The Biocompatibility of a Cellulose Exopolysaccharide Implant in the Rabbit Bladder When Compared With Dextranomer Microspheres Plus Hyaluronic Acid

Lima, Salvador Vilar Correia; Rangel, Artur Eduardo de Oliveira; Lira, Mariana Montenegro de Melo; Pinto, Flávia Cristina Morone; Júnior, Olávio Campos; Sampaio, Francisco J.B.; Aguiar, José Lamartine de Andrade

doi:10.1016/j.urology.2015.02.028

Cited by 13 publications

(20 citation statements)

References 22 publications

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“…BC hydrogel subcutaneous implants were found beneath the hypodermis layer of the skin, a slight inflammatory response was present and the formation of new vessels (neovascularization) was present in the majority of the animals. These effects are supported by previous studies (Rangel et al, 2006;Carvalho Junior et al, 2012;Cordeiro-Barbosa et al, 2012;Lima et al, 2015;Pita et al, 2015;Lima et al, 2017;Cavalcante et al, 2018;Gonçalves-Pimentel et al, 2018).…”

Section: Discussionsupporting

confidence: 87%

“…Experimental and clinical studies suggested that sugarcane bacterial cellulose hydrogel (BC) is a biomaterial useful for a variety of surgical procedures, such as correction of hypospadias and other uses in urologic surgery (Martins et al, 2013;Lima et al, 2015;Lucena et al, 2015;Lima et al, 2017), treatment of fecal incontinence after anal trauma (Cavalcante et al, 2018), treatment of eviscerated eyes (Cordeiro-Barbosa et al, 2012), repair of tympanic membrane perforation (Silveira et al, 2016), vascular surgery procedures (Barros-Marques et al, 2012), pressure injury (Oliveira et al, 2019), orthopedic surgery (Albuquerque et al, 2011), and neurosurgery (Lima et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Silva

Pinto

Oliveira

et al. 2020

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A hydrogel of bacterial cellulose of 0.8%, biopolymer produced from sugarcane molasses and synthesized from a bacteria called Zoogloea sp., was analyzed for its composition and tested by two routes of administration, subcutaneous and intraperitoneal, to clarify the local and systemic adverse effects after skin implantation in Wistar rats (24 males, 24 females, 55 days old). Analysis of two samples of BC films indicates carbon (42.94%; 43.43%), hydrogen (6.73%; 6.76%), nitrogen (0.28%, 0.23%) and oxygen (50.05%, 49.58%). No abnormal behavior, clinical signs of chronic toxicity or inflammation were observed. There was no change in liver injury biomarker levels (ALT, AST and ALP), as well as in renal histology, where it was evaluated to signs of tubular cell injury, glomerular or vascular damage, and renal morphometry, in which it was quantified the number of renal corpuscles, the number of cells per glomerulus and the capsular space area. Thus, the absence of signs and symptoms of toxicity suggests that subcutaneous or intraperitoneal injections of these polymers may be used in clinical situations.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Silva

Pinto

Oliveira

et al. 2020

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“…Lima et al (2015) injected BCG into the bladder wall of adult rabbits to evaluate this material as a bulking agent to treat vesicoureteral reflux [25]. In that study, BCG was compared to Dextranomer Microspheres plus hyaluronic acid according to its biocompatibility and evaluated by histological parameters by the means of morphological and quantitative analysis.…”

Section: Discussionmentioning

confidence: 99%

“…The areas of Dextranomer Microsphere were fragmented but still homogeneous and free of cells or blood vessels. At 11 months after implantation the BCG was remodeled and remained in place with intense cellularity and corresponding vascularization while Dextranomer Microspheres gel presented fragmented without vascular neoformation [25].…”

Section: Discussionmentioning

confidence: 99%

Effects of bacterial cellulose gel on the anorectal resting pressures in rats submitted to anal sphincter injury

