The AT thermoluminescence band from Chlamydomonas reinhardtii and the effects of mutagenesis of histidine residues on the donor side of the Photosystem II D1 polypeptide

Kramer, David; Roffey, Robin A.; Sayre, Richard T.

doi:10.1016/0005-2728(94)90214-3

Cited by 45 publications

(29 citation statements)

References 20 publications

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“…Site directed mutagenesis of Chlamydomonas reinhardtii showed that the replacement of His195 in the vicinity of TyrZ did not influence the peak position of the AT band, while the conversion of His190 residue to phenylalanine completely abolished this band (Kramer et al 1994). This is in good agreement with the earlier observation that the tyrosine modifier NBD (7-chloro-4-nitrobenz-2-oxa-1,3-diazole) had no influence on the AT band (Allakhverdiev et al 1992).…”

Section: Introductionsupporting

confidence: 81%

“…The insensitivity of the AT band to Cu(II) poisoning indicates that in TRISwashed BBY particles, Cu(II) does not interfere with either QA− or HisC required to generate the AT band (Ono and Inoue 1991;Allakhverdiev et al 1992). The recent finding that at higher concentrations of Cu(II) or NBD, Tyrz is completely inhibited suggests that HisCQA− recombination does not require active Tyrz and verifies the earlier assumption that the generation of the A and AT bands might have alternative recombination pathways (Kramer et al 1994). This is in accordance with the earlier observation that in the presence of the tyrosine modifier NBD the AT band was absolutely unaffected (Allakhverdiev et al 1992).…”

Section: Resultssupporting

confidence: 56%

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Horváth¹,

Arellano

Droppa³

et al. 1998

Photosynthesis Research

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The thermoluminescence characteristics of functionally intact thylakoids and TRIS-washed BBY particles werestudied under Cu(II) poisoned conditions. In thylakoids, both the A and B thermoluminescence bands correspondingto S3QA− and S2S3QB− charge recombinations, respectively showed specific responses to Cu(II) treatment. The amplitude of the B band was gradually decreased, which corresponds to the Cu(II) induced inactivation ofTyrZ. The simultaneous stepwise shift in the peak position of the B band indicated, however, that S3QB− chargere combination is more resistant to Cu(II) poisoned conditions. The shifted peak position of the A band toward thehigher temperature in Cu(II) treated thylakoids also showed a change in the redox span between the recombination partners generating the A band of the glow curve. The AT band due to the HisCQA− recombination in TRISwashedBBY particles was insensitive to Cu(II) addition indicating that Cu(II) did not affect either HisC or QA−. The unaffected intensity of the A and AT bands when Cu(II) inhibits TyrZ function favours the assumption of an alternative pathway in which functional TyrZ is not required. In addition, Cu-induced changes of the TL bands were compared to those produced by the Tyr and His modifiers NBD and DEPC, respectively.We obtained very similar results regarding TL bands by either adding NBD or Cu-poisoning in functional thylakoids. Regarding DEPC, the A and AT bands were abolished by increasing concentrations of the His modifier. This effect was associated with the decrease of the B band and its replacement by the Q band at around 0 ºC. Comparing our data obtained by Cu, NBD and DEPC treatments, we have found a strong interrelation between HisC and S3 state. We assume that in some inhibitory conditions in the S3 state His is oxidized in place of Mn and this alternative pathway does not require functional TyrZ.Abbreviations: DCMU-3-(3,4-dichlorophenyl)-1,1-dimethylurea; DEPC-diethyl pirocarbonate; NBD-7-chloro-4-nitrobenz-2-oxa-1,3-diazole; PS II -Photosytem II; QA and QB -the primary and secondary quinine acceptors of PS II; TL -thermoluminescence; TyrZ -tyrosine species acting as a donor to P680.

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Section: Introductionsupporting

confidence: 81%

Section: Resultssupporting

confidence: 56%

Untitled

Horváth¹,

Arellano

Droppa³

et al. 1998

Photosynthesis Research

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“…The QM/MM models predict that the proteinaceous ligation of the OEC involves several amino-acid residues already thought to be ligands to the metal cluster on the basis of XRD (Biesiadka et al 2004;Ferreira et al 2004;Loll et al 2005), site-directed mutagenesis and spectroscopic studies (Boerner et al 1992;Chu et al 2004;Chu et al 1994;Chu et al 1995;Clausen et al 2004;Debus 2001;Debus et al 2005;Diner 2001;Kramer et al 1994;Roffey et al 1994;Strickler et al 2006;Strickler et al 2005). The proposed QM/MM coordination is similar to the proposal by Ferreira et al (Ferreira et al 2004), except that D1-Glu333 in the QM/MM models bridges Mn(2) and Mn(3) in a η 2 mode, instead of monodentally binding Mn(4).…”

