The cholesterol-lowering activity of Averrhoa bilimbi L. leaves ethyl acetate fraction in hypercholesterolemic model

Abstract

Averrhoa bilimbi L. is used widely as spices and traditional medicine, which contained several chemical compounds such as alkaloid, flavonoid, saponin, and tannin. The aim of this study is to discover the best dose of ethyl acetate fraction from A.bilimbi leaves, which have pharmacology activity on total cholesterol and triglycerides in the hypercholesterolemic model. Thirty adult male white mice were divided into six groups; each group was containing five animals. Five groups were given high-fat diet foods and PTU induced the animals to obtain hypercholesterolemia with a frequency of 1 time a day orally for 14 days, while the normal group was given vehicle (Na CMC 5%) and standard feed. On the 15 th day, the normal group was given vehicle (Na CMC 5%), negative control group was given highfat feed, a positive control group was given atorvastatin dose 0.26 mg/kg BW, and another group was given ethyl acetate fraction of A.bilimbi leaves dose 200, 400, and 800 mg/kg BW orally. All animals were treated until the 28 th day. On day 29, the blood were taken to determine the total cholesterol andtriglyceride levels using clinical photometer 5010 v5+. The data were statistically analyzed using a one-way ANOVA followed by the Duncan Multiple Range Test.. This study indicates that administration of ethyl acetate fraction from A.bilimbi leaves can affect the total cholesterol levels and triglycerides in hypercholesterolemic mice (P<0.05). Ethyl acetate fraction dose 200 mg/kg BW shows the best activity in decreasing total cholesterol levels and triglycerides on mice hypercholesterolemic model (P<0.05).

Sub-acute Toxicity Study of Bitter Leaf (Vernonia amygdalina Del.) Aqueous Fraction on Haematological Parameters

“…[18][19][20][21][22][23] The presence of alkaloids and other bioactive compounds invokes the medicinal characteristics of the plants and their therapeutic use in various pharmacopoeias. 24,25 Sub-acute toxicity, animal observation, body weight, food intake and water consumption measurement A sub-acute oral toxicity study in BALB/c mice indicated zero mortality after repeated VALAF administration for 21 days. There was, however, a change in behaviour, such as grooming and sleeping.…”

Roselle Calyx (Hibiscus Sabdariffa. L) as an Anti-Diabetic: Ethyl Acetate Fraction Reduce Fasting Blood Glucose Total Cholesterol and Repair Pancreas Function on Diabetic Model

“…On day 0, 1, 3 and 5 the blood was taken from the ocular sinus and centrifuged at 3000 rpm for 10 min, with the plasma remained. Total cholesterol (TC) and triglyceride (TG), levels in plasma were analyzed according to manufacturer procedures (Sigma-Aldrich, St. Louis, USA) [19].…”