Cavalcante

Lima

Souza

et al. 2018

Heliyon

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The aim of this study is to evaluate if a gel of bacterial cellulose gel can revert the loss of anal resting pressure after anorectum sphincter injury in rat model, elected as a model to simulate fecal incontinence. Thirty-nine animals were equally divided into three groups: Control (CG), Sphincter injury plus Saline injection (SG) and Sphincter injury plus Bacterial Cellulose Gel injection (BCG). Anal pressure at rest was assessed for all animal in the three groups using anorectum manometry. Saline and Gel groups were subject to anorectum sphincter injury to reduce the anal pressure at rest. Fifteen days later Saline or Gel was injected into the anorectum, according to their groups. Sixty days later first manometry, the anorectum of all animals were removed and processed histologically. The CG group showed maintenance of their mean anorectal resting pressure levels; SG presented a fall in their mean anorectal resting pressure. The BCG presented a significant elevation of the mean anorectal resting pressure levels, surpassing the pressure of CG. The gel of bacterial cellulose remained at the injection site and was neovascularized, colonized by fibroblasts and dense conjunctive tissue. Those data suggest that BC can be used as a future filling agent treatment for fecal incontinence in clinical trial protocols.

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“…Although relative ease of bio-integration and re-modelling has been documented, there are no reports in the literature on use of these biomaterials for repair of bile duct injuries. 14,[16][17][18] Against this background, use of bacterial cellulose membrane for repair of injuries to the extrahepatic biliary ducts represents a promising and potentially cost-effective treatment alternative (note use of these materials for urological procedures). Hence low levels of toxicity for bacterial cellulose coupled with its biocompatibility and proven benefit in experimental models have encouraged its use as a graft material to repair more complex and surgically challenging defects of the bile ducts.…”

Section: Introductionmentioning

confidence: 99%

Use of bacterial cellulose film for repair of bile duct injury in pigs

et al. 2020

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Background/objective The aim was to evaluate the use of bacterial cellulose film and bile duct autograft in repairing critical common bile duct injury in pigs. Methods A prospective experimental analytical study was carried out on 20 Sus Domesticus, Piau suidae swine, divided into a control group ( n = 10) and an experimental group ( n = 10) divided into two subgroups: bacterial cellulose film E1 and bacterial cellulose film E2 to which bacterial cellulose film was randomly allocated. The control group underwent two complete critical common bile duct sections 10 mm apart, while the experimental group with a single critical common bile duct defect underwent a 10 mm section of the longitudinal shaft with edge resection. The defects in the control group were treated with end-to-end conventional anastomosis using polyglycolic 6–0 surgical thread and the experimental group with bacterial cellulose film by continuous suture using the same material. The animals were clinically evaluated throughout the experiment on days D150 (bacterial cellulose film E1), D225 (control group), and D330 (bacterial cellulose film E2) and by intraoperative ultrasound examination related to histopathological and biochemical findings. Results The intraoperative ultrasonography detected the changes resulting from the common bile duct anastomosis in the control group that produced a considerable incidence of ductal narrowing and obstruction to the biliary flow. In the bacterial cellulose film E2 group, there was an increase in inflammation intensity, granulomatous reaction, fibrosis, and vessels density, without producing bile duct dilation in the ultrasonography assessment. Biochemical analysis of liver enzymes yielded results in the normal range confirming preservation of liver function at the different post-surgery time points. Conclusion Bacterial cellulose film, when used as a graft for bile duct repair, proved to be a biocompatible material that produced a complete healing process and biliary flow continuity.

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The Biocompatibility of a Cellulose Exopolysaccharide Implant in the Rabbit Bladder When Compared With Dextranomer Microspheres Plus Hyaluronic Acid

Cited by 13 publications

References 22 publications

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Effects of bacterial cellulose gel on the anorectal resting pressures in rats submitted to anal sphincter injury

Use of bacterial cellulose film for repair of bile duct injury in pigs

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