The binding of novel two-color fluorescence probe FA to serum albumins of different species

Ercelen, Sebnem; Klymchenko, Andrey S.; Mély, Yves; Demchenko, Alexander P.

doi:10.1016/j.ijbiomac.2005.02.002

Cited by 66 publications

(23 citation statements)

References 58 publications

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“…The technique based on labeling of a single –SH group has been used in the studies of reorganization and interactions of subunits in α‐crystallin structure [21,22]. The new dyes could also be utilized as protein ligands; for instance, they allowed characterizing the specific drug binding site on serum albumin molecules [23]. With regards to sensing applications of these dyes, there are two possible formats, (1) contact sensing (where the reporter dye is directly involved in interaction with the target molecule) [24] and (2) remote sensing (where the reporter dye is located at a distant site and the sensing signal is transmitted by conformational change of the sensor molecule) [8].…”

Section: Protein Labeling and Incorporation Into Nanostructuresmentioning

confidence: 99%

Visualization and sensing of intermolecular interactions with two‐color fluorescent probes

Demchenko

2006

FEBS Letters

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We developed a new generic fluorescence sensing technology based on the change of relative intensities between two well-separated emission bands of the novel functional 3-hydroxychromone (3HC) dyes. A greatly enhanced self-calibrating wavelength-ratiometric response is obtained to all major types of non-covalent interactions that can be used in sensingto polarity, hydrogen bonding ability and to local electrostatic fields. This technology may find a broad range of applications -from homogeneous assays in solutions to microarrays, microfluidic devices, nanosensors and whole cell imaging systems. It allows transforming micelles or phospholipid vesicles into nanosensor devices. In cellular research a high sensitivity to membrane potentials can be obtained and the membrane changes during apoptosis detected.

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Section: Protein Labeling and Incorporation Into Nanostructuresmentioning

confidence: 99%

Visualization and sensing of intermolecular interactions with two‐color fluorescent probes

Demchenko

2006

FEBS Letters

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“…Among the environmentally sensitive fluorophores, flavones have exhibited classical positive solvatochromism features. 33 , 34 Because the highly polar water molecule is a strong H-bond donor, the fluorescence of flavones can be severely quenched by the intermolecular electron or proton transfer between dyes and water (Figure 1 ). 35 The fluorescence of flavones could be efficiently switched on when they are incorporateded by proteins or lipid membranes into the nonpolar microenvironment in cells.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Flavone-Based Fluorogenic Probes for Quick Wash-Free Mitochondrial Imaging in Living Cells

Liu

Shah

Zhang

et al. 2014

ACS Appl. Mater. Interfaces

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Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.

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“…Despite these similarities, some differences exist in the three-dimensional structure of the albumins. Moreover, their physicochemical properties in solution are quite different, which was demonstrated by different experimental techniques such as dielectric spectroscopy (Moser et al 1966), liquid chromatography (Šoltés and Sebille 1997), electrophoresis (Miller and Gemeiner 1998), viscometry (Monkos 2005a, b), calorimetry and fluorescence anisotropy (Dimitrova et al 2000;Ercelen et al 2005), circular dichroism (Khan and Shabnum 2001) or fluorescence spectroscopy and modeling (Gelamo 2002).…”

Section: Introductionmentioning

confidence: 99%

Temperature behaviour of viscous flow with proteins

Monkos¹

2011

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The paper presents the results of viscosity determinations on aqueous solutions of different mammalian serum albumins at a wide range of concentrations and at temperatures ranging from 278 K to 318 K. On the basis of these measurements and a modified Arrhenius equation, the functional dependence of the solution activation energy of viscous flow on temperature was established. The analysis of the results obtained shows that the activation energy decreases with increasing temperature according to a square function for solutions, water molecules, and the albumins studied. The rate at which the activation energy decreases with increasing temperature is different for each albumin and mainly depends on its hydrodynamic radius.

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The binding of novel two-color fluorescence probe FA to serum albumins of different species

Cited by 66 publications

References 58 publications

Visualization and sensing of intermolecular interactions with two‐color fluorescent probes

Visualization and sensing of intermolecular interactions with two‐color fluorescent probes

Biocompatible Flavone-Based Fluorogenic Probes for Quick Wash-Free Mitochondrial Imaging in Living Cells

Temperature behaviour of viscous flow with proteins

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