The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment

Nhựt, Dương Tấn; Hương, Nguyễn Thị; Lệ, Bùi Văn; Silva, Jaime Teixeira Da; Fukai, Seiichi; Tanaka, Michio

doi:10.1080/14620316.2002.11511461

Cited by 27 publications

(6 citation statements)

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“…For further improvements, other factors could be taken into account, such as hormonal or light pretreatments (Julliard et al 1992;Nhut et al 2000), the age of the mother plant and the medium pH (Nhut et al 2002), the addition of AgNO 3 (Akasaka- Kennedy et al 2005) or of various sugars, but also more specific factors such as tTCL explant thickness or position along the organ (Nhut et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Ghnaya

Charles

Branchard

2007

Plant Cell Tiss Organ Cult

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The present work describes a procedure that allows for the easy and rapid induction of caulogenesis in four cultivars of Brassica napus L. from transversal Thin Cell Layers (tTCLs). In order to investigate the regeneration ability of this crop, the effects of genotype, explant source and culture medium were examined on shoot regeneration. The tTCL explants were excised from hypocotyl and petiole of 2-week-old seedlings and cultured on a solid basal MS medium supplemented with a-naphthaleneacetic acid (NAA: 0.1-0.4 mg l -1 ), 6-benzylamino-purine (BAP: 1-4 mg l -1 ) and sucrose (20-40 g l -1 ). A significant genotypic effect was observed between the four cvs; Jumbo and Drakkar displayed higher capacities to produce shoots than Pactol and Cossair. Regeneration commenced earlier and the percentage of shoot-producing explants as well as the number of shoots per regenerating explant was greater. The comparison between the regeneration ability of different explants showed that the hypocotyls exhibited a high rate of shoot organogenesis when they were cultured on MS medium supplemented with 3 mg l -1 BAP, 0.3 mg l -1 NAA and 30 g l -1 sucrose. Adventitious shoot buds developed from 46% of the tTCLs, with a mean of 7.5 buds. Furthermore, the method was fast with shoot formation occurring by 7 days culture. Plantlets regenerated from all shoots and developed normally. The regenerated plants were fertile and identical to source plants.

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Section: Discussionmentioning

confidence: 99%

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Ghnaya

Charles

Branchard

2007

Plant Cell Tiss Organ Cult

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“…1). Similarly, in shoot- forming cultures of Lilium multiflorum, increasing PPFD during pre-culture of protocorm-like bodies, raised shoot production (Nhut et al 2002). Dahleen (1999) observed differences in the regeneration capacity of embryoderived callus in barley depending on the planting date, and this was related to the solar radiation being high PPFD necessary to allow sufficient regeneration of somatic embryos.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 91%

In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants

Mingozzi¹,

Morini²

2009

Biologia plant.

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The effect of in vitro cultivation of donor shoots on subsequent morphogenesis in leaf explants of quince (Cydonia oblonga Mill.) clone BA29 was investigated. Proliferating donor shoots were cultured in ventilated or closed vessels under different photosynthetic photon flux densities (PPFD; 200 and 100 µmol m -2 s -1 ) with 0, 15, 30 g dm -3 sucrose. Shoots grown in ventilated vessels, especially with sucrose at 15 or 30 g dm -3 , were better developed with fully expanded leaves compared to those in standard closed vessels. Leaves collected from pre-treated donor shoots were used to assess regeneration capacity. Somatic embryo production was highest in leaves harvested from shoots cultured in closed vessels with 30 g dm -3 sucrose and in ventilated vessels with 15 and 30 g dm -3 sucrose and under high PPFD which was, in comparison with the control treatment (closed vessel, 30 g dm -3 sucrose and low PPFD), about 2 to 2.5 times higher. A similar response was observed for root regeneration.

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“…There are many studies on the selection of suitable nutrient media through the tissue culture of Lilium. These studies include studies on plant growth regulators [29][30][31][32], photoperiod application [33][34][35][36][37], explant size [29,30,33,36,38] and sugar concentration [29,30,33,36,38]. Altan and Bürün [39] reported that the MS medium supplemented with 0.1 mg L -1 NAA+ 0.01 mg L -1 BA, 30 g L -1 sugar and 8 g L -1 agar used was optimal in experiments for micropropagation of L. candidum.…”

Section: Determination Of Genetic Stabilitymentioning

confidence: 99%

Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR

Tokgöz

Altan

2020

International Journal of Secondary Metabolite

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Natural populations of Lilium candidum L. are remarkably affected by biotic and abiotic factors therefore there is a requirement to develop effective micropropagation protocol to provide mass production, multiplication and conservation of these plants. For this reason, this study was aimed to develop an efficient micropropagation method for multiple shoot production via somatic embryogenesis induced from L. candidum stem bulbils and also to determine the genetic stability of in vitro grown plants using SSR markers. The obtained results of this study are the first comprehensive reports including an investigation of genetic fidelity on somatic embryogenesis of L. candidum. After surface sterilization of bulbils, the calculated regeneration percentage of them was 89.5% and the callus induction was achieved using leaf segments of in vitro grown bulbils. The well formed somatic embryos were obtained from smooth whitish-yellow colored calli and these somatic embryos produced well formed healthy L. candidum seedlings with adventitious roots. All rooted seedlings were easily adapted to greenhouse conditions and the genetic stability of in vitro grown seedlings were determined by using SSR-PCR technique and it was calculated as 100%.

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The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment

Abstract: 2002) The changes in shoot regeneration potential of protocorm-like bodies derived from Lilium longiflorum young stem explants exposed to medium volume, pH, light intensity and sucrose concentration pretreatment

Cited by 27 publications

References 0 publications

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L.

In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants

Callus Induction and Micropropagation of Lilium candidum L. Using Stem Bulbils and Confirmation of Genetic Stability via SSR-PCR

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