S EVERAL factors have been shown to influence the carotene content of grasses and legumes. Those factors of greatest importance are the stage of growth of the herbage and the seasonal effect characterized by a minimum concentration in summer. The study reported here afforded an opportunity to separate the two factors and show the effect of each.Several workers (10,5,7,4) have reported that the carotene content of pasture grasses is dependent largely upon the growing season and the stage of maturity. Thompson (8) showed that for three strains of alfalfa found in California common, the carotene content decreased at successive stages of growth as the plants approached maturity. In four species of grasses studied by Bird (2), the carotene decreased to approximately one-half that of the short-grass stage by the time the plants began to bloom. In general, reports show that an increase in yield of dry matter in the advanced stages of maturity is obtained at a sacrifice of the concentration of carotene.There is a seasonal decrease in carotene content that is independent of the stage of growth. Atkeson, et al. ( l) reported that, in spring and early summer, plants were relatively high in carotene, with a decrease occurring during the later summer months. The decrease was attributed to drought and high midsummer temperatures in combination with drought. In the four species of grasses studied by Bird (2), when the grasses were cut at the short-grass stage at six intervals throughout the season, the carotene content reached the lowest level in July. After that time, there was an increase in carotene concentration until fall, when values approached those of spring. In a study of weekly variation in carotene content of various herbages during the season, Snyder and Moore (7) found that first cutting alfalfa in Michigan declined from 432 ppm at an immature stage (April 25) to 171 ppm at hay stage on June 20. The second crop declined from 318 ppm at 17 days recovery (July 7) to 132 ppm at hay stage on August 16. The immature third crop decreased from 367 ppm on August 23 to 284 ppm at the three-fourths bloom stage on September 26. It was concluded that the carotene content of the herbages studied was greater at earlier stages of growth, and the carotene content showed a progressive decrease as the plants matured except where affected by factors governing the rate of growth.This paper presents the results of a study designed to determine the cutting treatments or combinations of treatments which would produce the greatest seasonal yield of carotene.
Materials and MethodsThe study was conducted on Ladak alfalfa at the Irrigation Experiment Station, Prosser, Wash. The climatic and soil condi· tions, design, management, and harvesting methods were previously reported with the results of various treatments on yields (3). The plots were seeded in 1946, and in 1947-48 were subjected to spring-clipping treatments of no clipping and clipping at average heights of 4, 7, and 12 inches in combination with intervals between cuttings of 25...