The Cellular Uptake Kinetics and Acute Biological Effects of Various Oxovanadium Species: A Comparative Study

Abstract

It is well known that different oxovanadium species can have significantly different biopotencies, including hypoglycemic actions. The basis for the observed differences in the biopotency of different oxovanadium species: vanadate, vanadyl, 1,10-phenanthroline bisperoxovanadate (phen-bpv), 4-methyl 1,10-phenanthroline bisperoxovanadate (mpv) and 4,7-dimethyl 1,10-phenanthroline bisperoxovanadate (dpv), was examined in this study. The cellular uptake kinetics for these oxovanadium species was measured. Phen-bpv and vandyl had the most rapid cellular uptake. Mpv, dpv and vanadate exhibited a much slower uptake kinetics. Stimulation of protein tyrosine phosphorylation (PTP), both the time dependency and the dose dependency, was used as an index for biopotency. Although phen-bpv and vanadyl had the same cellular uptake kinetics, they differed markedly in their ability to stimulate PTP. Structurally similar oxovanadium species, phen-bpv, mpv and dpv demonstrated different uptake kinetics and effects on stimulating PTP. Bioavailability, both in term of cellular uptake and migration or transport to the active site, has been shown to be an important factor, in addition to intrinsic activity of the oxovanadium species, in determining the overall biopotency. Finally, this study demonstrates that variation of the chelating ligand has a profound effect on the physiochemical properties and biological effects of the oxovanadium species.

Decrease of Hypothalamic Neuropeptide Y Gene Expression by Vanadyl Sulfate in Streptozotocin-Induced Diabetic Rats

“…In VOSO 4 -treated STZ-diabetic rats, plasma glucose concentrations were also markedly lower than that of the vehicle-treated group at day of treatment, which is in agreement with the previous view that daily injection of vanadyl sulfate can lower the plasma glucose in STZ-diabetic rats [19]. Vanadium has been found to mimic some aspects of insulin action in vitro [19,20]. However, the mediation by effect of vanadyl sulfate in reversing hyperphagia seems unlikely because insulin was absent in this animal model.…”