The association of lipid abnormalities with tissue pathology in human osteoarthritic articular cartilage

Lippiello, Louis; Walsh, Thomas; Fienhold, Margery A.

doi:10.1016/0026-0495(91)90046-y

Cited by 136 publications

(122 citation statements)

References 29 publications

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“…In the case of the cartilage, only few authors have studied the lipid metabolism of chondrocyte cells. A relation between cartilage lesion and alterations in the lipid metabolism has been found by techniques such as electron microscopy (39,40). Finally, FA, DAG and other lipids have been detected in high concentrations in plasma of OA patients by LC-chromatography (41) supporting our results.…”

Section: Pca Revealed the Presence Of Droplets Rich In Lipids In Oa Csupporting

confidence: 84%

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

et al. 2012

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ABSTRACT. Osteoarthritis (OA) is a pathology that ultimately causes joint destruction.The cartilage is one of the principal affected tissues. Alterations in the lipid mediators and an imbalance in the metabolism of cells that form the cartilage (chondrocytes), have been described as contributors to the OA development. In this study, we have studied the distribution of lipids and chemical elements in healthy and OA human cartilage. Time of flight secondary ion mass spectrometry (TOF-SIMS) allows us to study the spatial distribution of molecules at a high resolution on a tissue section. TOF-SIMS revealed a specific peak profile that distinguishes healthy from OA cartilages. The spatial distribution of cholesterol-related peaks exhibited a remarkable difference between healthy and OA cartilages. A distinctive co-localization of cholesterol and other lipids in the superficial area of the cartilage was found. A higher intensity of oleic acid and other fatty acids in the OA cartilages exhibited a similar localization. On the other hand, CNwas observed with a higher intensity in the healthy samples. Finally, we observed an accumulation of calcium and phosphate ions exclusively in areas surrounding the chondrocyte in OA tissues. To our knowledge, this is the first time that TOF-SIMS revealed combined changes in the molecular distribution in the OA human cartilage.

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Section: Pca Revealed the Presence Of Droplets Rich In Lipids In Oa Csupporting

confidence: 84%

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

et al. 2012

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“…Unsaturated fatty acids are liquid at room temperature, in their spectra the 2840cm -1 peak is still present but the peak at 2870cm -1 is less pronounced and incorporated in a much broader peak between arround 2860-2930cm -1 [31,32]. The spectrum of the chondrocyte lipid droplets matches that of unsaturated fatty acids, this is in good aggrement with the data on the overall lipid content of cartilage reported in the literature with the most abundant fatty acids being oleic, palmitic and linoleic (listed in order of decreasing abundance) [33]. …”

Section: C) Srs A) B) Carssupporting

confidence: 81%

Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

et al. 2013

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Stimulated Raman Scattering has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interteritorial matrix. Changes in the cells and pericellular matrix were investigated as a function of depth into the cartilage. Lipid was detected in the pericellular matrix of superficial zone chondrocytes. The spectral profile of lipid droplets within the chondrocytes indicated that they contained predominantly unsaturated lipids. The mineral content has been imaged by using the PO4 3-vibration at 959cm -1 and the CO3 2-vibration at 1070cm -1 . Both changes in cells and mineralization are known to be important factors in the progression of osteoarthritis. SRS enables these to be visualised in fresh unstained tissue and consequently should benefit osteoarthiritis research

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“…Lippiello et al (42) observed that the levels of fatty acids were markedly enhanced in OA cartilage in association with an increasing degree of OA lesions. Certain rare forms of osteochondrodysplasia associated with precocious degenerative joint disease are characterized by the accumulation of lipids in chondrocytes (43,44).…”

Section: Discussionmentioning

confidence: 99%

The presence of molecular markers of in vivo lipid peroxidation in osteoarthritic cartilage: A pathogenic role in osteoarthritis

Shah¹,

Raska²,

Tiku³

2005

Arthritis & Rheumatism

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Objective. To investigate the role of oxidative functions in human osteoarthritic (OA) chondrocytes and to investigate the presence of in vivo molecular markers of lipoxidation in OA cartilage.Methods. An in vitro model of cartilage collagen degradation was used. Lipid peroxidation activity and overall oxidative function in OA chondrocytes were monitored by cis-parinaric acid and dichlorofluorescein assays, respectively. In vivo molecular markers of lipoxidation in normal and OA cartilage were studied using immunohistochemistry to detect the presence of malondialdehyde and hydroxynonenal adducts.Results. Human OA chondrocytes showed a robust amount of 3 H-proline-labeled collagen degradation upon stimulation with lipopolysaccharide and calcium ionophore A21387, as compared with that in untreated OA chondrocytes. Primary OA chondrocytes showed both spontaneous and inducible levels of lipid peroxidation activity. However, lipid peroxidation activity was already maximally elevated in more than 50% of the OA chondrocyte samples. Overall, spontaneous and inducible oxidative activities were observed in all OA samples. Immunohistochemical analysis of control OA tissue sections that were not treated with monoclonal antibody showed little immunoreactivity. OA cartilage sections treated with monoclonal antibodies showed specific immunoreactivity on the cartilage surface, at sites of OA lesions, at the pericellular matrix, and at intra-and intercellular matrices. Normal cartilage sections showed faint surface reactivity.Conclusion. Our observations suggest that human OA chondrocytes demonstrate spontaneous and inducible cell-associated lipoxidative and nonlipoxidative activity. Lipoxidative activity appears to be enhanced in OA chondrocytes. The presence of molecular markers of in vivo lipid peroxidation was demonstrated in OA cartilage, suggesting its role in the pathogenesis of the disease.Osteoarthritis (OA) is the most common form of joint disease that affects humans. The incidence of OA increases during every decade of life, and by the age of 65 years, almost one-third of the population has OA of the knee joints. The economic burden attributed to the joint pain and disability of OA amounts to billions of dollars each year (1). As the population demographic in the US changes to a predominantly older generation, the increasing prevalence of OA will be a major public health problem.There is currently no treatment available that will prevent or cure OA. Pharmacologic and nonpharmacologic agents used for OA provide only symptomatic relief of pain. The lack of specific therapy for this disease is perhaps due to our limited understanding of its pathogenesis. Understanding the molecular mechanisms involved in the development of OA will help us to develop ways to prevent or reverse the degenerative process of the disease.Current concepts of the pathogenic mechanisms of OA suggest that there is a shift in the homeostatic balance between the destruction and synthesis of bone and cartilage, with a net progressive destruction of t...

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The association of lipid abnormalities with tissue pathology in human osteoarthritic articular cartilage

Cited by 136 publications

References 29 publications

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

Time-of-Flight Secondary Ion Mass Spectrometry-Based Molecular Distribution Distinguishing Healthy and Osteoarthritic Human Cartilage

Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

The presence of molecular markers of in vivo lipid peroxidation in osteoarthritic cartilage: A pathogenic role in osteoarthritis

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