The characterization of the mitochondrial genome ofCalineuria stigmatica(Plecoptera: Perlidae)

Abstract

The nearly complete sequence of the mitochondrial DNA of Calineuria stigmatica has been completed and annotated in this study. The circular genome is 15,070 bp in length with an A þ T content of 61.8% and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The control region can only be assembled partially. All PCGs use normal start codon ATN, while COI, ND1, and ND5 use CCG, TTG, and GTG as start codon, respectively. Meanwhile, 10 PCGs use the typical termination codons TAN, except COII, ND4, ND5, which stopped with the incomplete terminaton signal T--. Based on 13 PCGs and two rRNAs using the Bayesian (BI) method supported that C. stigmatica was closely grouped with four other Acroneuriinae species. Our results provide basic data for further study of phylogeny in Plecoptera. ARTICLE HISTORY

The complete mitochondrial genome analysis of the stonefly, Flavoperla sp. (Plecoptera: Perlidae)

“…This genus includes 15 species in the world, including six species from China (Chen 2019;DeWalt et al 2019;Liu et al 2019). Only five mitochondrial genomes in the Acroneuriinae were sequenced and published to date (Huang et al 2015;Cao, Wang, Zhang et al 2019;. Here, we sequenced and annotated the mitochondrial genome of Flavoperla sp.…”

Characterization of the complete mitochondrial genome of a stonefly, Perlesta teaysia (Plecoptera: Perlidae)

“…The genus Perlesta Banks, 1906 of the subfamily Acroneuriinae Klap alek, 1914 of Perlidae includes 33 species currently, of which only two are known from China (Wu 1938(Wu , 1948Mur anyi and Li 2016;DeWalt et al 2019). Although a few mitochondrial genomes of the Acroneuriinae have been previously published (Huang et al 2015;Cao, Wang, Zhang, et al 2019;Li et al 2019;Wang et al 2019) and listed in GenBank database, the mitochondrial genome of Perlesta is unavailable. Herein, we sequenced and annotated the complete mitochondrial genome sequence of the Nearctic P. teaysia Kirchner & Kondratieff, 1997 (GenBank accession number MN627432) by Illumina sequencing for the first time.…”