The C(-260)>T gene polymorphism in the promoter of the CD14 monocyte receptor gene is not associated with acute myocardial infarction

Abstract

CD surface molecules mediates cell activation and signaling. In particular, CD14 on blood monocytes mediate monocyte/macrophage activation by lipopolysaccharide. Lipopolysaccharide and its receptor, CD14, have been implicated in atherogenesis. It has been recently shown that a C(-260)T polymorphism in the promoter of the CD14 receptor may be a risk factor for coronary artery disease. Recently this association has been questioned because no increased risk was found with the T allele, even in the homozygous state. In the present study we investigated a possible association between the C(-260)T polymorphism in the CD14 promoter and acute myocardial infarction. Two hundred and thrteen patients with and acute myocardial infarction 213 healthy controls were included in the study. Genotype frequencies of the C(-260)T polymorphism in the CD14 promoter were determined by polimerase chain reaction and the amplified product was cleaved with HaeIII. The frequency of the T allele was not significantly different in patients compared with controls. In this study we were not able to detect differences of frequency of the allele T (-260) in the promoter of the CD14 receptor gene in survivors of myocardial infarction and controls.

CD14 C(−260)T gene polymorphism, circulating soluble CD14 levels and arteriosclerosis

“…But this association has not be found in other studies [13,15,16]. Since the deleterious influence of smoking on acute vascular events has been extensively demonstrated [31] and in the light of the interaction observed between (C(À260)!T) polymorphism in CD14 gene and current smoking in sCD14 levels, it would be of interest to determine whether (C(À260)!T) polymorphism in CD14 gene may act as a mediator between the effect of smoking and acute vascular events.…”

“…In human pathology, an association has been found between a polymorphism in the promoter of the receptor CD14 (C(À260)!T) and the risk of acute vascular events (myocardial infarction and stroke) in some selected populations [9][10][11][12]. But this association has not been found in other studies [13][14][15][16] and no significant correlation has been observed with stable coronary heart disease [17]. Also, divergent results have been reported on the relationship between this polymorphism and expression of sCD14 [14,17,18].…”

Pharmacogenomics: A Tool to Prevent and Cure Coronary Heart Disease

“…This support the role of CD14 promoter polymorphism in atheromatous plaque vulnerability. Contrarily, other studies have not corroborated these findings and showed a lack of association between C(-260)T CD14 promoter and susceptibility to atherosclerosis and risk of MI [65][66][67]. Finally, a recent study, performed in a central Italy elderly population with atherosclerotic carotid stenosis, showed an increased TT homozygote frequency of C-260T polymorphism, providing insight into the pathogenetic role of this polymorphism in atherosclerosis [68].…”

Submitral aneurysm

“…Recent studies found no association between allele and genotype distributions of CD14 promoter polymorphism and the risk of MI. [67][68][69][70] The discrepancies between these negative studies and those where an association has been found may be explained by a different ethnic background of the study population and the use of subgroup analysis. Moreover, most of the CD14 studies were cross sectional and not prospective studies.…”