The bacterial and fungal communities associated with Anthurium ssp. leaves: Insights into plant endemism and microbe association

Andrade, Pedro Avelino Maia de; Souza, Adijailton José de; Lira, Simone Possedente de; Assis, Marco Antônio; Berlinck, Roberto G. S.; Andreote, Fernando Dini

doi:10.1016/j.micres.2020.126667

Cited by 8 publications

(9 citation statements)

References 48 publications

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“…Notably, fungal classes like Tremellomycetes and Dothideomycetes have been identified as fungal core taxa in Anthurium spp. [5]. Interestingly, Phoma medicaginis, the causal agent of spring black stem of Medicago truncatula, was identified as a core member of the tomato.…”

Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

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Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness, and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops, and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops; however, little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum, and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over 2 consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi, and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa, and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner. Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves.Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype, and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

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Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

“…Fig 5. Principal component analysis (PCA) of the taxonomic composition of healthy and dysbiotic samples of S. habrochaites and S. corneliomulleri from Canta.…”

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confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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“…Plants provide a wide variety of niches for growth and proliferation for a great diversity of microorganisms, including bacteria, fungi, protists, nematodes, and viruses, which make up the plant microbiota (1,2). These microorganisms can form complex interactions with plants and have key roles in promoting plant productivity and health (3,4).…”

Section: Introductionmentioning

confidence: 99%

“…The plantbacteria interaction has been the basis of bioproducts that promote increased productivity of agricultural crops, especially inoculants with mechanisms that promote plant-growth, nutrient acquisition and increases of plant tolerance and resistance to abiotic stresses and pests attack (5)(6)(7). Most of these interactions occur in the soil-plant interface using specific niches such as the rhizosphere, endosphere and rhizoplane (1,2,8). The rhizosphere consists of the root zone directly influenced by plant exudates, but the microorganisms inhabiting these regions may also interfere in both plant development and soil fertility (5,9).…”

Section: Introductionmentioning

confidence: 99%

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

et al. 2022

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Burkholderia sp. is a bacterial genus extremely versatile in the environment and has been reported for a great potential to promote plant growth via different mechanisms. Here we evaluate the plant growth-promoting mechanisms in twenty-six Burkholderia strains. Strains were evaluated for their ability to promote plant growth by means of: indole-3-acetic acid (IAA) production under different conditions of pH, salt stress and the presence or absence of L-tryptophan; exopolysaccharides (EPS) production and quorum sensing (ALH). The strains were also characterized in terms of their genetic variability and species identification through Sanger sequencing. Then, the bacteria most responsive in the greatest number of plant-growth promotion mechanisms were selected for a corn seed germination test. All bacteria synthesized IAA in medium with 0.0 or 5.0 mM of L-tryptophan in combination with either 1 or 5% of NaCl, and pH values of either 4.5 or 7.2. The EPS production was confirmed for 61.54% of the bacterial strains. Quorum sensing also occurred in 92.3% of the selected bacteria. The Jaccard similarity coefficient revealed 16 clusters with high genetic variability between bacterial strains. Bacterial strains were assigned to seven species: B. anthina, B. cepacia, B. gladioli, B. ambifaria, B. graminis, B. heleia, and Burkholderia spp. The corn seed bacterization did not affect the germination velocity index (GSI), as well as the first count of germinated seeds (FC). However, inoculations formulated with B. heleia strain G28, B. gladioli strain UAGC723, and B. graminis strain UAGC348 promoted significant increases in root length, seedling height and fresh and dry seedling phytomass, respectively. These results indicate the high biotechnological potential of several strains in the genus Burkholderia sp. as seed inoculants, favoring germination and seedling initial development.

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“…Notably, fungal classes like Tremellomycetes and Dothideomycetes have been identified as fungal core taxa in Anthurium spp. (Andrade et al 2021). Interestingly, Phoma medicaginis, the causal agent of spring black stem of Medicago truncatula was identified as a core member of the tomato.…”

Section: Discussionmentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Runge

Ventura

Kemen

et al. 2021

Preprint

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops, however little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over two consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves. Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

show abstract

The bacterial and fungal communities associated with Anthurium ssp. leaves: Insights into plant endemism and microbe association

Cited by 8 publications

References 48 publications

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

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