Section: Protein Ligandsmentioning

confidence: 99%

Computational insights into the O2-evolving complex of photosystem II

et al. 2008

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Mechanistic investigations of the water-splitting reaction of the oxygen-evolving complex (OEC) of photosystem II (PSII) are fundamentally informed by structural studies. Many physical techniques have provided important insights into the OEC structure and function, including X-ray diffraction (XRD) and extended X-ray absorption fine structure (EXAFS) spectroscopy as well as mass spectrometry (MS), electron paramagnetic resonance (EPR) spectroscopy and Fourier transform infrared spectroscopy applied in conjunction with mutagenesis studies. However, experimental studies have yet to yield consensus as to the exact configuration of the catalytic metal cluster and its ligation scheme. Computational modeling studies, including density functional (DFT) theory combined with quantum mechanics/molecular mechanics (QM/MM) hybrid methods for explicitly including the influence of the surrounding protein, have proposed chemically satisfactory models of the fully ligated OEC within PSII that are maximally consistent with experimental results. The inorganic core of these models is similar to the crystallographic model upon which they were based but comprises important modifications due to structural refinement, hydration and proteinaceous ligation which improve agreement with a wide range of experimental data. The computational models are useful for rationalizing spectroscopic and crystallographic results and for building a complete structure-based mechanism of water-splitting in PSII as described by the intermediate oxidation states of the OEC. This review summarizes these recent advances in QM/MM modeling of PSII within the context of recent experimental studies.

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“…The references given as superscripts in the first column refer to the observation of the components, whereas the references indicated as superscripts in the third column refer to the interpretation of their origin. 1 (Noguchi et al 1992), 2 (Arnold and Azzi 1968;Shuvalov and Litvin 1969), 3 (Sonoike et al 1991), 4 (Ichikawa et al 1975Desai et al 1977), 5 (Vass et al 1989Chapman et al 1991), 6 (Rubin and Venediktov 1969;Desai et al 1975;Inoue 1981), 7 (Demeter et al 1985a), 8 (Inoue et al 1977;Rózsa and Demeter 1982), 9 (Ono and Inoue 1991;Kramer et al 1994), 10 (Rubin and Venediktov 1969;Lurie and Bertsch 1974a), 11 (Rutherford et al 1982). If DCMU is added after the excitation the S 3 Q A − recombination also results in the Q band (Demeter et al 1982)), 12 (Arnold and Azzi 1968;Rubin and Venediktov 1969;Lurie and Bertsch 1974a;Inoue 1981), 13 (Rutherford et al 1982;Inoue 1983;Demeter and Vass 1984), 14 (Rubin and Venediktov 1969;Desai et al 1975), 15 (Demeter et al 1993;, 16 (Miranda and Ducruet 1995) The electron source (X − ) for this band is related to reversed electron flow around PS II, 17 (Sane et al 1977;Rózsa et al 1989;Hideg and Vass 1992), 18 (Vass et al 1989;Hideg and Vass 1993), 19 (Ducruet and Vavilin 1999).…”

Section: Tl Components From Photosynthetic Materials May Be Related Ormentioning

confidence: 93%

The history of photosynthetic thermoluminescence

Vass

Discoveries in Photosynthesis

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A fundamental discovery of photosynthetis research in the 1950s was the detection of thermally stimulated light emission from preilluminated photosynthetic material [Arnold W and Sherwood H (1957) Proc Natl Acad Sci USA 43: 105-114]. This phenomenon, called thermoluminescence (TL), is characteristic of a wide range of materials (minerals, semiconductors, inorganic and organic crystals, and complex biological systems), which share the ability of storing radiant energy in thermally stabilized trap states. The original discovery of TL in dried chloroplasts later proved to be a phenomenon common to all photosynthetic organisms: photosynthetic bacteria, cyanobacteria, algae and higher plants, which can be observed in isolated membrane particles, intact chloroplasts and unicellular organisms, and whole leaves. Following the initial observations considerable effort has been devoted to the identification and characterization of photosynthetic TL components. This work has firmly established the participation of various oxidation states of the water-oxidizing complex, the redox-active tyrosines, and the quinone electron acceptors of Photosystem II (PS II) in the generation of photosynthetic glow curves. Since TL characteristics are very sensitive to subtle changes in the redox properties of the involved electron transport components, the TL method has become a powerful tool in probing a wide range of PS II redox reactions and their modifications by environmental stress effects. Here, the main milestones of research in photosynthetic TL are covered until the present day.Abbreviations: ADRY -agents accelerating the deactivation reactions of water-splitting enzyme Y; AG -afterglow; Chl -chlorophyll; DCMU -3-(3,4-dichlorophenyl)-1,1-dimethylurea; DL and DLE -delayed light emission; PS I -Photosystem I; PS II -Photosystem II; TL -thermoluminescence; Tyr-D and Tyr-Z -redox active tyrosines of the PS II reaction center

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The AT thermoluminescence band from Chlamydomonas reinhardtii and the effects of mutagenesis of histidine residues on the donor side of the Photosystem II D1 polypeptide

Cited by 45 publications

References 20 publications

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Computational insights into the O2-evolving complex of photosystem II

The history of photosynthetic thermoluminescence

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