The challenge of herbicide resistance around the world: a current summary

Peterson, Mark A.; Collavo, Alberto; Ovejero, R. F. Lopez; Shivrain, Vinod K.; Walsh, Michael

doi:10.1002/ps.4821

Cited by 265 publications

(225 citation statements)

References 118 publications

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“…South America cultivates > 25% of the total planted area in the world, and the two major row crops, soybean and corn, are annually planted on 59 and 21 million ha, respectively . Most South American farmers (> 90%) adopt a no‐till system and transgenic glyphosate‐resistant (GR) corn and soybean, which can include up to seven glyphosate applications a year . Glyphosate ( N ‐(phosphonomethyl)glycine) inhibits 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) competing with phosphoenolpyruvate for the binding site .…”

Section: Introductionmentioning

confidence: 99%

“…1 Most South American farmers (> 90%) adopt a no-till system and transgenic glyphosate-resistant (GR) corn and soybean, which can include up to seven glyphosate applications a year. 2,3 Glyphosate (N-(phosphonomethyl)glycine) inhibits 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) competing with phosphoenolpyruvate for the binding site. 4 The GR technology has been very successful for many years in soybean fields from Argentina, Bolivia, Brazil, Paraguay and Uruguay, but the lack of diversity in weed control and the overreliance on a single herbicide have led to the evolution of 36 cases of GR in these countries.…”

Section: Introductionmentioning

confidence: 99%

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A novel TIPT double mutation in EPSPS conferring glyphosate resistance in tetraploid Bidens subalternans

Takano

Fernandes

Adegas

et al. 2019

Pest Management Science

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BACKGROUND Bidens subalternans (greater beggarticks) is a tetraploid and troublesome weed infesting annual crops in most tropical regions of the world. A glyphosate‐resistant (GR) B. subalternans biotype was detected in a soybean field from Paraguay. A series of physiological and molecular analyses were conducted to elucidate its resistance mechanisms. RESULTS The GR biotype had a high level of resistance (> 15‐fold LD50), relative to a glyphosate‐susceptible (GS) biotype. Shikimate accumulation was up to ten‐fold greater for GS compared with GR. We found no differences in sensitivity when plants were treated and kept under lower (10/4 °C) or higher temperatures (25/20 °C). GS and GR had the same relative EPSPS gene copy number, and similar glyphosate absorption and translocation rates. Neither biotype metabolized glyphosate. A double amino acid substitution (TIPT – Thr102Ile and Pro106Thr) was found in only one EPSPS allele from one of the two EPSPS homoeologs present in tetraploid GR B. subalternans. CONCLUSION This is the first report of a TIPT double mutation conferring high levels of glyphosate resistance in a weed species. The presence of both wild‐type and TIPT mutant EPSPS on the polyploid genome of GR B. subalternans may offset a potential fitness cost, requiring additional research to confirm the absence of deleterious effects. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel TIPT double mutation in EPSPS conferring glyphosate resistance in tetraploid Bidens subalternans

Takano

Fernandes

Adegas

et al. 2019

Pest Management Science

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“…7 In many parts of the world, overreliance on glyphosate has selected weed populations that are able to withstand lethal doses of the herbicide. 8 Glyphosate resistance mechanisms commonly involve non-target-site mechanisms (reduced glyphosate absorption, reduced glyphosate translocation, glyphosate metabolism and vacuolar sequestration of glyphosate), and/or alterations in the target-site enzyme (amino acid substitutions in the EPSPS and EPSPS duplication). 9 EPSPS is maximally expressed in the plant meristems and, thus, glyphosate needs to reach the growing points to be effective.…”

Section: Introductionmentioning

confidence: 99%

EPSPS duplication and mutation involved in glyphosate resistance in the allotetraploid weed species Poa annua L.

Brunharo

Morran

Martin

et al. 2019

Pest Management Science

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BACKGROUND Poa annua is a widespread winter annual weed species in California. Recently, poor control of this species with glyphosate was reported by growers in an almond orchard in California with a history of repetitive glyphosate use. The objectives of this research were to evaluate the level of glyphosate resistance in a developed S4 P. annua line (R) and identify the mechanisms of resistance involved. RESULTS Whole‐plant dose–response experiments confirmed glyphosate resistance in R, which required 18‐fold more glyphosate to achieve a 50% growth reduction compared with a susceptible line (S), results that were supported by the lower shikimate accumulation observed in R compared with S. No differences in glyphosate absorption, translocation, or metabolism were observed, suggesting that non‐target‐site mechanisms of resistance are not involved in the resistance phenotype. A missense single nucleotide polymorphism was observed in EPSPS coding position 106 in R, resulting in a leucine to proline substitution. This polymorphism was observed exclusively in P. supina EPSPS homeologs. A seven‐fold increase in the number of copies of EPSPS alleles was observed in R compared with S. CONCLUSIONS We report the first case of glyphosate resistance associated with both EPSPS duplication and target‐site mutation at position 106, leading to high levels of glyphosate resistance in the allotetraploid weed species Poa annua L. Data obtained in this research will be useful for the development of diagnostic tools for rapid glyphosate resistance identification, monitoring and containment. © 2018 Society of Chemical Industry

