The biomass, community structure, and spatial distribution of the sedimentary microbiota from a high-energy area of the deep sea

Baird, Bruce H.; Nivens, David E.; Parker, J.H.; White, David C.

doi:10.1016/0198-0149(85)90064-0

Cited by 44 publications

(8 citation statements)

References 22 publications

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“…The PLFA profiles of the present study area are notably different from the reported results of PLFA in sediments of other studies. Significant amounts of polyunsaturated fatty acids were reported in deep-sea and estuarine sediments (1,2), marine sediments (32), and intertidal sediments (40). PLFA profiles in sediments of an Antarctic lake system showed that polyunsaturated fatty acids were present at a significant level (26).…”

Section: Discussionmentioning

confidence: 99%

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

Rajendran

Matsuda

Imamura

et al. 1992

Appl Environ Microbiol

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The distribution of phospholipid ester-linked fatty acids (PLFA) in sediments of eutrophic bays (Hiroshima Bay and Aki Nada) was studied to quantify the microbial biomass, community structure, and nutritional status. A total of 63 fatty acids in the range of C10 to C24 were determined. They consist of saturated fatty acids, branched fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids, and variation was revealed in the relative proportions of these fatty acids in sediments. On the basis of the PLFA concentration in sediments, the calculated microbial biomass showed variation (mean + standard deviation = 0.70 x 108 ± 0.53 x 108 cells per g [dry weight] of sediment) in the eutrophic bays. In sediments, a higher amount of biomass was observed in the coastal area of Hiroshima Bay than that observed in the rest of the bay and adjacent Aki Nada. The microbial community structure of the present study area, as characterized by the PLFA profiles, showed very low percentages of polyunsaturated fatty acids and long-chain fatty acids characteristic of microeukaryotes and terrestrial input, respectively, and high percentages of fatty acids characteristic of bacteria. The distribution of PLFA profiles also showed the relative contribution of both aerobic and anaerobic bacteria, especially sulfate-reducing bacteria, in the study area. The relative proportions of PLFA revealed distinctive differences among the stations of the study area, as is evidenced from six clusters obtained for the PLFA profiles. The results of Tukey's honestly significant difference test further confirmed that the sediments in the coastal area of Hiroshima Bay were significantly enriched by a number of fatty acids when compared with other areas investigated where relatively few fatty acids were present in significant quantities. No marked variation in environmental parameters in the surfaceand bottom-water samples was observed, indicating the absence of any water movement in the study area. Furthermore, low redox potential and the levels of sulfide in the sediment revealed the reduced condition of the sediment. The existing environmental conditions and pollution of the study area were attributed to the observed microbial community structure in the sediments.

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Section: Discussionmentioning

confidence: 99%

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

Rajendran

Matsuda

Imamura

et al. 1992

Appl Environ Microbiol

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“…Federle et al (18) found significant spatial variation at the centimeter and 100-m levels within subtropical estuarine sediments. Baird et al (2), using a replicated sampling scheme unprecedented for deep-sea research, found a similar pattern at a deep-sea site subjected to highenergy currents. Several studies have been conducted using molecular approaches to examine spatial variation in benthic bacterial community composition.…”

Section: Discussionmentioning

confidence: 86%

Biome-Level Biogeography of Streambed Microbiota

Findlay

Yeates

Hullar

et al. 2008

Appl Environ Microbiol

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A field study was conducted to determine the microbial community structures of streambed sediments across diverse geographic and climatic areas. Sediment samples were collected from three adjacent headwater forest streams within three biomes, eastern deciduous (Pennsylvania), southeastern coniferous (New Jersey), and tropical evergreen (Guanacaste, Costa Rica), to assess whether there is biome control of stream microbial community structure. Bacterial abundance, microbial biomass, and bacterial and microbial community structures were determined using classical, biochemical, and molecular methods. Microbial biomass, determined using phospholipid phosphate, was significantly greater in the southeastern coniferous biome, likely due to the smaller grain size, higher organic content, and lower levels of physical disturbance of these sediments. Microbial community structure was determined using phospholipid fatty acid (PLFA) profiles and bacterial community structure from terminal restriction fragment length polymorphism and edited (microeukaryotic PLFAs removed) PLFA profiles. Principal component analysis (PCA) was used to investigate patterns in total microbial community structure. The first principal component separated streams based on the importance of phototrophic microeukaryotes within the community, while the second separated southeastern coniferous streams from all others based on increased abundance of fungal PLFAs. PCA also indicated that within-and among-stream variations were small for tropical evergreen streams and large for southeastern coniferous streams. A similar analysis of bacterial community structure indicated that streams within biomes had similar community structures, while each biome possessed a unique streambed community, indicating strong withinbiome control of stream bacterial community structure.

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“…MV-1 and AMB-1 have significantly high monounsaturated fatty acids (C16 plus C18) and high concentration of UQ-n (Fig. 2), reflecting their aerobic respiratory feature (Guckert et al, 1985;Baird et al, 1985). On the contrary, the obligate anaerobes G-20 and GS-15 are characterized by low monounsaturated PLFA and MK-n dominated quinone profiles (Tables 2 and 3 and Fig.…”

Section: Discussionmentioning

confidence: 93%

Microbial respiratory quinones as indicator of ecophysiological redox conditions

2010

Front. Earth Sci. China

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The bacterial respiratory quinones and membrane phospholipid fatty acids (PLFA) were measured to test the biochemical responses to the redox conditions after the respiration of diverse electron acceptors by microorganisms. Shewanella putrefaciens strain CN32 was examined for its growth with O 2 , nitrate, ferrihydrite, ferric citrate, and sulfite as electron acceptors. The same parameters were also measured for Desulfovibrio desulfuricans strain G-20, Geobacter metallireducens strain GS-15, Thioploca spp., two strains of magnetotactic bacteria (Magneteospirilum magnetotactium marine vibrioid strain MV-1 and M. sp. strain AMB-1), and environmental sediments. Microorganisms with aerobic respiratory of oxygen (MV-1 and AMB-1) have high ratios of monounsaturated to saturated straight chain PLFA and ubiquinone to menaquinone ratios; while those that conduct strict anaerobic respirations (G-20 with sulfate and GS-15 with ferric iron) have low ratios of monounsaturated to saturated straight chain PLFA and uniquinone to menaquinone ratios. The facultative respiratory of nitrate (Thioploca) has these parameters in the middle. The ratios of menaquinones to ubiquinones in CN32 cells systematically increase according to the increase of redox potential and bioavalibility of electron acceptors. The correlation between SUQn/SMK-n ratios and redox conditions indicates the structure of respiratory quinone responses sensitively to the microbial ecophysiological conditions.

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The biomass, community structure, and spatial distribution of the sedimentary microbiota from a high-energy area of the deep sea

Cited by 44 publications

References 22 publications

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

Variation in Microbial Biomass and Community Structure in Sediments of Eutrophic Bays as Determined by Phospholipid Ester-Linked Fatty Acids

Biome-Level Biogeography of Streambed Microbiota

Microbial respiratory quinones as indicator of ecophysiological redox conditions

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