (GmFatA) resulted in oil with total saturates up to 30%. The creation of a triple stack in soybean, wherein the At WRI 1 and GmFatA alleles were combined with a FAD 2‐1 silencing allele led to the synthesis of an oil with 28% saturates and approximately 60% oleate. Constructs were then assembled that carry a dual FAD 2‐1 silencing element/GmFatA expression cassette, alone or combined with an At WRI 1 cassette. These plasmids are designated pPTN 1289 and pPTN 1301, respectively. Transgenic events carrying the T‐ DNA of pPTN 1289 displayed an oil with stearate levels between 18% and 25%, and oleate in the upper 60%, with reduced palmitate (<5%). While soybean events harboring transgenic alleles of pPTN 1301 had similar levels of stearic and oleate levels as that of the pPTRN 1289 events, but with levels of palmitate closer to wild type. The modified fatty acid composition results in an oil with higher oxidative stability, and functionality attributes for end use in baking applications.

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Genome-wide analysis reveals the evolution and structural features of WRINKLED1 in plants

Tang

Huan

et al. 2018

Mol Genet Genomics

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The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots

Abstract: WRINKLED1 (WRI1) has targets other than fatty acid biosynthesis genes that mediate the growth of roots.

Cited by 45 publications

References 59 publications

BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins, inhibitors of ACCase, are regulated by WRINKLED1

BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins, inhibitors of ACCase, are regulated by WRINKLED1

Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

Genome-wide analysis reveals the evolution and structural features of WRINKLED1 in plants

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