The Biocontrol Efficacy of Streptomyces pratensis LMM15 on Botrytis cinerea in Tomato

Abstract

LMM15, an actinomycete with broad spectrum antifungal activity, was isolated from a diseased tomato leaf using the baiting technique. A phylogenetic tree analysis based on similarity percentage of 16S rDNA sequences showed that the bacterium was 97.0% affiliated with the species Streptomyces pratensis. This strain was therefore coded as S. pratensis LMM15. The ferment filtrate of LMM15 had ability to inhibit mycelia growth of Botrytis cinerea and reduce lesion expansion of gray mold on detached leaves and fruits. In greenhouse experiments, both the fresh and dry weights of tomato seedlings were significantly increased with the increased concentrations of total chlorophyll. The incidence of tomato gray mold decreased by 46.35%; this was associated with the increase of proline content and malondialdehyde (MDA) and the changes in defense-related enzymes on tomato leaves when the strain was sprayed on the tomato leaves 24 h prior to inoculation with pathogens. This study showed that the strain S. pratensis LMM15 could be a potential agent for controlling tomato gray mold.

Crop Protection against Botrytis cinerea by Rhizhosphere Biological Control Agent Bacillus velezensis XT1

“…Preventive foliar and root applications of B. velezensis strain XT1 reduce the MDA content in B. cinerea -infected tomato and strawberry plants (XT1+Bc). Although similar results have been obtained using different Bacillus strains and crops [ 92 , 94 ], to our knowledge, no previous studies demonstrate the ability of B. velezensis to reduce MDA levels in plants affected by B. cinerea, a mechanism which might explain the in vivo antifungal activity of strain XT1.…”

“…Apart from hydrogen peroxide, malondialdehyde (MDA) is another parameter associated with plant oxidation status. MDA determines the lipid peroxidation produced by ROS, which directly affects the cellular lipid membrane, leading to oxidative degradation and cell lysis [ 92 , 93 ]. Preventive foliar and root applications of B. velezensis strain XT1 reduce the MDA content in B. cinerea -infected tomato and strawberry plants (XT1+Bc).…”

Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

“…Table S1 for physicochemical properties). Seeds were grown in the 32-well seedling tray (54 cm × 29 cm × 5 cm, 1 seed per well) with tomato seedling substrate in a greenhouse at 25 ± 3 °C and relative humidity of 75% with a 12 h-12 h light-dark cycle 17 . Seedlings with similar height (15 ± 3 cm) were transplanted into pots (diameter 20 cm) filled with 3.2 kg of different substrate per pot according to the treatments: (i) natural loam soil obtained from Hebei Academy of Agriculture and Forestry Sciences where corn was once planted (see Suppl.…”

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

“… Streptomyces pratensis LMM15 had the ability to inhibit mycelia growth of B. cinerea and reduce lesion expansion of grey mould on detached leaves and fruits. The incidence of tomato grey mould was decreased by 46.35 % [75]. Pseudomonas aeruginosa CQ-40 had varying degrees of inhibition on B. cinerea and 12 other phytopathogenic fungi.…”