The behaviour of T. 1824 (Evans's blue) in circulating blood and a modified method for the estimation of plasma volume

Abstract

In all determinations of the circulating plasma volume by dye methods, it is. necessary to allow sufficient time to elapse for complete mixing of the dye with the plasma before withdrawing a sample for estimation of the concentration. During the mixing period a certain amount of dye disappears from the circulation, and in order to try to arrive at a value for the mixing time and to determine the loss of dye during this period, dye-disappearance curves have been plotted.

Studies of plasma volume, red cell volume and total blood volume in young growing rats

“…By contrast, Wish, Furth & Storey (1950), in simultaneous measurements in mice, found that the plasma volume estimated with Evans blue was consistently higher by a factor 1-2 than that estimated with '3I1-labelled protein; they attributed the discrepancy to a rapid early loss of dye from the circulation, and corrected their Evans blue data by this factor. A similar rapid initial loss of Evans blue was reported by Cruickshank & Whitfield (1945) in the cat. No such fall in the Evans blue concentration of the plasma can however be observed in the mixing curves of Fig.…”

Blood Volume Alterations in Congestive Heart Failure

“…Furthermore, there is direct NTS IN HEART evidence that the relative cell volume of minute vessels is lower than that of the large vessels. ) are not in-Krogh (29) (32). These objections cannot be applied to I13'-labeled serum albumin.…”

Simultaneous Distribution of T-1824 and I131-Labelled Human Serum Albumin in Man 1

“…Two hypotheses have been advanced to explain the discrepant results obtained with T-1824 and other indicators. First, there might be early abstraction of T-1824 by phagocytosis or staining of tissues (15,24,25). Secondly, a disproportionate loss of T-1824 might occur during mixing by penetration into erythrocytepoor (7-14, 25, 28) or even extravascular channels (16,17,(25)(26)(27)(28)(29).…”