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“…Unfortunately, resistance is often detected too late, after most individuals in a population have acquired a resistant phenotype (McKenna, 2013). Once this stage is reached, there is little to be done except to implement alternative control measures, which are often less effective (Ghosh, Sarkar, Issa, & Haldar, 2019; Peterson, Collavo, Ovejero, Shivrain, & Walsh, 2018), or stop employing control measures until resistant genotypes are eliminated from the population, which can take tens to hundreds of generations (Andersson & Hughes, 2010; Andersson & Levin, 1999; Christie, Sepúlveda, & Dunlop, 2019).…”

Section: Introductionmentioning

confidence: 99%

Incipient resistance to an effective pesticide results from genetic adaptation and the canalization of gene expression

Yin¹,

Martinez²,

Perkins

et al. 2019

Preprint

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1The resistance of bacteria, disease vectors, and pests to chemical controls has vast ecological, 2 economic, and human-health costs. In most cases, resistance is only detected after non-3 susceptible phenotypes have spread throughout the entire population. Detecting resistance in its 4 incipient stages, by comparison, provides time to implement preventative strategies. Incipient 5 resistance (IR) can be detected by coupling standard toxicology assays with large-scale gene 6 expression experiments. We apply this approach to a system where an invasive parasite, sea 7 lamprey (Petromyzon marinus), has been treated with the highly-effective pesticide 3-8 trifluoromethyl-4-nitrophenol (TFM) for 60 years. Toxicological experiments revealed that 9 lamprey from treated populations did not have higher survival to TFM exposure than lamprey 10 from their native range, demonstrating that full-fledged resistance has not yet evolved. In stark 11 contrast, we find hundreds of genes differentially expressed in response to TFM in the 12 population with the longest history of exposure, many of which relate to TFM's primary mode of 13 action, the uncoupling of oxidative phosphorylation. One gene critical to oxidative 14 phosphorylation, ATP5PB, which encodes subunit b of ATP synthase, was nearly fixed for 15 alternative alleles in comparisons between native and treated populations (FST > 9 SD from the 16 mean). A gene encoding an additional subunit of ATP synthase, ATP5F1B, was canalized for 17 high expression in treated populations, but remained plastic in response to treatment in sea 18 lamprey from the native range. These combined genomic and transcriptomic results illustrate that 19 an adaptive, genetic response to TFM is driving incipient resistance in a damaging pest species. 20 S3). Remarkably, when comparing samples treated with 0.3 mg/L TFM to control samples, we 113 found 336 genes that were differentially expressed (275 upregulated, 61 downregulated in 114 comparison to control individuals) in muscle tissue in the Lake Michigan population, dwarfing 115 the number of differentially expressed genes (DEGs) found in both the Lake Champlain (n = 21) 116 and the Connecticut River (n = 68) populations (Fig. 3A-C; Table S4). No DEGs were shared 117 among all three populations and different population responses were observed when lower 118 concentrations (i.e., 0.2 mg/L) of TFM were applied and when different tissues were examined 119 (Figures S2-S5). 120The primary mode of action for TFM is to uncouple oxidative phosphorylation in the 121 mitochondria resulting in severe ATP depletion and eventual death (25, 26, 32). Of the 336 122 DEGs identified in Lake Michigan, several genes were directly related to this mode of action 123 including CRCM1 (LogFC = 2.60), a calcium release-activated channel protein that controls the 124 influx of calcium into cells when depleted, and PLCD4 (LogFC = 2.41), an enzyme responsible 125 for hydrolyzing phosphatidylinositol 4,5-bisphosphate into two secondary messenger molecules, 126 one of which (...

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The challenge of herbicide resistance around the world: a current summary

Cited by 265 publications

References 118 publications

A novel TIPT double mutation in EPSPS conferring glyphosate resistance in tetraploid Bidens subalternans

A novel TIPT double mutation in EPSPS conferring glyphosate resistance in tetraploid Bidens subalternans

EPSPS duplication and mutation involved in glyphosate resistance in the allotetraploid weed species Poa annua L.

Incipient resistance to an effective pesticide results from genetic adaptation and the canalization of gene expression

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