The binding, transport and fate of aluminium in biological cells

Exley, Christopher; Mold, Matthew

doi:10.1016/j.jtemb.2014.11.002

Cited by 82 publications

(83 citation statements)

References 50 publications

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“…Previous studies have demonstrated that Lactobacilli could maintain a normal mucosal barrier via modulation of TJ proteins [15,40]. Al exposure damage TJ proteins located between pairs of epithelial cells, which may cause intercellular leakage and promote Al movement across the intestinal barrier [2,41,42]. Moreover, Al induces cell damage and apoptosis, which may lead to leakage in the epithelial layer, causing a greater amount of Al to enter the systemic circulation [42,43].…”

Section: Discussionmentioning

confidence: 99%

“…Al exposure damage TJ proteins located between pairs of epithelial cells, which may cause intercellular leakage and promote Al movement across the intestinal barrier [2,41,42]. Moreover, Al induces cell damage and apoptosis, which may lead to leakage in the epithelial layer, causing a greater amount of Al to enter the systemic circulation [42,43]. Therefore, maintaining the integrity of the gut barrier plays a very important role in inhibiting intestinal Al absorption.…”

Section: Discussionmentioning

confidence: 99%

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Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Zhai

Tian

et al. 2016

Nutrients

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Aluminum (Al) is a ubiquitous metal that can seriously harm the health of animals and humans. In our previous study, we demonstrated that Lactobacillus plantarum CCFM639 can decrease Al burden in the tissues of mice by inhibiting intestinal Al absorption. The main aim of the present research was to investigate whether the protection by the strain is also associated with enhancement of the intestinal barrier, alleviation of oxidative stress and modulation of the inflammatory response. In an in vitro cell model, two protection modes (intervention and therapy) were examined and the results indicated that L. plantarum CCFM639 alleviated Al-induced cytotoxicity. In a mouse model, L. plantarum CCFM639 treatment was found to significantly alleviate oxidative stress in the intestinal tract, regulate the function of the intestinal mucosal immune system, restore the integrity of tight junction proteins and maintain intestinal permeability. These results suggest that in addition to Al sequestration, L. plantarum CCFM639 can also inhibit Al absorption by protecting the intestinal barrier, alleviating Al-induced oxidative stress and inflammatory response. Therefore, L. plantarum CCFM639 has the potential to be a dietary supplement ingredient that provides protection against Al-induced gut injury.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Zhai

Tian

et al. 2016

Nutrients

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“…The intactness of the extracted RNA was assessed on 1% agarose gel and the purity was determined using the NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The RNA integrity number (RIN) [35] was assessed using Bioanalyzer 2100 system (Agilent Technologies, Santa, CA, USA) with RNA Pico Bioanalyzer chip (Agilent Technologies) and the quantification of RNA was done using Qubit 1 ). Then, the cDNA libraries were validated using the Bioanalyzer 2100 system with HS DNA chip (Agilent Technologies).…”

Section: Rna Extractionmentioning

confidence: 99%

Global transcriptomic response ofAnoxybacillussp. SK 3-4 to aluminum exposure

Lim

Thevarajoo

Selvaratnam

et al. 2016

J. Basic Microbiol.

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Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybacillus when the cells were exposed to 600 mg L of aluminum. The transcriptome was sequenced using Illumina MiSeq sequencer. Total of 708 genes were differentially expressed (fold change >2.00) with 316 genes were up-regulated while 347 genes were down-regulated, in comparing to control with no aluminum added in the culture. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of genes encoding for cell metabolism such as glycolysis, sulfur metabolism, cysteine and methionine metabolism were up-regulated; while most of the gene associated with tricarboxylic acid cycle (TCA cycle) and valine, leucine and isoleucine metabolism were down-regulated. In addition, a significant number of the genes encoding ABC transporters, metal ions transporters, and some stress response proteins were also differentially expressed following aluminum exposure. The findings provide further insight and help us to understand on the resistance of Anoxybacillus sp. SK 3-4 toward aluminium.

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“…Many of the naturally occurring biochemical reactions in the plasma and serum (P/S) compartment of blood are not at equilibrium when blood exits the body [1]. In addition, exposure of P/S to air results in a dissolved oxygen concentration [O 2 ( aq )] of approximately 0.25 mM [2, 3]—an O 2 ( aq ) concentration that is much higher than that found in the P/S compartment of blood in vivo , where oxygen is carried on hemoglobin inside red blood cells.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo instability of glycated albumin: A role for autoxidative glycation

Jeffs

Ferdosi

Yassine

et al. 2017

Archives of Biochemistry and Biophysics

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Ex vivo protein modifications occur within plasma and serum (P/S) samples due to prolonged exposure to the thawed state—which includes temperatures above −30 °C. Herein, the ex vivo glycation of human serum albumin from healthy and diabetic subjects was monitored in P/S samples stored for hours to months at −80 °C, −20 °C, and room temperature, as well as in samples subjected to multiple freeze-thaw cycles, incubated at different surface area-to-volume ratios or under different atmospheric compositions. A simple dilute-and-shoot method utilizing trap-and-elute LC-ESI-MS was employed to determine the relative abundances of the glycated forms of albumin—including forms of albumin bearing more than one glucose molecule. Significant increases in glycated albumin were found to occur within hours at room temperature, and within days at −20 °C. These increases continued over a period of 1–2 weeks at room temperature and over 200 days at −20 °C, ultimately resulting in a doubling of glycated albumin in both healthy and diabetic patients. It was also shown that samples stored at lower surface area-to-volume ratios or incubated under a nitrogen atmosphere experienced less rapid glucose adduction of albumin—suggesting a role for oxidative glycation in the ex vivo glycation of albumin.

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The binding, transport and fate of aluminium in biological cells

Cited by 82 publications

References 50 publications

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Potential of Lactobacillus plantarum CCFM639 in Protecting against Aluminum Toxicity Mediated by Intestinal Barrier Function and Oxidative Stress

Global transcriptomic response ofAnoxybacillussp. SK 3-4 to aluminum exposure

Ex vivo instability of glycated albumin: A role for autoxidative glycation

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