The cellular antioxidant activity in red blood cells (CAA-RBC): A new approach to bioavailability and synergy of phytochemicals and botanical extracts

Blasa, Manuela; Angelino, Donato; Gennari, Lorenzo; Ninfali, Paolino

doi:10.1016/j.foodchem.2010.09.065

Cited by 76 publications

(57 citation statements)

References 26 publications

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“…However, to our knowledge, studies using cell-based assays to assess synergistic action between antioxidants are still not commonly used (MURAKAMI et al, 2003;BLASA et al, 2011). Cell-based assays are considered to provide a more biologically relevant protocol to measure TAA than chemical tests, as they address issues of uptake, distribution, and metabolism.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the antioxidant properties of tomato extracts: A synergistic approach using in vitro chemical tests and cell-based assays

García‐Alonso¹,

Navarro‐González²,

Ros³

et al. 2015

Acta Alimentaria

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The aim of this research was to assess the total antioxidant activity (TAA) of lipophilic (L extr ) and hydrophilic (H extr ) tomato extracts using in vitro chemical tests and cell-based assays, focusing on possible synergistic actions between tomato antioxidants. Both H extr and L extr were HPLC analysed for their carotenoids, phenolic compounds, and ascorbic acid contents. For the evaluation of TAA, extracts were assayed alone or in combination using in vitro chemical tests (TEAC, FRAP) and cell-based (CAA) assays using human hepatoma (HepG2) and human histiocytic lymphoma (U937) cells. The only carotenoid detected in L extr was lycopene, while a mixture of phenolic compounds (chlorogenic acid, caffeic acid, and rutin) was identifi ed in H extr . Ascorbic acid was not found either in H extr or in L extr . Upon extract combination (1:1, v/v), the FRAP assay revealed additive action between L extr and H extr , whilst a slight synergistic action was observed in TAA as measured by the TEAC assay. Synergistic action was better revealed when TAA was analysed using either U937 or HepG2 cells. This could be explained by the presence of a multiphase media (cell membrane and extra-and intracellular media) that might facilitate the distribution and interaction of antioxidants with different polarities and different mechanisms of action.

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Section: Resultsmentioning

confidence: 99%

Assessment of the antioxidant properties of tomato extracts: A synergistic approach using in vitro chemical tests and cell-based assays

García‐Alonso¹,

Navarro‐González²,

Ros³

et al. 2015

Acta Alimentaria

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“…Tamarixetin and kaempferide were isolated from thin-film transistor (TFT) their structures were identified on the basis of spectral data (Galeotti et al, 2008;Blasa et al, 2011) and the purity was >98.5%. Quercetin used as internal standard (IS) and its purity was >99.0%.…”

Section: Chemical Reagents and Animalsmentioning

confidence: 99%

Detection of tamarixetin and kaempferide in different tissues by high-performance liquid chromatography in tamarixetin and kaempferide treated rats

Xu¹,

Feng²,

Yan³

2014

J. Med. Plants Res.

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Tamarixetin and kaempferide were considered as the major active constituents of Tamarix chinensis, and had several known bioactivities. An effective and inexpensive high-performance liquid chromatographic method was established and validated for the determination of tamarixetin and kaempferide in rat tissues following a single oral administration. Tissue distribution showed the highest level of tamarixetin was observed in lung, then in heart; and the highest concentration of kaempferide was determined in heart.

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“…Tamarixetin and kaempferide were isolated from TFX, their structures were identified on the basis of spectral data (Galeotti et al, 2008;Blasa, 2011) and the purity was more than 98.5%. Quercetin used as internal standard (IS) and its purity was more than 99.0%.…”

Section: Chemicals and Drugsmentioning

confidence: 99%

The effective method for investigation meridian tropism theory in rats

Feng²,

Guo³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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This present work describes an effective new method for study traditional Chinese medicine (TCM) on meridian tropism (MT) theory, which plays an essential role in clinical selection of TCM according to syndromes and strengthens the therapeutic effects. The new thread included material basis foundation and its tissue distribution study. Xiheliu, the most popular TCM on heart tropism, was investigated by simple and accurate high performance liquid chromatography (HPLC) method. The analysis of plasma after oral administration the total flavonoid of Xiheliu (TFX) exhibited that tamarixetin and kaempferide had the highest concentration and approximately the highest level within 25 min. The mixture of them could last accelerating the urine excretion more than 7 h after a single dose and could not cause the disorder of ion in rats, which was observed in diuretic activity experiment. In view of the reported biological activities was consistent with the effects of Xiheliu, tamarixetin and kaempferide were likely to be the material basis of it. Tissue distribution study showed that the highest level of analytes was in heart, lung, kidney and liver, and most tissues reached maximum level at 30 min post-dose. Since liver was the most important blood-supply tissue, the result of this experiment was in accordance with the MT record of Xiheliu and confirmed that tamarixetin and kaempferide was the material bases of it on MT. This is the first report for the illumination of material basis and the mechanism of Xiheliu on MT by analysis the record of Xiheliu in Compendium of Materia Medica and experimental study.

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The cellular antioxidant activity in red blood cells (CAA-RBC): A new approach to bioavailability and synergy of phytochemicals and botanical extracts

Cited by 76 publications

References 26 publications

Assessment of the antioxidant properties of tomato extracts: A synergistic approach using in vitro chemical tests and cell-based assays

Assessment of the antioxidant properties of tomato extracts: A synergistic approach using in vitro chemical tests and cell-based assays

Detection of tamarixetin and kaempferide in different tissues by high-performance liquid chromatography in tamarixetin and kaempferide treated rats

The effective method for investigation meridian tropism theory in rats

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