The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage

Zhang, Ling; Zhang, Qian; Jones, Kristi L.; Patel, Mausam; Gong, Feng

doi:10.4161/cc.8.23.10115

Cited by 50 publications

(58 citation statements)

References 30 publications

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“…Inhibition of Ku70, Ku86, TOP2␤, and PARP1 by siRNA led to reduced GRand ER-mediated transcriptional activation in SW-13 and MCF7 cells, respectively. Interestingly, our data further establish the functional link between DNA repair and transcriptional regulation by nuclear receptors and the SWI/SNF chromatin-remodeling complex (18,(67)(68)(69).…”

Section: Figmentioning

confidence: 91%

Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86

Trotter

King

Archer

2015

Molecular and Cellular Biology

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BRG1, the central ATPase of the human SWI/SNF complex, is critical for biological functions, including nuclear receptor (NR)-regulated transcription. Analysis of BRG1 mutants demonstrated that functional motifs outside the ATPase domain are important for transcriptional activity. In the course of experiments examining protein interactions mediated through these domains, Ku70 (XRCC6) was found to associate with a BRG1 fragment encompassing the conserved helicase-SANT-associated (HSA) and BRK domains of BRG1. Subsequent transcriptional activation assays and chromatin immunoprecipitation studies showed that Ku70/86 and components of the topoisomerase II␤ (TOP2␤)/poly(ADP ribose) polymerase 1 (PARP1) complex are necessary for NR-mediated SWI/SNF-dependent transcriptional activation from endogenous promoters. In addition to establishing Ku-BRG1 binding and TOP2␤/PARP1 recruitment by nuclear receptor transactivation, we demonstrate that the transient appearance of glucocorticoid receptor (GR)/BRG1-dependent, TOP2␤-mediated double-strand DNA breaks is required for efficient GR-stimulated transcription. Taken together, these results suggest that a direct interaction between Ku70/86 and BRG1 brings together SWI/SNF remodeling capabilities and TOP2␤ activity to enhance the transcriptional response to hormone stimulation.

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Section: Figmentioning

confidence: 91%

Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86

Trotter

King

Archer

2015

Molecular and Cellular Biology

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“…*P≤0.05 ( paired Student's t-test), cDNA control vector versus Pax6 and/or Hsf4 cDNA. chromatin remodeling participates in DNA repair (Erdel and Rippe, 2011;Lans et al, 2012;Zhang et al, 2009). SWI/SNF complexes are known to be recruited to phosphorylated H2AX via the interaction between the Brg1 bromodomain and acetylated lysines in histone tails (Lee et al, 2010).…”

Section: Kip2mentioning

confidence: 99%

Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation

Limi

McGreal

et al. 2016

Development

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Ocular lens morphogenesis is a model for investigating mechanisms of cellular differentiation, spatial and temporal gene expression control, and chromatin regulation. Brg1 (Smarca4) and Snf2h (Smarca5) are catalytic subunits of distinct ATP-dependent chromatin remodeling complexes implicated in transcriptional regulation. Previous studies have shown that Brg1 regulates both lens fiber cell differentiation and organized degradation of their nuclei (denucleation). Here, we employed a conditional Snf2h flox mouse model to probe the cellular and molecular mechanisms of lens formation. Depletion of Snf2h induces premature and expanded differentiation of lens precursor cells forming the lens vesicle, implicating Snf2h as a key regulator of lens vesicle polarity through spatial control of Prox1, Jag1, p27Kip1 (Cdkn1b) and p57Kip2 (Cdkn1c) gene expression. The abnormal Snf2h −/− fiber cells also retain their nuclei. RNA profiling of Snf2h −/− and Brg1 −/− eyes revealed differences in multiple transcripts, including prominent downregulation of those encoding Hsf4 and DNase IIβ, which are implicated in the denucleation process. In summary, our data suggest that Snf2h is essential for the establishment of lens vesicle polarity, partitioning of prospective lens epithelial and fiber cell compartments, lens fiber cell differentiation, and lens fiber cell nuclear degradation.

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“…Intriguingly, BRG1/BRM, but none of the other subunits, is also important to the UVC response in germ cells, suggesting that the involvement of individual SWI/SNF subunits may differ between cell types. Interestingly, UVC hypersensitivity resulting from BRG1 inactivation depends on the presence of the checkpoint protein TP53, extending the complexity of the involvement of BRG1 in UVC-induced DNA damage response [83]. Several lines of evidence suggest that recruitment of factors like SWI/SNF and their functional participation help to recruit downstream factors for processing DNA damage.…”

Section: Swi/snfmentioning

confidence: 99%

“…In mammals, the SWI/SNF ATPase subunit BRG1/SMARCA4 stimulates efficient repair of CPDs but not of 6-4PPs. For Example, BRG1 interacts with XPC and it is recruited to an UVC lesion in a DDB2 [83] and XPC [76] dependent manner. BRG1, in turn, modulates UVC-induced chromatin remodeling and XPC stability and subsequently promotes damage excision and repair synthesis by facilitating the recruitment of XPG and PCNA to the damage site [76], suggesting the essential role of Brg1 in prompt elimination of UVC-induced DNA damage by NER in mammalian cells.…”

Section: Swi/snfmentioning

confidence: 99%

Chromatin Remodeling in Nucleotide Excision Repair in Mammalian Cells

Martínez‐López¹,

Méndez-Acuña²,

Bervejillo³

et al. 2013

New Research Directions in DNA Repair

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The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage

Abstract: Gong (2009) The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage, Cell Cycle, 8:23, 3953-3959,

Cited by 50 publications

References 30 publications

Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86

Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86

Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation

Chromatin Remodeling in Nucleotide Excision Repair in Mammalian Cells

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