The chromosomal location of genes for elongation factor Tu and ribosomal protein S10 in the cyanobacterium Spirulina platensis provides clues to the ancestral organization of the str and S10 operons in prokaryotes

Sanangelantoni, Anna Maria; Tiboni, Orsola

doi:10.1099/00221287-139-11-2579

Cited by 13 publications

(6 citation statements)

References 33 publications

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“…Unfortunately, the only information available for the cyanobacteria (the putative prokaryotic ancestor of plastids) concerns the str operon [24]. Land plant and green algal plastids also contain a large cluster of 10 ribosomal protein genes (together with infA and rpoA) corresponding to the SlO/spc/alpha operons of E. coli [for review see 25,26].…”

Section: Gene Arrangementmentioning

confidence: 99%

The large ribosomal protein gene cluster of a cryptomonad plastid: Gene organization, sequence and evolutionary implications

1997

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The complete sequence of the major ribosomal protein gene cluster of the plastid genome of the cryptomonad alga Guillardia theta (formerly Cryptomonas Φ) is presented. The ribosomal protein genes (corresponding to the SIO, spc, alpha and L13/S9 operons of E. coli are found upstream of the previously reported plastid str operon, and transcribed in the same orientation. The genes are very tightly packed with as little as two nucleotides between the rpll4 and rpl24 genes. The gene arrangement is very similar to that reported for the rhodophyte alga, Porphyra purpurea, and the chromophyte diatom, Odontella sinensis, indicating a close evolutionary relationship between these groups of algae. Northern analysis indicates that the 29 genes1 are arranged as one operon and are transcribed as a single mRNA that is subsequently processed into smaller transcripts.

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Section: Gene Arrangementmentioning

confidence: 99%

The large ribosomal protein gene cluster of a cryptomonad plastid: Gene organization, sequence and evolutionary implications

1997

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“…[5], Pisum sativum (P. sativum) [9], and Solanum tuberosum (S. tubersosum) [10], the cyanellar sequence of Cyanophora paradoxa (C. paradoxa) [24], the cyanobacterial sequence of Spirulina platensis (S. platensis) [25], and the eubacterial sequence of Escherichia coil (E. coli) [26] are shown. Homologous amino acids between the different RPSIO polypeptides are highlighted by inverse contrast.…”

mentioning

confidence: 99%

Transfer of rps10 from the mitochondrion to the nucleus in Arabidopsis thaliana: evidence for RNA‐mediated transfer and exon shuffling at the integration site

Wischmann

Schuster

1995

FEBS Letters

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“…The same four genes are also arranged in the same order in archaea (7,25). Furthermore, the gene encoding ribosomal protein S10 (rpsJ) is closely linked to the tuf gene in many species (22,38,48,64). Taken together, these data suggest that the location of the tuf gene within the str motif represents an ancient ancestral organization.…”

Section: Discussionmentioning

confidence: 48%

A chimeric disposition of the elongation factor genes in Rickettsia prowazekii

et al. 1996

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An exceptional disposition of the elongation factor genes is observed in Rickettsia prowazekii, in which there is only one tuf gene, which is distant from the lone fus gene. In contrast, the closely related bacterium Agrobacterium tumefaciens has the normal bacterial arrangement of two tuf genes, of which one is tightly linked to the fus gene. Analysis of the flanking sequences of the single tuf gene in R. prowazekii shows that it is preceded by two of the four tRNA genes located in the 5 region of the Escherichia coli tufB gene and that it is followed by rpsJ as well as associated ribosomal protein genes, which in E. coli are located downstream of the tufA gene. The fus gene is located within the str operon and is followed by one tRNA gene as well as by the genes secE and nusG, which are located in the 3 region of tufB in E. coli. This atypical disposition of genes suggests that intrachromosomal recombination between duplicated tuf genes has contributed to the evolution of the unique genomic architecture of R. prowazekii.Bacteria that are obligate parasites of eucaryotic cells, such as Mycoplasma, Coxiella, Chlamydia, and Rickettsia spp., are often relatively small, with genomes of the size of 1 Mb or less. The phylogenetic placement of these bacteria on the basis of their rRNA genes indicate that they are independent descendents of diverse bacteria with much larger genomes (40). In order to examine the hypothesis that reductive evolution of the genomes of obligate parasitic bacteria proceeds through intrachromosomal recombination at duplicated genes, we have compared the dispositions of the genes coding for the elongation factors in the alpha proteobacteria Rickettsia prowazekii and Agrobacterium tumefaciens.These closely related bacteria differ drastically in their lifestyles and genomic architectures. R. prowazekii is an obligate intracellular parasite causing typhus in humans, and it has a single, circular genome of 1.1 Mb (16). In contrast, A. tumefaciens is a free-living soil bacterium that is also responsible for the development of crown gall and hairy root diseases of dicotyledonous plants (13,63). It has a large, complex genome containing four replicons: two chromosomes of 3.0 and 2.1 Mb, a 450-kb cryptic plasmid, and the 200-kb Ti plasmid (1).The gene for elongation factor Tu (EF-Tu) is present in two copies in the genome of Escherichia coli (18, 23) as well as in many other proteobacterial genomes (54). Furthermore, the two tuf genes are normally associated with characteristic flanking sequences. For example, in E. coli, tufA is a member of the str (streptomycin) operon, in which the genes are arranged in the order rpsL, rpsG, fusA, and tufA, whereas tufB is part of the tufB operon, in which four tRNA genes, thrU, tyrU, glyT, and thrT, are positioned at the 5Ј side of the tufB gene (3,46,62). Similar arrangements of the flanking sequences of tuf genes are common in eubacteria and in archaebacteria (6,25,42). Such widely preserved patterns of genomic disposition presumably reflect ancient ancestral pa...

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The chromosomal location of genes for elongation factor Tu and ribosomal protein S10 in the cyanobacterium Spirulina platensis provides clues to the ancestral organization of the str and S10 operons in prokaryotes

Cited by 13 publications

References 33 publications

The large ribosomal protein gene cluster of a cryptomonad plastid: Gene organization, sequence and evolutionary implications

The large ribosomal protein gene cluster of a cryptomonad plastid: Gene organization, sequence and evolutionary implications

Transfer of rps10 from the mitochondrion to the nucleus in Arabidopsis thaliana: evidence for RNA‐mediated transfer and exon shuffling at the integration site

A chimeric disposition of the elongation factor genes in Rickettsia prowazekii

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