The basic repeat unit of a<i>Chironomus</i>Balbiani ring gene

Bäumlein, Helmut; Wobus, Ulrich; Gerbi, Susan A.; Kafatos, Fotis C.

doi:10.1093/nar/10.13.3893

Cited by 25 publications

(17 citation statements)

References 17 publications

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“…Southern analysis using that clone as probe revealed periodic "ladders" in genomic DNA lightly digested with various restriction enzymes, and thus indicated that the internal repeats of the BRc gene are tandemly organized. A similar, tandemly repetitive substructure has been demonstrated in the BRI gene of C. tentans (Degelmann and Hollenberg, 1981), and in its BRb homologue of C. thummi (Baumlein et al, 1982).…”

Section: Introductionsupporting

confidence: 60%

“…Parts of the dominant repeat units of BRI and BRb have been sequenced (Degelmann and Hollenberg, 1981;Baumlein et al, 1982), as has a variant of the BRc repeat (Wobus et al, 1980). We now report the complete sequence of the dominant BRc unit, which we have encountered in four tandem copies by extending our characterization of the previously isolated pCthBRc-19 clone.…”

Section: Introductionmentioning

confidence: 84%

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Characterization of a 249-bp tandemly repetitive, satellite-like repeat in the translated portion of Balbiani ring c of Chironomus thummi.

Bäumlein¹,

Wobus²,

Gerbi³

et al. 1982

The EMBO Journal

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A major part of Balbiani ring (BR)c DNA of Chironomus thummi consists of tandem 249‐bp repeats which appear to be transcribed and translated into a polypeptide of very unusual composition. Whereas these 2549‐bp repeats are evident by Southern blotting, sequence analysis reveals a finer tandemly repetitive substructure: more than half of the 249‐bp repeat length consists of tandem 24‐bp subrepeats, and these in turn may have been generated from even shorter sequences. Comparisons with partial BRb and BR1 sequences reveal that this hierarchically repetitive sequence structure is typical of BR genes. It resembles the structure of some satellite sequences, suggesting that mechanisms leading to satellite DNA evolution may also operate in the evolution of structural genes.

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Section: Introductionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 84%

Characterization of a 249-bp tandemly repetitive, satellite-like repeat in the translated portion of Balbiani ring c of Chironomus thummi.

Bäumlein¹,

Wobus²,

Gerbi³

et al. 1982

The EMBO Journal

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“…However, C. tentans evidently lacks a characteristic special lobe-specific BR and protein (18,49). The silk protein data base for species that have a special lobe (C. pallidivittatus (50) and C. thummi (51,52)) is small but growing 3 (40), but comparable data for special lobe proteins is hard to come by. Salivary glands in these species are smaller (ϳ1 mm in length), and yields of special lobe poly(A) ϩ RNA (2 ng/lobe) and silk proteins (Ͻ100 ng/lobe) are limited.…”

Section: Resultsmentioning

confidence: 99%

A Cell-specific Glycosylated Silk Protein from Chironomus thummi Salivary Glands

Hoffman

Schmidt

Case

1996

Journal of Biological Chemistry

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The seven-transmembrane segment thrombin receptor (TR) represents the prototype of a putative family of proteolytically cleaved receptors that may include the proteinase activated receptor-2. A panel of somatic cell hybrids retaining distinct portions of human chromosome 5 were used to establish that the human TR gene is present as a single-copy locus within the region 5q11.2 -->q13.3, confirming our previous localization using fluorescent in situ hybridization analysis. To further characterize the TR gene, overlapping clones from a human genomic library were isolated. Genomic analysis confirmed that the TR gene is of limited complexity, spanning approximately 27 kilobases and containing two exons separated by a large approximately 22-kilobase intron. The larger second exon contains the majority of the coding sequence and the thrombin cleavage site, remarkably similar to the organization of the proteinase activated receptor-2 gene in which the putative cleavage site is also contained within the large second exon. Primer extension analysis using two 30-mer oligonucleotide primers known to be contained within the first exon identified the predominant transcription initiation site 351 base pairs upstream from the initiator methionine in both human umbilical vein endothelial and human erythroleukemia cells. Sequence analysis of the 5'-flanking region revealed the TR promoter to be TATA-less, although nucleic acid motifspotentially involved in transcriptional gene regulation were evident and include a GATA motif, octamer enhancer sequences, AP-2-like sites, and Sp1 sites. These data provide evidence for remarkable similarity at the gene level between both proteolytically cleaved receptors described to date.

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“…Balbiani rings have been studied intensively in several species of Chironomidae and these studies have resulted both to an understanding of their molecular structure and to the identification of two huge polypeptides which comprise the main secretory products of the salivary glands of the species studied (Hardy & Pelling, 1980;Hertner et al, 1980;Rydlander & Edstr6m, 1980;B~umlein et al, 1982a, b;J~ckle et al, 1982;Siimegi et al, 1982;Wieslander et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Possible correlations of polypeptides and Balbiani rings in the salivary glands of Drosophila auraria Peng.

Manousis

Kastritsis

1987

Genetica

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Salivary glands of various stocks of Drosophila auraria and some of its close relatives were examined with a variety of electrophoretic techniques both from larval and prepupal stages, and after ecdysterone treatments, in an effort to detect possible translation products of the two Balbiani rings (BR1 and BR2) found in the salivary gland chromosomes of these species. Two polypeptides (P2 and P1), with molecular weights of 12,000 and 53,000, respectively, have been detected, the appearance of which coincides with the presence of BR2. The results do not allow the correlation of BR1 action with any specific polypeptide(s).

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The basic repeat unit of aChironomusBalbiani ring gene

Cited by 25 publications

References 17 publications

Characterization of a 249-bp tandemly repetitive, satellite-like repeat in the translated portion of Balbiani ring c of Chironomus thummi.

Characterization of a 249-bp tandemly repetitive, satellite-like repeat in the translated portion of Balbiani ring c of Chironomus thummi.

A Cell-specific Glycosylated Silk Protein from Chironomus thummi Salivary Glands

Possible correlations of polypeptides and Balbiani rings in the salivary glands of Drosophila auraria Peng.

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